Genetic diversity of resynthesized Brassica napus lines from SW China assessed by main agronomic traits and SSR markers in comparison with common B. napus lines

Genetic diversity and relationship of 92 bread wheat (Triticum aestivum L.) genotypes from India and exotic collections were examined using simple sequence repeat (SSR) markers and phenotypic traits to identify new sources of diversity that could accelerate the development of improved wheat varieties better suited to meet the challenges posed by heat stress in India. Genetic diversity assessed by using 82 SSR markers was compared with diversity evaluated using five physiological and six agronomic traits under the heat stress condition. A total of 248 alleles were detected, with a range of two to eight alleles per locus. The average polymorphic information content value was 0.37, with a range of 0.04 (cfd9) to 0.68 (wmc339). The heat susceptibility index was determined for grain yield per spike, and the genotypes were grouped into four categories. Two dendrograms that were constructed based on phenotypic and molecular analysis using UPGMA (unweighted pair group method with arithmetic mean) were found to be topologically different. Genotypes characterized as highly heat tolerant were distributed among all the SSR-based cluster groups. This implies that the genetic basis of heat stress tolerance in these genotypes is different, thereby enabling wheat breeders to combine these diverse sources of genetic variability to improve heat tolerance in their breeding programmes.

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Genetic diversity and relationship analysis of the Brassica napus germplasm using simple sequence repeat (SSR) markers

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.3475 ◽

2012 ◽

Vol 11 (27) ◽

Cited By ~ 1

Author(s):

Cunmin Qu

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Ssr Markers ◽

Simple Sequence Repeat ◽

Sequence Repeat ◽

Relationship Analysis ◽

Simple Sequence

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Genetic Diversity of Shanlan Upland Rice (Oryza sativa L.) and Association Analysis of SSR Markers Linked to Agronomic Traits

BioMed Research International ◽

10.1155/2021/7588652 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Guofeng Yang ◽

Yong Yang ◽

Yali Guan ◽

Zhixia Xu ◽

Junyu Wang ◽

...

Keyword(s):

Genetic Diversity ◽

Plant Height ◽

Ssr Markers ◽

Agronomic Traits ◽

Upland Rice ◽

Diversity Index ◽

Oryza Sativa L ◽

Grain Number ◽

Rice Germplasm ◽

Panicle Number

Shanlan upland rice, a kind of unique rice germplasm in Hainan Island, was used to evaluate genetic diversity and association between SSR markers and agronomic traits. A total of 239 alleles were detected in 57 Hainan upland rice varieties using 35 SSR markers, and the number of alleles per locus was 2-19. The observed heterozygosity was 0.0655-0.3115. The Shannon diversity index was 0.1352-0.4827. The genetic similarity coefficient was 0.6736-0.9707, and 46 varieties were clustered into one group, indicating that the genetic base of the Shanlan upland rice germplasm was narrow. A total of 25 SSR markers significantly related to plant height, effective panicle number per plant, panicle length, total grain number, filled grain number, seed rating rate, and 1000-grain weight were obtained ( P < 0.01 ), with the percentage of the total variations explained ranging from 0.12% to 42.62%. RM208 explained 42.62% of the total variations in plant height of Shanlan upland rice. RM493 was significantly associated with 6 agronomic traits. We can speculate that RM208 may flank QTLs responsible for plant height and RM493 may flank QTLs playing a fundamental role in the intertwined regulatory network of agronomic traits of Shanlan upland rice.

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