Reaction of transgenic Citrus sinensis plants to Citrus tristeza virus infection by Toxoptera citricida

2014 ◽  
Vol 139 (1) ◽  
pp. 151-159 ◽  
Author(s):  
Fabiana R. Muniz ◽  
Amancio Souza ◽  
Ricardo Harakava ◽  
Francisco de Assis Alves Mourão Filho ◽  
Dagmar R. Stach-Machado ◽  
...  
Keyword(s):  
Virus Infection ◽  
Citrus Sinensis ◽  
Citrus Tristeza Virus ◽  
Toxoptera Citricida ◽  
Tristeza Virus ◽  
Transgenic Citrus ◽  
Citrus Tristeza

A possible role of CTV.20 gene methylation in response to Citrus tristeza virus infection

2017 ◽  
Vol 150 (2) ◽  
pp. 527-532 ◽  
Author(s):  
Valentina Fanelli ◽  
C. De Giovanni ◽  
M. Saponari ◽  
P. Leonetti ◽  
L. Ricciardi ◽  
...  
Keyword(s):  
Virus Infection ◽  
Citrus Tristeza Virus ◽  
Gene Methylation ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Breakdown of Cross-Protection of Grapefruit from Decline-Inducing Isolates of Citrus tristeza virus Following Introduction of the Brown Citrus Aphid

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1116-1118 ◽  
Author(s):  
C. A. Powell ◽  
R. R. Pelosi ◽  
P. A. Rundell ◽  
M. Cohen
Keyword(s):  
Immunosorbent Assay ◽  
Citrus Tristeza Virus ◽  
Cross Protection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Experimental Area ◽  
Toxoptera Citricida ◽  
The Cross ◽  
Tristeza Virus ◽  
Citrus Tristeza

A 21-year-old replicated field planting of 84 ‘Ruby Red’ grapefruit trees cross-protected with three mild isolates of Citrus tristeza virus (CTV) was assessed for decline-inducing and non-decline-inducing isolates of the virus 5 years after the brown citrus aphid (BrCA) (Toxoptera citricida Kirkaldy) first was established in the experimental area. Prior to the introduction of the BrCA, the cross-protecting mild isolates had significantly reduced detectable infection with decline-inducing isolates of CTV for 16 years (average infection of 13% in cross-protected trees compared with 67% in unprotected trees). After the introduction of the BrCA, infections with decline-inducing CTV (measured by enzyme-linked immunosorbent assay) were 57, 81, and 71% for trees protected with three mild isolates, respectively, compared with 95% in unprotected trees. These results suggest that the introduction of BrCA accelerated the breakdown of cross-protection against decline-inducing isolates of CTV in grapefruit.

scholarly journals Prevalence of Severe Strains of Citrus Tristeza Virus in Florida Citrus Nurseries

HortScience ◽  
1993 ◽  
Vol 28 (7) ◽  
pp. 699-700 ◽  
Author(s):  
Charles A. Powell ◽  
Robert R. Pelosi
Keyword(s):  
Citrus Sinensis ◽  
Citrus Tristeza Virus ◽  
Severe Strain ◽  
Florida Citrus ◽  
Tristeza Virus ◽  
Citrus Tristeza

Sixty-eight percent of the `Pineapple', 52% of the `Navel', 46% of the `Valencia', 38% of the `Hamlin', and 0% of the `Ambersweet' orange [Citrus sinensis (L.) Osh.] trees in five Florida citrus nurseries were infected with severe strains of citrus tristeza virus (CTV), as demonstrated by reaction with a monoclinal antibody specific for severe strains of the virus. Severe strains of CTV infected 4%, 46%, 76%, 30%, and 48% of the trees at each of the five nurseries, respectively, indicating a considerable difference in severe strain prevalence among the nurseries. Thirty-five percent of the trees in the scion blocks (budwood source) of the nurseries also contained severe strains of CTV.

scholarly journals Seleção de Isolados do Citrus tristeza virus (CTV) Protetivos para Laranja 'Pêra' [Citrus sinensis (l.) Osbeck] no Paraná

2015 ◽  
Author(s):  
Karina Silva dos Santos ◽  
Ana Paula Gonçalves ◽  
Carlos Henrique da Silva Gonçalves ◽  
Tanara Garcia de Novaes ◽  
Rubia de Oliveira Molina
Keyword(s):  
Citrus Sinensis ◽  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Identificação de isolados de Citrus tristeza virus (CTV) protetivos para Citrus sinensis (L.) Osbeck

2018 ◽  
Vol 44 (1) ◽  
pp. 17-22
Author(s):  
Ana Paula Gonçalves ◽  
Karina Silva dos Santos ◽  
Camila de Cassia Silva ◽  
Tanara Garcia de Novaes ◽  
Rúbia de Oliveira Molina
Keyword(s):  
Citrus Sinensis ◽  
Citrus Tristeza Virus ◽  
Rt Pcr ◽  
Tristeza Virus ◽  
Citrus Tristeza

RESUMO O Citrus tristeza virus (CTV) causa significativas perdas na produtividade de laranja doce [Citrus sinensis (L.) Osbeck] e seu controle tem sido realizado principalmente com a premunização. O trabalho teve como objetivo analisar a variabilidade de isolados fortes e fracos de CTV provenientes de plantas de citros inoculadas e mantidas em casa de vegetação e amostras de campo, coletadas em pomar comercial situado no município de Rolândia, PR. Para a determinação da variabilidade e diversidade genética dos isolados foi realizada avaliação dos sintomas e empregadas as técnicas de RT– PCR e RFLP, utilizando os oligonucleotídeos específicos HCP1/HCP2 e posterior sequenciamento dos fragmentos amplificados. Na avaliação de canelura, os isolados mantidos em casa de vegetação induziram sintomas leves, com exceção do isolado severo Capão Bonito. Os sintomas mais severos ocorreram em amostras situadas no campo. De acordo com as análises multivariadas os isolados de CTV tendem a se agrupar conforme a severidade dos sintomas e condições ambientais as quais foram expostas formando agrupamentos distintos entre amostras provenientes do campo e casa de vegetação. O dendrograma gerado a partir do sequenciamento dos isolados e as análises multivariadas revelaram que o isolado proveniente da amostra “Forte Arapongas” apresentou maior similaridade com o controle padrão forte proveniente de Capão Bonito. Os isolados identificados como fracos e provenientes das amostras Pêra IAC e Rolândia 5 apresentaram maior similaridade. Pode-se aferir que plantas hospedeiras mantidas em campo possuem maior variabilidade de isolados.

scholarly journals Increase and Patterns of Spread of Citrus Tristeza Virus Infections in Costa Rica and the Dominican Republic in the Presence of the Brown Citrus Aphid, Toxoptera citricida

1998 ◽  
Vol 88 (7) ◽  
pp. 621-636 ◽  
Author(s):  
T. R. Gottwald ◽  
S. M. Garnsey ◽  
J. Borbón
Keyword(s):  
Costa Rica ◽  
Dominican Republic ◽  
Spatial Autocorrelation ◽  
Citrus Tristeza Virus ◽  
Local Area ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toxoptera Citricida ◽  
Area Of Influence ◽  
Tristeza Virus ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) was monitored for 4 years by monoclonal antibody probes via enzyme-linked immunosorbent assay in four citrus orchards in northern Costa Rica and four orchards in the Dominican Republic following the introduction of the brown citrus aphid, Toxoptera citricida. The Gompertz nonlinear model was selected as the most appropriate in most cases to describe temporal increase of CTV. Ordinary runs analysis for association of CTV-positive trees failed to show a spatial relationship of virus status among immediately adjacent trees within or across rows. The beta-binomial index of dispersion for various quadrat sizes suggested aggregations of CTV-positive trees for all plots within the quadrat sizes tested. Spatial autocorrelation analysis of proximity patterns suggested that aggregation often existed among quadrats of various sizes up to four lag distances; however, significant lag positions discontinuous from the main proximity pattern were rare. Some asymmetry was also detected for some spatial autocorrelation proximity patterns. These results were interpreted to mean that, although CTV-positive trees did not often influence immediately adjacent trees, virus transmission was common within a local area of influence that extended two to eight trees in all directions. Where asymmetry was indicated, this area of influence was somewhat elliptical. The spatial and temporal analyses gave some insight into possible underlying processes of CTV spread in the presence of T. citricida and suggested CTV spread was predominantly to trees within a local area. Patterns of longer-distance spread were not detected within the confines of the plot sizes tested. Longer-distance spread probably exists, but may well be of a complexity beyond the detection ability of the spatial analysis methods employed, or perhaps is on a scale larger than the dimensions of the plots studied.

Citrus tristeza virus (CTV) resistance in transgenic citrus based on virus challenge of protoplasts

2003 ◽  
Vol 39 (6) ◽  
pp. 567-572 ◽  
Author(s):  
O. Olivares-Fuster ◽  
G. H. Fleming ◽  
M. R. Albiach-Marti ◽  
S. Gowda ◽  
W. O. Dawson ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Challenge ◽  
Tristeza Virus ◽  
Transgenic Citrus ◽  
Ctv Resistance ◽  
Citrus Tristeza
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