Microsatellite analysis of genetic diversity in 3 wild populations of Exopalaemon carinicauda

The fast development of aquaculture over the past decades has made it the main source of fish protein and led to its integration into the global food system. Mostly originating from inland production systems, aquaculture has emerged as strategy to decrease malnutrition in low-income countries. The Nile tilapia (Oreochromis niloticus) was introduced to Madagascar in the 1950s, and is now produced nationally at various scales. Aquaculture mostly relies on fry harvested from wild populations and grow-out in ponds for decades. It has recently been diversified by the introduction of several fast-growing strains. Little is known how local genetic diversity compares to recently introduced strains, although high and comparable levels of genetic diversity have previously been observed for both wild populations and local stocks. Our study compares DNA barcode genetic diversity among eight farms and several strains belonging to three species sampled. DNA-based lineage delimitation methods were applied and resulted in the detection of six well differentiated and highly divergent lineages. A comparison of DNA barcode records to sequences on the Barcode of Life Data System (BOLD) helped to trace the origin of several of them. Both haplotype and nucleotide diversity indices highlight high levels of mitochondrial genetic diversity, with several local strains displaying higher diversity than recently introduced strains. This allows for multiple options to maintain high levels of genetic diversity in broodstock and provides more options for selective breeding programs.

Download Full-text

High genetic diversity and substantial population differentiation in grass carp (Ctenopharyngodon idella) revealed by microsatellite analysis

Aquaculture ◽

10.1016/j.aquaculture.2009.09.008 ◽

2009 ◽

Vol 297 (1-4) ◽

pp. 51-56 ◽

Cited By ~ 44

Author(s):

Feng Liu ◽

Jun-Hong Xia ◽

Zhi-Yi Bai ◽

Jian-Jun Fu ◽

Jia-Le Li ◽

...

Keyword(s):

Genetic Diversity ◽

Grass Carp ◽

Population Differentiation ◽

Microsatellite Analysis ◽

Ctenopharyngodon Idella ◽

High Genetic Diversity ◽

Grass Carp Ctenopharyngodon Idella

Download Full-text

Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha44210391 ◽

2016 ◽

Vol 44 (2) ◽

pp. 431-436 ◽

Cited By ~ 6

Author(s):

Masoumeh YOUSEFIAZARKHANIAN ◽

Ali ASGHARI ◽

Jafar AHMADI ◽

Behvar ASGHARI ◽

Ali Ashraf JAFARI

Keyword(s):

Genetic Diversity ◽

Genetic Polymorphisms ◽

Genomic Dna ◽

Rapd Markers ◽

Genetic Relationships ◽

Genetic Variations ◽

Molecular Techniques ◽

Wild Populations ◽

Similarity Coefficients ◽

The World

The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Neiâ€™s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.

Download Full-text

Genetic variation in native and farmed populations of Tambaqui (Colossoma macropomum) in the Brazilian Amazon: regional discrepancies in farming systems

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-376520130007 ◽

2013 ◽

Vol 85 (4) ◽

pp. 1439-1447 ◽

Cited By ~ 8

Author(s):

Jonas Aguiar ◽

Horacio Schneider ◽

Fatima Gomes ◽

Jeferson Carneiro ◽

Simoni Santos ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Variation ◽

Dna Sequences ◽

Amazon Basin ◽

Farming Systems ◽

Wild Populations ◽

The West ◽

Colossoma Macropomum ◽

Breeding Stock ◽

History Of

The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the states of Amapá, Pará, Piauí, and Rondônia, which recorded only two haplotypes, indicating a long history of endogamy in the breeding stocks used to produce fry. The results of the two studies show two distinct scenarios of tambaqui farming in the Amazon basin, which must be better evaluated in order to guarantee the successful expansion of this activity in the region, and the rest of Brazil, given that the tambaqui and its hybrids are now farmed throughout the country.

Download Full-text