Kiwi fruit PMEI inhibits PME activity, modulates root elongation and induces pollen tube burst in Arabidopsis thaliana

2014 ◽  
Vol 74 (3) ◽  
pp. 285-297 ◽  
Author(s):  
Florence Paynel ◽  
Christelle Leroux ◽  
Ogier Surcouf ◽  
Annick Schaumann ◽  
Jérôme Pelloux ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Root Elongation ◽  
Kiwi Fruit

scholarly journals Two Expansin Genes, AtEXPA4 and AtEXPB5, Are Redundantly Required for Pollen Tube Growth and AtEXPA4 Is Involved in Primary Root Elongation in Arabidopsis thaliana

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 249
Author(s):  
Weimiao Liu ◽  
Liai Xu ◽  
Hui Lin ◽  
Jiashu Cao
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Cell Walls ◽  
Root Elongation ◽  
Expression Patterns ◽  
Primary Root ◽  
Pollen Grains ◽  
Tube Growth ◽  
Cell Wall Binding

The growth of plant cells is inseparable from relaxation and expansion of cell walls. Expansins are a class of cell wall binding proteins, which play important roles in the relaxation of cell walls. Although there are many members in expansin gene family, the functions of most expansin genes in plant growth and development are still poorly understood. In this study, the functions of two expansin genes, AtEXPA4 and AtEXPB5 were characterized in Arabidopsis thaliana. AtEXPA4 and AtEXPB5 displayed consistent expression patterns in mature pollen grains and pollen tubes, but AtEXPA4 also showed a high expression level in primary roots. Two single mutants, atexpa4 and atexpb5, showed normal reproductive development, whereas atexpa4atexpb5 double mutant was defective in pollen tube growth. Moreover, AtEXPA4 overexpression enhanced primary root elongation, on the contrary, knocking out AtEXPA4 made the growth of primary root slower. Our results indicated that AtEXPA4 and AtEXPB5 were redundantly involved in pollen tube growth and AtEXPA4 was required for primary root elongation.

scholarly journals Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kazuki Motomura ◽  
Hidenori Takeuchi ◽  
Michitaka Notaguchi ◽  
Haruna Tsuchi ◽  
Atsushi Takeda ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Sperm Cell ◽  
Pollen Tubes ◽  
Vegetative Nucleus ◽  
Sperm Cells ◽  
Apical Region ◽  
Basal Region ◽  
Specific Expression ◽  
Directional Growth

AbstractDuring the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.

scholarly journals Termination mechanism of the pollen tube attraction in Arabidopsis thaliana

2016 ◽  
Vol 28 (1) ◽  
pp. 43-47
Author(s):  
Daisuke Maruyama ◽  
Tetsuya Higashiyama
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube

scholarly journals Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube

Plants ◽  
2013 ◽  
Vol 2 (3) ◽  
pp. 429-440 ◽  
Author(s):  
Mário da Costa ◽  
Luís Pereira ◽  
Sílvia Coimbra
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Pollen Tube ◽  
Growth Media

scholarly journals The Kinase ERULUS Controls Pollen Tube Targeting and Growth in Arabidopsis thaliana

2017 ◽  
Vol 8 ◽  
Author(s):  
Sébastjen Schoenaers ◽  
Daria Balcerowicz ◽  
Alex Costa ◽  
Kris Vissenberg
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube

scholarly journals Transcriptome and translatome changes in germinated pollen under heat stress uncover roles of transporter genes involved in pollen tube growth

2020 ◽  
Author(s):  
Laetitia Poidevin ◽  
Javier Forment ◽  
Dilek Unal ◽  
Alejandro Ferrando
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Stress ◽  
High Temperature ◽  
Heat Shock ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Heat Shock Genes ◽  
Germinated Pollen

ABSTRACTPlant reproduction is one key biological process very sensitive to heat stress and, as a consequence, enhanced global warming poses serious threats to food security worldwide. In this work we have used a high-resolution ribosome profiling technology to study how heat affects both the transcriptome and the translatome of Arabidopsis thaliana pollen germinated in vitro. Overall, a high correlation between transcriptional and translational responses to high temperature was found, but specific regulations at the translational level were also present. We show that bona fide heat shock genes are induced by high temperature indicating that in vitro germinated pollen is a suitable system to understand the molecular basis of heat responses. Concurrently heat induced significant down-regulation of key membrane transporters required for pollen tube growth, thus uncovering heat-sensitive targets. We also found that a large subset of the heat-repressed transporters is specifically up-regulated, in a coordinated manner, with canonical heat-shock genes in pollen tubes grown in vitro and semi in vivo, based on published transcriptomes from Arabidopsis thaliana. Ribosome footprints were also detected in gene sequences annotated as non-coding, highlighting the potential for novel translatable genes and translational dynamics.

scholarly journals Changes in cell ultrastructure and morphology of Arabidopsis thaliana roots after coumarins treatment

2014 ◽  
Vol 70 (3) ◽  
pp. 187-198
Author(s):  
Ewa Kupidłowska
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Walls ◽  
Root Elongation ◽  
Inhibitory Effect ◽  
Cell Ultrastructure ◽  
Radial Expansion ◽  
Elongation Zone ◽  
Cortical Cells ◽  
Mother Cell Wall

The ultrastructure and morphology of roots treated with coumarin and umbelliferone as well as the reversibility of the coumarins effects caused by exogenous GA, were studied in <em>Arabidopsis thaliana</em>. Both coumarins suppressed root elongation and appreciably stimulated radial expansion of epidermal and cortical cells in the upper part of the meristem and in the elongation zone. The gibberellic acid applied simultaneously with coumarins decreased their inhibitory effect on root elongation and reduced cells swelling.Microscopic observation showed intensive vacuolization of cells and abnormalities in the structure of the Golgi stacks and the nuclear envelope. The detection of active acid phosphatase in the cytosol of swollen cells indicated increased membrane permeability. Significant abnormalities of newly formed cell walls, e.g. the discontinuity of cellulose layer, uncorrect position of walls and the lack of their bonds with the mother cell wall suggest that coumarins affected the cytoskeleton.

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