Extending the rapeseed genepool with resynthesized Brassica napus L. I: Genetic diversity

In cultivated commercial crop species, genetic diversity tends to decrease because of the extensive breeding processes. Therefore, germplasm of commercial crop species, such as Brassica napus L. should be evaluated and the genotypes, which have higher genetic diversity index, should be addressed as potential parental cross materials in breeding programs. In this study, the genetic diversity was analysed by using randomly amplified polymorphic DNA analysis (RAPD) technique in nine Turkish commercial rapeseed varieties. The RAPD primers (10-mer oligonucleotides) produced 51 scorable loci, 31 loci of which were polymorphic (60.78%) and 20 loci (39.22%) were monomorphic The RAPD bands were scored as binary matrix data and were analysed using POPGENE version 1.32. At locus level, the values of genetic diversity within population (Hs) and total (HT) were 0.15 and 0.19 respectively. The genetic differentiation (GST) and the gene flow (Nm) values between the populations were 0.20 and 2.05 respectively. The mean number of alleles (na), the mean number of effective alleles (nae), and the mean value of genetic diversity (He) were 2.00, 1.26, and 0.19 respectively. According to Pearson’s correlation, multiple regression and principal component analyses, eco-geographical conditions in combination had significant effect on genetic indices of commercial B. napus L. varieties were discussed.

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Genetic diversity assessment in winter oilseed rape (Brassica napus L.) collection using AFLP, ISSR and SSR markers

Czech Journal of Genetics and Plant Breeding ◽

10.17221/220/2013-cjgpb ◽

2014 ◽

Vol 50 (No. 3) ◽

pp. 216-225 ◽

Cited By ~ 3

Author(s):

L. Havlíčková ◽

E. Jozová ◽

A. Rychlá ◽

M. Klíma ◽

V. Kučera ◽

...

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Oilseed Rape ◽

Issr Markers ◽

Appropriate Technology ◽

Winter Oilseed Rape ◽

Brassica Napus L ◽

High Genetic Diversity ◽

Diversity Assessment ◽

Breeding Programmes

The genetic diversity of 94 accessions of winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs and AFLPs. All three kinds of markers differed in the range of observed polymorphism and differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP and 53 ISSR markers were evaluated. Their average rates of polymorphic bands were 100%, 53.9% and 90.6%, respectively, indicating high genetic diversity among the accessions. The greatest genetic distance was found by ISSRs (62.3%) whereas significantly lower distances of 49.4% in SSRs and 35.5% in AFLPs were observed. The genetic similarity matrix clearly distinguished all accessions. A set of the most distinct varieties was established. The analysis of the genetic pattern of the accessions indicated two groups comprising most of the modern Czech breeding materials, revealing a distinct shift in breeding. Surprisingly, molecular analyses did not support  breeders’ views about a narrow genetic base of the Czech breeding materials. The choice of appropriate technology for different aspects of germplasm evaluation is also discussed.

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Genetic diversity analysis in f5 population of Brassica napus L.

International Journal of Biosciences (IJB) ◽

10.12692/ijb/9.3.177-184 ◽

2016 ◽

Vol 9 (3) ◽

pp. 177-184

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Diversity Analysis ◽

Brassica Napus L ◽

Genetic Diversity Analysis

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Genetic Diversity of Chinese and Japanese Rapeseed (Brassica napus L.) Varieties Detected by RAPD Markers.

Breeding Science ◽

10.1270/jsbbs.50.257 ◽

2000 ◽

Vol 50 (4) ◽

pp. 257-265 ◽

Cited By ~ 10

Author(s):

Chaozhi Ma ◽

Yusuke Kimura ◽

Hideya Fujimoto ◽

Takako Sakai ◽

Jun Imamura ◽

...

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Rapd Markers ◽

Brassica Napus L

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Assessment of Genetic Diversity of 62 F4 Population of Rapeseed (Brassica Napus L.) Through Multivariate Analysis

SAARC Journal of Agriculture ◽

10.3329/sja.v19i1.54775 ◽

2021 ◽

Vol 19 (1) ◽

pp. 15-25

Author(s):

S Sultana ◽

F Mahmud ◽

M Asaduzzaman

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Multivariate Analyses ◽

Wide Spectrum ◽

Block Design ◽

Agronomic Performance ◽

Brassica Napus L ◽

Randomized Complete Block Design ◽

Cluster Distance ◽

Segregating Population

Rapeseed (Brassica napus L.) is the best one in respect of oil production. It is the order of the day to take better steps for production and quality improvement of our local cultivars. Broadening of genetic diversity in spring oilseed Brassica napus L. (AACC, 2n = 38), canola is important for continued improvement of this crop. Sixty-two genotypes in F4 generation of Brassica napus L. were evaluated to assess genetic diversity based on randomized complete block design with three replications at the experimental field of Sher-e-Bangla Agricultural University, Dhaka. Different Multivariate analyses were performed to classify 62 genotypes. On the basis of cluster analysis, all the genotypes were classified in five clusters. The cluster IV comprised the maximum number (19) of genotypes followed by same in cluster II (18). The cluster I and V comprised 10 and 9 genotypes respectively. The lowest number of genotypes was present in cluster II. The highest inter-cluster distance (10.309) was observed between the cluster I and IV and the genotypes of these clusters involved in hybridization may produce a wide spectrum of segregating population. The lowest inter-cluster distance (3.513) was observed between the cluster III and IV. The inter-cluster distances were larger than the intra-cluster distances. Considering cluster distance, inter genotypic distance and other agronomic performance G3, G4, G24, G35 and G51 might be suggested for future breeding program. SAARC J. Agric., 19(1): 15-25 (2021)

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GENETIC DIVERSITY OF RAPESEED (Brassica napus L.) GENOTYPES IN BANGLADESH

Bangladesh Journal of Plant Breeding and Genetics ◽

10.3329/bjpbg.v30i2.37340 ◽

2017 ◽

Vol 30 (2) ◽

pp. 29-34

Author(s):

S. D. S. D. Joya ◽

A. K. M. Shamsuddin ◽

U. K. Nath

Keyword(s):

Genetic Diversity ◽

Brassica Napus ◽

Harvest Index ◽

High Yield ◽

Brassica Napus L ◽

Large Size ◽

Parental Material ◽

Number Of Seeds ◽

High Seed Yield ◽

Selection Of

Genetic diversity in thirty eight traditional rapeseed genotypes was studied under favorable condition through Mahalanobis D2 statistic for yield and yield contributing characters. The genotypes were grouped into five clusters. The inter-cluster distances were higher than intracluster distances indicating wider genetic diversity among the clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the clusters. Among the different cluster the genotypes of the cluster IV, III and I included were taller plant. The genotypes in the cluster III and IV had large size raceme. The genotypes in the cluster IV, III and V exhibited comparatively higher number of siliqua per raceme. Longer siliqua was noticed for the genotypes in the cluster III, IV and I. Higher number of seeds per siliqua noticed in clusters I, II and III. The genotypes of the cluster I and II produced bold size seed. The genotypes in the cluster V and I had high harvest index. The genotypes of the cluster III and I produced high seed yield per plant. Among the different cluster, the cluster III included the genotypes which had high yield, higher number of seeds per siliqua, longer siliqua, siliqua number per raceme and high plant height. Moreover these cluster displayed wide divergence with the genotypes of cluster V. The genotypes of the cluster V had the highest harvest index therefore selection of the parental material for crossing program for improvement of yield in rapeseed is suggested from these two clusters.

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