Biological control of green mould on Agaricus bisporus by a native Bacillus subtilis strain from mushroom compost

2016 ◽  
Vol 148 (3) ◽  
pp. 509-519 ◽  
Author(s):  
Svetlana Milijašević-Marčić ◽  
Miloš Stepanović ◽  
Biljana Todorović ◽  
Bojan Duduk ◽  
Jelena Stepanović ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Agaricus Bisporus ◽  
Subtilis Strain ◽  
Mushroom Compost ◽  
Green Mould

scholarly journals A large-scale study on the effectiveness of a Bacillus subtilis Ch-13-based biofungicide against green mould disease and mushroom yield improvement

2021 ◽  
Vol 36 (2) ◽  
pp. 83-90
Author(s):  
Ivana Potocnik ◽  
Biljana Todorovic ◽  
Svetlana Milijasevic-Marcic ◽  
Jelena Lukovic ◽  
Gabriella Kanizai-Saric ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Untreated Control ◽  
Agaricus Bisporus ◽  
Large Scale ◽  
Natural Infection ◽  
Subtilis Strain ◽  
Cultivated Mushroom ◽  
Green Mould ◽  
Mushroom Yield ◽  
Final Amount

The aim of this study was to test a biofungicide based on Bacillus subtilis Ch-13 and its effectiveness in the control of green mould disease of cultivated mushroom in comparison with the fungicide prochloraz. Biofungicide effectiveness in disease control and impact on yield were evaluated on Agaricus bisporus after its natural infection with Trichoderma aggressivum in a commercial mushroom growing facility. An assay for testing the microbial efficacy of the biofungicide was conducted in two different procedures involving either three or two split doses. The highest statistically significant effectiveness in green mould control was shown by the fungicide prochloraz (71.43%), followed by the biofungicide applied in tree split doses (53.57%), and finally its two doses (45.46%). The biofungicide significantly improved yield in comparison with an untreated control and the fungicide prochloraz. Three split applications of B. subtilis strain Ch-13 enhanced mushroom yield to a larger extent than its two split doses, although the same final amount was used in both procedures. Biofungicide application in three split doses increased the total mass of harvested mushrooms 8.41% compared to the untreated control, and 10.53% compared to the fungicide prochloraz. These results implied that the biofungicide should be applied in three split applications: 30 ml (second day after casing) + 15 ml (two weeks after casing) + 15 ml (after first flush, 20-25 days after casing). The biofungicide B. subtilis Ch-13 should be further investigated regarding its joint usage with chemical fungicides in different application procedures, as it showed remarkable characteristics both in terms of promoting mushroom yield and inhibiting the spread of mycopathogenic T. aggressivum.

Biocontrol Activity of Bacillus subtilis Isolated from Agaricus bisporus Mushroom Compost Against Pathogenic Fungi

2015 ◽  
Vol 63 (26) ◽  
pp. 6009-6018 ◽  
Author(s):  
Can Liu ◽  
Jiping Sheng ◽  
Lin Chen ◽  
Yanyan Zheng ◽  
David Yue Wei Lee ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Agaricus Bisporus ◽  
Pathogenic Fungi ◽  
Mushroom Compost ◽  
Biocontrol Activity

scholarly journals The First Report on Mushroom Green Mould Disease in Croatia / Prvi Izvještaj O Bolesti Zelene Plijesni U Hrvatskoj

2012 ◽  
Vol 63 (4) ◽  
pp. 481-487 ◽  
Author(s):  
Lóránt Hatvani ◽  
Petra Sabolić ◽  
Sándor Kocsubé ◽  
László Kredics ◽  
Dorina Czifra ◽  
...  
Keyword(s):  
Agaricus Bisporus ◽  
Optimal Growth ◽  
Oyster Mushroom ◽  
Temperature Profiles ◽  
Mushroom Compost ◽  
Severe Problem ◽  
First Report ◽  
Green Mould ◽  
Fungistatic Effect ◽  
Cultivated Mushrooms

AbstractGreen mould disease, caused by Trichoderma species, is a severe problem for mushroom growers worldwide, including Croatia. Trichoderma strains were isolated from green mould-affected Agaricus bisporus (button or common mushroom) compost and Pleurotus ostreatus (oyster mushroom) substrate samples collected from Croatian mushroom farms. The causal agents of green mould disease in the oyster mushroom were T. pleurotum and T. pleuroticola, similar to other countries. At the same time, the pathogen of A. bisporus was exclusively the species T. harzianum, which is different from earlier findings and indicates that the range of mushroom pathogens is widening. The temperature profiles of the isolates and their hosts overlapped, thus no range was found that would allow optimal growth of the mushrooms without mould contamination. Ferulic acid and certain phenolic compounds, such as thymol showed remarkable fungistatic effect on the Trichoderma isolates, but inhibited the host mushrooms as well. However, commercial fungicides prochloraz and carbendazim were effective agents for pest management. This is the first report on green mould disease of cultivated mushrooms in Croatia

Analysis of the activity and biological control efficacy of the Bacillus subtilis strain Bs-1 against Meloidogyne incognita

2019 ◽  
Vol 122 ◽  
pp. 125-135 ◽  
Author(s):  
Hongyi Cao ◽  
Yang Jiao ◽  
Nan Yin ◽  
Yan Li ◽  
Jian Ling ◽  
...  
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Meloidogyne Incognita ◽  
Subtilis Strain ◽  
Control Efficacy

scholarly journals The activity of native Bacillus subtilis strains in control of green mould disease of oyster mushroom (Pleurotus spp.)

2019 ◽  
Vol 34 (2) ◽  
pp. 97-102
Author(s):  
Ivana Potocnik ◽  
Svetlana Milijasevic-Marcic ◽  
Olja Stanojevic ◽  
Tanja Beric ◽  
Slavisa Stankovic ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Inhibition ◽  
Antagonistic Activity ◽  
Oyster Mushroom ◽  
Subtilis Strain ◽  
Mushroom Production ◽  
Antagonistic Microorganisms ◽  
Green Mould ◽  
Antagonistic Potential

The study aimed to isolate potential biocontrol agents from mushroom substrate that could serve as an alternative to toxic chemicals commonly used for disease control in mushroom production. The antagonistic potential of ten native Bacillus subtilis strains against the causal agents of green mould disease of oyster mushroom, Trichoderma pleuroti and Trichoderma pleuroticola, was evaluated. The antagonistic potential of Bacillus spp. strains was quantified in vitro based on dual cultivation with the pathogen. Growth inhibition of T. pleuroti ranged from 54.44% to 62.22% and no significant differences in antagonistic activity were found between the tested B. subtilis strains. Inhibition of T. pleuroticola was slightly higher, ranging from 55.56% to 69.62% and B. subtilis strain B-358 induced the highest growth inhibition. This research confirmed mushroom substrate to be a good source of antagonistic microorganisms with potentials for use in biological control of green mould in oyster mushroom production.

scholarly journals Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

2008 ◽  
Vol 65 (3) ◽  
pp. 565-573 ◽  
Author(s):  
Xianling Ji ◽  
Guobing Lu ◽  
Yingping Gai ◽  
Chengchao Zheng ◽  
Zhimei Mu
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Bacterial Wilt ◽  
Subtilis Strain

Biological control of green mould and dry bubble diseases of cultivated mushroom (Agaricus bisporus L.) by Bacillus spp.

2019 ◽  
Vol 126 ◽  
pp. 104944
Author(s):  
Olja Stanojević ◽  
Tanja Berić ◽  
Ivana Potočnik ◽  
Emil Rekanović ◽  
Slaviša Stanković ◽  
...  
Keyword(s):  
Biological Control ◽  
Agaricus Bisporus ◽  
Dry Bubble ◽  
Bacillus Spp ◽  
Cultivated Mushroom ◽  
Green Mould

scholarly journals Impact of Bacillus subtilis QST713 mushroom grain spawn treatment on yield and green mould control

2018 ◽  
Vol 33 (3-4) ◽  
pp. 205-211 ◽  
Author(s):  
Ivana Potocnik ◽  
Biljana Todorovic ◽  
Emil Rekanovic ◽  
Jelena Lukovic ◽  
Dusanka Paunovic ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Untreated Control ◽  
Agaricus Bisporus ◽  
Mycelial Growth ◽  
Casing Treatment ◽  
Mushroom Production ◽  
Green Mould

A biofungicide based on Bacillus subtilis QST713 was tested for impact on yield and efficacy against a Trichoderma aggressivum f. europaeum T77 strain from Serbia by coating mushroom grain spawn and comparing the results with the chemical fungicide prochloraz manganese in a mushroom growing room. The tested B. subtilis QST713 strain did not inhibit mycelial growth of Agaricus bisporus in plots free of the pathogen, showing an impact on yield of 91.95%, which was not significantly different from an untreated control. As for the efficacy of the fungicides used against T. aggressivum f. europaeum T77, there were no significant differences between a prochloraz manganese casing treatment, and B. subtilis QST713 coating on mushroom grain spawn, as the efficacy was 70.37 and 53.09%, respectively. These results implied that the biofungicide based on B. subtilis could serve as a harmless alternative to synthetic fungicides in mushroom production, especially during serious compost green muold outbreaks caused by T. aggressivum. Furthermore, the biofungicide should be applied alone because an antagonistic reaction was detected between the fungicide prochloraz and B. subtilis QST713.

Sign in / Sign up

Export Citation Format

Share Document