Molecular characterization of complement 9 in Epinephelus coioides and differential expression analysis of classical complement genes following Vibrio alginolyticus challenge

Ecotoxicology ◽  
2020 ◽  
Vol 29 (7) ◽  
pp. 837-845
Author(s):  
Sheng-Wei Luo ◽  
Wei Wei
2018 ◽  
Vol 116 ◽  
pp. 180-188 ◽  
Author(s):  
Yi-Ming Shui ◽  
Shi-Ying Lu ◽  
Xing Guo ◽  
Xi-Lin Liu ◽  
Bao-Quan Fu ◽  
...  

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Matthew Chung ◽  
Vincent M. Bruno ◽  
David A. Rasko ◽  
Christina A. Cuomo ◽  
José F. Muñoz ◽  
...  

AbstractAdvances in transcriptome sequencing allow for simultaneous interrogation of differentially expressed genes from multiple species originating from a single RNA sample, termed dual or multi-species transcriptomics. Compared to single-species differential expression analysis, the design of multi-species differential expression experiments must account for the relative abundances of each organism of interest within the sample, often requiring enrichment methods and yielding differences in total read counts across samples. The analysis of multi-species transcriptomics datasets requires modifications to the alignment, quantification, and downstream analysis steps compared to the single-species analysis pipelines. We describe best practices for multi-species transcriptomics and differential gene expression.


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