The antioxidant system of seminal fluid during in vitro storage of sterlet Acipenser ruthenus sperm

2015 ◽  
Vol 42 (2) ◽  
pp. 563-568 ◽  
Author(s):  
Viktoriya Dzyuba ◽  
Jacky Cosson ◽  
Borys Dzyuba ◽  
Gunes Yamaner ◽  
Marek Rodina ◽  
...  
Keyword(s):  
Antioxidant System ◽  
Seminal Fluid ◽  
In Vitro Storage ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

scholarly journals Influence of Environmental Temperature and Hormonal Stimulation on the In Vitro Sperm Maturation in Sterlet Acipenser ruthenus in Advance of the Spawning Season

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1417
Author(s):  
Viktoriya Dzyuba ◽  
Jacky Cosson ◽  
Maria Papadaki ◽  
Constantinos C. Mylonas ◽  
Christoph Steinbach ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Environmental Temperature ◽  
Spawning Season ◽  
Sperm Maturation ◽  
Hormonal Stimulation ◽  
Acipenser Ruthenus ◽  
Hormonal Induction ◽  
Sterlet Acipenser Ruthenus ◽  
Stimulation Of

Sturgeon sperm maturation occurs outside the testes during the transit of testicular spermatozoa (TS) through the kidneys and the Wolffian ducts. A method of in vitro TS maturation in sterlet Acipenser ruthenus was used to investigate the effects of temperature and hormonal stimulation of spermiation on the ability of TS to complete this process. Spermatozoa motility parameters after in vitro maturation of testicular sperm, concentrations of sex steroid hormones and testis morphology were studied in three groups of sterlet: (1) after overwintering in ponds (OW), (2) adapted to spawning temperature (ST), and (3) adapted to spawning temperature with hormonal induction of spermiation (ST-HI). Blood plasma concentrations of testosterone, 11-ketotestosterone and 17,20β-dihydroxy-pregnenolone increased significantly after hormonal induction of spermiation (group ST-HI). In all groups, TS were not motile. After in vitro sperm maturation, motility was up to 60% only in group ST-HI. The data suggest that the ability of TS to be matured in vitro was not related to the environmental temperature, while hormonal stimulation of spermiation during the spawning season was an absolute requirement for optimal in vitro maturation.

In vitro sperm maturation in sterlet, Acipenser ruthenus

2014 ◽  
Vol 14 (2) ◽  
pp. 160-163 ◽  
Author(s):  
Borys Dzyuba ◽  
Jacky Cosson ◽  
Sergii Boryshpolets ◽  
Olga Bondarenko ◽  
Viktoriya Dzyuba ◽  
...  
Keyword(s):  
Sperm Maturation ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus

In vitro oocyte maturation and blood‐circulating steroid hormones in Sterlet ( Acipenser ruthenus ) and Siberian sturgeon ( Acipenser baeri )

2020 ◽  
Vol 51 (9) ◽  
pp. 3488-3494
Author(s):  
Hajar Azarin ◽  
Mohammad Reza Imanpour ◽  
Atefeh Karamad ◽  
Ahmad Reza Jebeleh ◽  
Sajjad Pourmozaffar
Keyword(s):  
Steroid Hormones ◽  
Oocyte Maturation ◽  
Siberian Sturgeon ◽  
Acipenser Baeri ◽  
Acipenser Ruthenus ◽  
In Vitro Oocyte Maturation ◽  
Sterlet Acipenser Ruthenus
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