Integrated physiological, metabolomic and transcriptomic analyses provide insights into the roles of exogenous melatonin in promoting rice seed germination under salt stress

2021 ◽  
Author(s):  
Liexiang Huangfu ◽  
Zihui Zhang ◽  
Yong Zhou ◽  
Enying Zhang ◽  
Rujia Chen ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Rice Seed ◽  
Exogenous Melatonin

Exogenous melatonin promotes rice seed germination under salinity through regulating antioxidants and metabolic homeostasis

2020 ◽  
Author(s):  
Liexiang Huangfu ◽  
Enying Zhang ◽  
Huimin Fang ◽  
Pengcheng Li ◽  
Yang Xu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Stress Responses ◽  
Molecular Mechanisms ◽  
Main Body ◽  
Rice Seed ◽  
Metabolic Homeostasis ◽  
Exogenous Melatonin ◽  
Total Antioxidant ◽  
Pre Treatment

Abstract Background Melatonin plays important roles in multiple plant developmental processes and stress responses. However, little is known about the role and putative mechanism of exogenous melatonin in regulating rice seed germination under salt stress. Main Body Here, we revealed that the exogenous application of melatonin can significantly promote rice seed germination under salinity. Its putative molecular mechanisms are further investigated through metabolomic and transcriptomic analyses. The results revealed that the phytohormone concentrations in germinating seeds are reprogrammed, the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) are enhanced, and the total antioxidant capacity under salinity is activated by exogenous melatonin. In addition, rice seeds pre-treated with melatonin exhibit higher concentrations of glycosides than non-treated seeds under salinity. Furthermore, exogenous melatonin alleviates the accumulation of fatty acids under salinity. Genome-wide transcriptomic profiling is used to identify 7160 transcripts which are differentially expressed under salt (NaCl), salt + melatonin (MT100), and control treatments. Pathway and GO enrichment analyses reveal that the genes involved in the response to oxidative stress, hormone metabolism, heme building, mitochondrion, and tricarboxylic acid transformation are altered after melatonin pre-treatment under salinity. Conclusion This study provides evidence for exogenous melatonin increasing rice seed germination under salt stress, mainly through the activation of antioxidants and modulation of metabolic homeostasis.

Proteome Analysis of Relieving Effect of Gibberellin on the Inhibition of Rice Seed Germination by Salt Stress

2009 ◽  
Vol 35 (3) ◽  
pp. 483-489 ◽  
Author(s):  
Fu-Ping WEN ◽  
Tan ZHANG ◽  
Zhao-Hui ZHANG ◽  
Ying-Hong PAN
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Proteome Analysis ◽  
Rice Seed

Quantitative trait loci controlling rice seed germination under salt stress

Euphytica ◽  
2010 ◽  
Vol 178 (3) ◽  
pp. 297-307 ◽  
Author(s):  
Zhoufei Wang ◽  
Jianfei Wang ◽  
Yongmei Bao ◽  
Yunyu Wu ◽  
Hongsheng Zhang
Keyword(s):  
Salt Stress ◽  
Quantitative Trait Loci ◽  
Seed Germination ◽  
Quantitative Trait ◽  
Rice Seed ◽  
Trait Loci

Inheritance of Rice Seed Germination Ability under Salt Stress

Rice Science ◽  
2010 ◽  
Vol 17 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Zhou-fei WANG ◽  
Jian-fei WANG ◽  
Yong-mei BAO ◽  
Yun-yu WU ◽  
Xuan SU ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Rice Seed ◽  
Germination Ability

scholarly journals Exogenous melatonin improves seed germination in Limonium bicolor under salt stress

2019 ◽  
Vol 14 (11) ◽  
pp. 1659705 ◽  
Author(s):  
Junpeng Li ◽  
Chen Zhao ◽  
Mingjing Zhang ◽  
Fang Yuan ◽  
Min Chen
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Exogenous Melatonin ◽  
Limonium Bicolor

scholarly journals Identification and fine mapping of qGR6.2, a novel locus controlling rice seed germination under salt stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peng Zeng ◽  
Peiwen Zhu ◽  
Luofeng Qian ◽  
Xumei Qian ◽  
Yuxin Mi ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Candidate Genes ◽  
Germination Rate ◽  
Tyrosine Phosphatase ◽  
Rice Seed ◽  
Microarray Database ◽  
Family Protein ◽  
Germination Traits ◽  
Causal Candidate

Abstract Background Rice growth is frequently affected by salinity. When exposed to high salinity, rice seed germination and seedling establishment are significantly inhibited. With the promotion of direct-seeding in Asia, improving rice seed germination under salt stress is crucial for breeding. Results In this study, an indica landrace Wujiaozhan (WJZ) was identified with high germinability under salt stress. A BC1F2 population derived from the crossing WJZ/Nip (japonica, Nipponbare)//Nip, was used to quantitative trait loci (QTL) mapping for the seed germination rate (GR) and germination index (GI) under H2O and 300 mM NaCl conditions. A total of 13 QTLs were identified, i.e. ten QTLs under H2O conditions and nine QTLs under salt conditions. Six QTLs, qGR6.1, qGR8.1, qGR8.2, qGR10.1, qGR10.2 and qGI10.1 were simultaneously identified under two conditions. Under salt conditions, three QTLs, qGR6.2, qGR10.1 and qGR10.2 for GR were identified at different time points during seed germination, which shared the same chromosomal region with qGI6.2, qGI10.1 and qGI10.2 for GI respectively. The qGR6.2 accounted for more than 20% of phenotypic variation under salt stress, as the major effective QTL. Furthermore, qGR6.2 was verified via the BC2F2 population and narrowed to a 65.9-kb region with eleven candidate genes predicted. Based on the microarray database, five candidate genes were found with high transcript abundances at the seed germination stage, of which LOC_Os06g10650 and LOC_Os06g10710 were differentially expressed after seed imbibition. RT-qPCR results showed the expression of LOC_Os06g10650 was significantly up-regulated in two parents with higher levels in WJZ than Nip during seed germination under salt conditions. Taken together, it suggests that LOC_Os06g10650, encoding tyrosine phosphatase family protein, might be the causal candidate gene for qGR6.2. Conclusions In this study, we identified 13 QTLs from a landrace WJZ that confer seed germination traits under H2O and salt conditions. A major salt-tolerance-specific QTL qGR6.2 was fine mapped to a 65.9-kb region. Our results provide information on the genetic basis of improving rice seed germination under salt stress by marker-assisted selection (MAS).

scholarly journals Melatonin Promotes Seed Germination Under Salt Stress by Regulating ABA and GA3 in Cotton (Gossypium Hirsutum L.)

2020 ◽  
Author(s):  
Li Chen ◽  
Bin Lu ◽  
Liantao Liu ◽  
Wenjing Duan ◽  
Dan Jiang ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Seed Germination ◽  
Plant Hormone ◽  
Exogenous Melatonin ◽  
Cotton Seeds ◽  
Aba Content ◽  
Ga Content ◽  
Endogenous Melatonin

Abstract Background: Although previous studies have found that melatonin can promote seed germination, the phytohormone regulation mechanism by which exogenous melatonin mediates salt tolerance during cotton seed germination is still largely unknown. We investigated the effect of melatonin on the germination traits and physiological parameters of GXM9 cotton seeds (Gossypium hirsutum L.) under three salt stress treatments (CK, germination of seeds pretreated with water alone; S, germination of seeds pretreated in 150 mM NaCl under salt stress; SM, germination of seeds pretreated in 20 µM melatonin under 150 mM NaCl solution) in the laboratory.Results: We found that salt stress (150 mM) inhibited cotton seed germination and endogenous melatonin accumulation, and pretreatment with 20 µM exogenous melatonin enhanced the cotton germination rate and hypocotyl length as well as the content of endogenous melatonin during seed germination. This suggests that exogenous melatonin promotes seed germination from a morphological perspective. The contents of starch, α-amylase (EC3.3.1.1), β-galactosidase (EC3.2.1.23), abscisic acid (ABA), and gibberellin (GA) were determined simultaneously. The results showed that the α-amylase and β-galactosidase contents in the cotton seeds decreased by 56.97% and 20.18%, respectively, under salt stress compared with the control, while the starch content increased by 11.53% compared with the control at day 7. The ABA content increased by 25.18% and GA content decreased by 27.99% under salt stress compared with the control at 24 h. When exogenous melatonin was applied to the cotton seeds, the content of α-amylase and β-galactosidase increased by 121.77% and 32.76%, respectively, whereas the starch contents decreased by 13.55% compared with the S treatment at day 7. Similarly, the ABA content increased by 12.20% and the GA content increased by 4.77% at 24 h. To elucidate the molecular mechanism by which melatonin promotes seed germination under salt stress, the effects of ABA- and GA-related genes on plant hormone signal transduction were analyzed by quantitative real-time PCR and RNA sequencing. The results indicated that melatonin regulated the expression of ABA and GA genes in the plant signal transduction pathway, induced embryo root development and seed germination, and alleviated dormancy. We found that the expression of the ABA signaling gene GhABF2 was up-regulated and GhDPBF2 was down-regulated, and the expression of GA signaling genes (e.g., GhGID1C and GhGID1B) was up-regulated by melatonin.Conclusions: We discovered that melatonin enhances salt tolerance in cotton seeds by regulating ABA and GA and by mediating the expression of hormone-related genes in plant hormone signal transduction. This should help us to explore the regulatory mechanisms of cotton resistance and provide a foundation for the cultivation of new varieties.

scholarly journals Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0228241 ◽  
Author(s):  
Li Chen ◽  
Liantao Liu ◽  
Bin Lu ◽  
Tongtong Ma ◽  
Dan Jiang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Gossypium Hirsutum ◽  
Osmotic Regulation ◽  
Gossypium Hirsutum L ◽  
Exogenous Melatonin
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