scholarly journals The activity of native Bacillus subtilis strains in control of green mould disease of oyster mushroom (Pleurotus spp.)

2019 ◽  
Vol 34 (2) ◽  
pp. 97-102
Author(s):  
Ivana Potocnik ◽  
Svetlana Milijasevic-Marcic ◽  
Olja Stanojevic ◽  
Tanja Beric ◽  
Slavisa Stankovic ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Inhibition ◽  
Antagonistic Activity ◽  
Oyster Mushroom ◽  
Subtilis Strain ◽  
Mushroom Production ◽  
Antagonistic Microorganisms ◽  
Green Mould ◽  
Antagonistic Potential

The study aimed to isolate potential biocontrol agents from mushroom substrate that could serve as an alternative to toxic chemicals commonly used for disease control in mushroom production. The antagonistic potential of ten native Bacillus subtilis strains against the causal agents of green mould disease of oyster mushroom, Trichoderma pleuroti and Trichoderma pleuroticola, was evaluated. The antagonistic potential of Bacillus spp. strains was quantified in vitro based on dual cultivation with the pathogen. Growth inhibition of T. pleuroti ranged from 54.44% to 62.22% and no significant differences in antagonistic activity were found between the tested B. subtilis strains. Inhibition of T. pleuroticola was slightly higher, ranging from 55.56% to 69.62% and B. subtilis strain B-358 induced the highest growth inhibition. This research confirmed mushroom substrate to be a good source of antagonistic microorganisms with potentials for use in biological control of green mould in oyster mushroom production.

scholarly journals Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper

2018 ◽  
Vol 33 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Svetlana Milijasevic-Marcic ◽  
Vesna Todorovic ◽  
Olja Stanojevic ◽  
Tanja Beric ◽  
Slavisa Stankovic ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Antagonistic Effect ◽  
Subtilis Strain ◽  
Vegetable Crops ◽  
Clavibacter Michiganensis Subsp ◽  
Bacillus Spp ◽  
Antagonistic Potential

In vitro antagonistic potential of eleven isolates of Bacillus spp. against two phytopathogenic bacteria and one fungus was tested in order to identify potential biocontrol agents in vegetable crops. The Bacillus spp. isolates demonstrated different levels of antagonistic effect against the tested pathogenic microorganisms. Data in the study proved Xanthomonas vesicatoria to be more sensitive to Bacillus spp. strains than Clavibacter michiganensis subsp. michiganensis. Ten B. subtilis strains induced growth inhibition of X. vesicatoria, while a strain of B. pumilus did not affect the growth of that bacterium. The largest inhibition zones against X. vesicatoria were induced by strains B-319, B-325 and B-358. The pathogenic strain C. michiganensis subsp. michiganensis was most inhibited by two B. subtilis strains (B-338 and B-348) with mean inhibition zone diameters of up to 20 mm. B. subtilis strain B-319 which was the best in inhibiting X. vesicatoria, showed the lowest inhibitory effect on C. michiganensis subsp. michiganensis. The largest growth inhibition percentage of Verticillium sp. (PGI approximately 70%) was induced by B. subtilis strains B-310 and B-322. The other B. subtilis strains showed PGI values ranging from 45% to 68%, while B. pumilus strain B-335 had the least antagonistic potential (PGI =34.43%) against the pathogen. This study identified at least one suitable biocontrol candidate, B. subtilis strain B-358, as effective in vitro against all three vegetable pathogens.

scholarly journals Biocontrol potential of Streptomyces sp. CACIS-1.5CA against phytopathogenic fungi causing postharvest fruit diseases

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Zahaed Evangelista-Martínez ◽  
Erika Anahí Contreras-Leal ◽  
Luis Fernando Corona-Pedraza ◽  
Élida Gastélum-Martínez
Keyword(s):  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Fruit Rot ◽  
Main Body ◽  
Type I ◽  
Tropical Fruit ◽  
Habanero Pepper ◽  
Antagonistic Microorganisms

Abstract Background Fungi are one of the microorganisms that cause most damage to fruits worldwide, affecting their quality and consumption. Chemical controls with pesticides are used to diminish postharvest losses of fruits. However, biological control with microorganisms or natural compounds is an increasing alternative to protect fruits and vegetables. In this study, the antifungal effect of Streptomyces sp. CACIS-1.5CA on phytopathogenic fungi that cause postharvest tropical fruit rot was investigated. Main body Antagonistic activity was evaluated in vitro by the dual confrontation over fungal isolates obtained from grape, mango, tomato, habanero pepper, papaya, sweet orange, and banana. The results showed that antagonistic activity of the isolate CACIS-1.5CA was similar to the commercial strain Streptomyces lydicus WYEC 108 against the pathogenic fungi Colletotrichum sp., Alternaria sp., Aspergillus sp., Botrytis sp., Rhizoctonia sp., and Rhizopus sp. with percentages ranging from 30 to 63%. The bioactive extract obtained from CACIS-1.5 showed a strong inhibition of fungal spore germination, with percentages ranging from 92 to 100%. Morphological effects as irregular membrane border, deformation, shrinkage, and collapsed conidia were observed on the conidia. Molecularly, the biosynthetic clusters of genes for the polyketide synthase (PKS) type I, PKS type II, and NRPS were detected in the genome of Streptomyces sp. CACIS-1.5CA. Conclusions This study presented a novel Streptomyces strain as a natural alternative to the use of synthetic fungicides or other commercial products having antagonistic microorganisms that were used in the postharvest control of phytopathogenic fungi affecting fruits.

A Bacillus subtilis strain as probiotic in poultry: selection based on in vitro functional properties and enzymatic potentialities

2017 ◽  
Vol 44 (8) ◽  
pp. 1157-1166 ◽  
Author(s):  
Houda Hmani ◽  
Lobna Daoud ◽  
Mouna Jlidi ◽  
Karim Jalleli ◽  
Manel Ben Ali ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Functional Properties ◽  
Subtilis Strain

scholarly journals Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro

2005 ◽  
Vol 160 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Bhaskar Chaurasia ◽  
Anita Pandey ◽  
Lok Man S. Palni ◽  
Pankaj Trivedi ◽  
Bhavesh Kumar ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Volatile Compounds ◽  
Pathogenic Fungi ◽  
Subtilis Strain ◽  
Structural Deformations

Characterization and sequence analysis of potential biofertilizer and biocontrol agent Bacillus subtilis strain SEM-9 from silkworm excrement

2019 ◽  
Vol 65 (1) ◽  
pp. 45-58 ◽  
Author(s):  
Qingrong Li ◽  
Sentai Liao ◽  
Huyu Zhi ◽  
Dongxu Xing ◽  
Yang Xiao ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Gene Clusters ◽  
Antagonistic Effect ◽  
Subtilis Strain ◽  
Fusarium Spp ◽  
Antagonistic Microorganisms ◽  
Biochemical Analyses ◽  
Physiological And Biochemical

Fusarium wilt is a devastating soil-borne disease caused mainly by highly host-specific formae speciales of Fusarium oxysporum. Antagonistic microorganisms play a very important role in Fusarium wilt control, and the isolation of potential biocontrol strains is becoming more and more important. We isolated a bacterial strain (SEM-9) from the high-temperature stage of silkworm excrement composting, which had a marked ability to solubilize phosphorus, promote the growth and increase the yield of the small Chinese cabbage, and which also exhibited considerable antagonistic effect towards Fusarium sambucinum and other fungi. The result of physiological and biochemical analyses, as well as genome sequencing, showed that SEM-9 was a strain of Bacillus subtilis. Through genome annotation and analysis, it was found that SEM-9 contained genes related to the regulation of biofilm formation, which may play an important role in colonization, and gene clusters encoding the biosynthesis of antimicrobials, such as surfactin, bacilysin, fengycin, and subtilosin-A. The production of such antifungal compounds may constitute the basis of the mode-of-action of SEM-9 against Fusarium spp. These data suggested that the SEM-9 strain has potential as both a biofertilizer and a biocontrol agent, with the potential to manage Fusarium wilt disease in crops.

Characterization of an antifungal soil bacterium and its antagonistic activities againstFusariumspecies

2003 ◽  
Vol 49 (4) ◽  
pp. 253-262 ◽  
Author(s):  
Yiu-Kwok Chan ◽  
Wayne A McCormick ◽  
Keith A Seifert
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Fusarium Species ◽  
Hyphal Growth ◽  
Antagonistic Activity ◽  
Plant Diseases ◽  
Ear Rot ◽  
In Planta ◽  
Head Blight

Bacteria were isolated from a cultivated soil and screened for antagonistic activity against Fusarium graminearum, a predominant agent of ear rot and head blight in cereal crops. Based on its in vitro effectiveness, isolate D1/2 was selected for characterization and identified as a strain of Bacillus subtilis by phenotypic tests and comparative analysis of its 16S ribosomal RNA gene (rDNA) sequence. It inhibited the mycelial growth of a collection of common fungal phytopathogens, including eight Fusarium species, three other ascomycetes, and one basidiomycete. The cell-free culture filtrate of D1/2 at different dilutions was active against macroconidium germination and hyphal growth of F. graminearum, depending on the initial macroconidium density. It induced the formation of swollen hyphal cells in liquid cultures of this fungus grown from macroconidia. A bioassay also demonstrated that D1/2 offered in planta protection against the damping-off disease in alfalfa seedlings caused by F. graminearum, while the type strain of B. subtilis was ineffective. Hence, B. subtilis D1/2 or its culture filtrate has potential application in controlling plant diseases caused by Fusarium.Key words: antifungal activity, Bacillus subtilis, biological control, biopesticide, Fusarium species.

In vitro Antagonistic Activity of Diverse Bacillus Species Against Cochliobolus sativus (Common Root Rot) of Barley

2020 ◽  
Vol 55 (1) ◽  
pp. 35-42
Author(s):  
M. Harba ◽  
M. Jawhar ◽  
M. I. E. Arabi
Keyword(s):  
16S Rrna Gene Sequencing ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Cochliobolus Sativus ◽  
Bacillus Species ◽  
Rrna Gene ◽  
Bacillus Sp ◽  
Common Root ◽  
Antagonistic Microorganisms

Antagonistic microorganisms have been used as biological control agents to reduce the use of chemical fungicides in the control of crop diseases. The present work was conducted to determine the antagonistic potential of Bacillus sp. isolates against the soil-borne fungus Cochliobolus sativus, the causal agent of barley common root (CRR) disease. Out of 525 isolates, 40 were showed in vitro antagonistic activity against the virulent C. sativus isolate CRR16. On the basis of 16S rRNA gene sequencing Bacillus sp. isolates are identified as B. atrophaeus, B. subtilis, Paenibacillus polymyxa, B. amyloliquefaciens, B. simplex and B. tequilensis. Results showed that Bacillus sp. had significant (P<0.05) antagonistic activities against the C. sativus where the percentage of radial growth inhibition of the fungi colonies ranged from 59 to 92%, compared to the untreated control. The B. subtilis isolate SY41B had the highest inhibition effect on the vegetative growth zones as compared with the other species isolates. In view of these, our results indicate that the antagonistic effect of the Bacillus sp. isolates may be important contributors as a biocontrol approach that could be employed as a part of integrated CRR management system.

scholarly journals Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

2017 ◽  
Vol 53 (No. 3) ◽  
pp. 134-143 ◽  
Author(s):  
Mohammadi Parisa ◽  
Tozlu Elif ◽  
Kotan Recep ◽  
Kotan Merve Şenol
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Mycelial Growth ◽  
Spore Production ◽  
Penicillium Digitatum ◽  
Broth Culture ◽  
Agrobacterium Radiobacter ◽  
Green Mould

Ten bacteria isolate (4 Bacillus subtilis, 2 Bacillus pumilus, 2 Bacillus cereus, 1 Bacillus megaterium, and 1 Agrobacterium radiobacter) were tested in vitro for antagonistic properties against Penicillium digitatum, the causal agent of citrus green mould. The effect of these bacteria was also observed on mycelial growth, spore germination, and spore production of the pathogenic fungus in broth culture. Extracellular enzyme activities of the bacteria were determined. According to the results of in vitro antagonistic tests and enzymes activities, the most promising bacteria were Bacillus subtilis and Agrobacterium radiobacter. These bacteria were tested for disease suppression on lemon fruits. In addition, these bacterial isolates also showed remarkable antifungal activity against the pathogen on lemon fruits. The results of this study showed that Bacillus subtilis and Agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Chitinase and glucanase enzyme activity of all the tested bacteria was positive. Protease enzyme activity was positive in all tested bacteria with the exception of Agrobacterium radiobacter. In addition, all bacteria inhibited mycelial growth and spore germination (except Agrobacterium radiobacter) of the fungus. Bacillus subtilis, Bacillus cereus, and Agrobacterium radiobacter inhibited spore production in broth culture. Bacillus subtilis and Agrobacterium radiobacter were tested on lemon fruits significantly reduced disease severity. Consequently, these isolates can be used as new biocontrol agents in controlling the post-harvest decay of citrus fruits caused by Penicillium digitatum.

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