Biological control of yellow rust of wheat ( Puccinia striiformis ) with Serenade ® ASO ( Bacillus subtilis strain QST713)

Two mutants were isolated in wheat that showed enhanced resistance towards Puccinia striiformis f. sp. tritici, the fungal causal agent of yellow rust. The altered phenotype of I3-48 is due to a minimum of two mutation events, each showing a partial, additive effect, with one mutation segregating with a deletion on the long arm of chromosome 4D. In the case of I3-54, the enhanced resistance is due to a single, dominant mutation. In both mutants, the expression of the enhanced resistance is growth-stage specific. With I3-54, the full resistance phenotype is apparent from the third seedling leaf onwards, while with I3-48, a full resistance phenotype is only seen on the tenth and subsequent leaves. In addition to the enhanced resistance towards yellow rust, I3-48 also shows enhanced resistance towards brown rust, and I3-54 shows enhanced resistance to powdery mildew.

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Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1

Canadian Journal of Microbiology ◽

10.1139/m96-072 ◽

1996 ◽

Vol 42 (6) ◽

pp. 533-538 ◽

Cited By ~ 64

Author(s):

A. R. Podile ◽

A. P. Prakash

Keyword(s):

Biological Control ◽

Bacillus Subtilis ◽

Aspergillus Niger ◽

Fungal Growth ◽

Dry Weight ◽

Crown Rot ◽

Fungal Mycelium ◽

Infested Soil ◽

Dual Culture ◽

Bacterial Cells

A biocontrol rhizobacterial strain of Bacillus subtilis AF 1 grown for 6 h was coinoculated with Aspergillus niger at different time intervals and microscopic observations revealed adherence of bacterial cells to the fungal mycelium. Bacterial cells multiplied in situ and colonized the mycelial surface. Growth of AF 1 resulted in damage to the cell wall, followed by lysis. AF 1 inoculation into media containing A. niger at 0, 6, and 12 h suppressed >90% fungal growth, while in 18- and 24-h cultures fungal growth inhibition was 70 and 56%, respectively, in terms of dry weight. In dual culture the fungal growth was not accompanied by formation of spores. The mycelial preparation of A. niger as principal carbon source supported the growth of B. subtilis, as much as chitin. Extracellular protein precipitate from B. subtilis culture filtrate had a significant growth-retarding effect on A. niger. Groundnut seeds bacterized with B. subtilis showed a reduced incidence of crown rot in A. niger infested soil, suggesting a possible role of B. subtilis in biological control of A. niger.Key words: mycolytic bacteria, Bacillus subtilis, Aspergillus niger, biological control.

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Cloning, sequencing and expression of the xylanase gene from a Bacillus subtilis strain B10 in Escherichia coli

Bioresource Technology ◽

10.1016/j.biortech.2005.04.011 ◽

2006 ◽

Vol 97 (6) ◽

pp. 802-808 ◽

Cited By ~ 38

Author(s):

Junli Huang ◽

Guixue Wang ◽

Li Xiao

Keyword(s):

Escherichia Coli ◽

Bacillus Subtilis ◽

Subtilis Strain ◽

Xylanase Gene ◽

Sequencing And Expression

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Complete Genome Sequence of Bacillus subtilis subsp. subtilis Strain ∆6

Genome Announcements ◽

10.1128/genomea.00759-16 ◽

2016 ◽

Vol 4 (4) ◽

Cited By ~ 3

Author(s):

Daniel R. Reuß ◽

Andrea Thürmer ◽

Rolf Daniel ◽

Wim J. Quax ◽

Jörg Stülke

Keyword(s):

Bacillus Subtilis ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Full Genome Sequence ◽

Subtilis Strain ◽

Full Genome ◽

Biotechnological Applications ◽

A Genome ◽

Subtilis Subsp

Bacillus subtilis ∆6 is a genome-reduced strain that was cured from six prophages and AT-rich islands. This strain is of great interest for biotechnological applications. Here, we announce the full-genome sequence of this strain. Interestingly, the conjugative element ICE Bs 1 has most likely undergone self-excision in B. subtilis ∆6.

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