Preservation methods of Tolypothrix tenuis for use as a cyanobacterial fertilizer

Tropical black bolete, Phlebopus portentosus, provides various nutritional benefits and natural antioxidants to humans. In this study, the chemical composition, phenolic compounds, and antioxidant and antimicrobial activities of fresh mushroom samples and samples stored for a period of one year using different preservation methods (drying, brining, and frozen) were investigated. The results indicated that the brining method significantly reduced the protein and fat contents of the mushrooms. The polyphenol and flavonoid contents of the frozen sample were not significantly different from that of the fresh sample. The results revealed that an inhibition value of 50% (IC50) for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay of the extract of the dried and frozen samples was not statistically different from that of the fresh sample. The IC50 value of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and ferric reducing antioxidant power (FRAP) value in the extract of the frozen sample were not found to be significantly different from those of the fresh sample. Furthermore, the lowest degree of antioxidant activity was found in the extract of the brined sample. Additionally, the antimicrobial activities of the extracts of the fresh and frozen samples were not significantly different and both extracts could have inhibited the growth of all tested Gram-positive bacteria and Pseudomonas aeruginosa.

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Effectiveness of combined preservation methods to extend the shelf life of Morcilla de Burgos

Meat Science ◽

10.1016/j.meatsci.2008.07.015 ◽

2009 ◽

Vol 81 (1) ◽

pp. 171-177 ◽

Cited By ~ 32

Author(s):

Ana M. Diez ◽

Eva M. Santos ◽

Isabel Jaime ◽

Jordi Rovira

Keyword(s):

Shelf Life ◽

Preservation Methods

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Effects of Preservation Methods in the Composition of the Placental and Reflected Regions of the Human Amniotic Membrane

Cells Tissues Organs ◽

10.1159/000515448 ◽

2021 ◽

pp. 1-11

Author(s):

Jéssica Tereza Guedes de Oliveira Moraes ◽

Maíra Maftoum Costa ◽

Paula Cristina Santos Alves ◽

Luciana Barros Sant’Anna

Keyword(s):

Regenerative Medicine ◽

Amniotic Membrane ◽

Structural Integrity ◽

Storage Period ◽

Soluble Proteins ◽

Control Group ◽

Human Amniotic Membrane ◽

Glycerol Solution ◽

Preservation Methods ◽

Preservation Method

The human amniotic membrane (AM) is emerging as an interesting biomaterial for regenerative medicine due to its biological and mechanical proprieties. The beneficial effects of the AM are probably related to its bioactive factors produced by local cells and stored in the stromal matrix. However, the search for a preservation method capable of preserving AM properties remains a challenge. The aim of this study was to evaluate important features of 2 anatomical regions of the human AM (reflected and placental amnion) after different preservation methods. For this purpose, human placentas were harvested and processed for AM isolation and storage at 2 different conditions: room temperature for 18 h in DMEM (fresh AM) and −80°C in DMEM/glycerol solution for 30 days (cryopreserved AM). After the storage period, the structural integrity of the membrane was assessed by histological and Picrosirius polarization analysis, cellular viability analysis was performed using the MTT assay, and the soluble proteins were quantified with the Qubit Protein Assay Kit. Both preservation protocols reduced the cell viability, mainly in the placental amnion region of the AM, but preserved the morphology of epithelial and stromal layers, as well as the organization and distribution of collagen fibers. There was a reduction in soluble proteins only in fresh AM. Importantly, the cryopreserved AM group presented the same concentration as the control group. In conclusion, the cryopreservation using DMEM/glycerol was ideal for preserving the structural integrity and soluble protein content, indicating the feasibility of this method in preserving AM for its use in regenerative medicine.

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Comparison of different skin preservation methods with gamma irradiation

Burns ◽

10.1016/j.burns.2017.01.003 ◽

2017 ◽

Vol 43 (4) ◽

pp. 804-811 ◽

Cited By ~ 1

Author(s):

Linda Guerrero ◽

Bernardo Camacho

Keyword(s):

Gamma Irradiation ◽

Preservation Methods

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Effect of two preservation methods on the viability and enzyme production of a recombinant Komagataella phaffii (Pichia pastoris) strain

Cryobiology ◽

10.1016/j.cryobiol.2021.12.004 ◽

2021 ◽

Author(s):

Alvarado-Fernández Angela María ◽

Edwin Alexander Rodríguez-López ◽

Angela Johana Espejo-Mojica ◽

Angela Rocío Mosquera-Arévalo ◽

Carlos Javier Alméciga-Díaz ◽

...

Keyword(s):

Pichia Pastoris ◽

Enzyme Production ◽

Pastoris Strain ◽

Preservation Methods ◽

Komagataella Phaffii

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Optimizing Protocols for High-Quality RNA Extraction from Blood and Liver Tissues of the Broad-Snouted Caiman

Russian Journal of Herpetology ◽

10.30906/1026-2296-2021-28-4-197-204 ◽

2021 ◽

Vol 28 (4) ◽

pp. 197-204

Author(s):

Evelyn Cecilia López González ◽

Lucía Magdalena Odetti ◽

Gisela Laura Poletta ◽

Nancy Denslow ◽

Kevin J. Kroll ◽

...

Keyword(s):

Rna Extraction ◽

Biological Processes ◽

High Quality ◽

Efficient Procedure ◽

Physiological Conditions ◽

Environmental Perturbation ◽

Preservation Methods ◽

Cell Tissue ◽

And Storage ◽

Liver Tissues

Transcriptomic information provides fundamental insights into biological processes and can be used to determine gene expression in cell, tissue, or organism under specific physiological conditions, or in response to any environmental perturbation. Extraction of high quality RNA is a challenging step mainly in non-traditional organisms, and protocols for preservation and isolation need to be adjusted in many cases. In the present work, we aimed to develop a protocol for preservation and isolation of high-quality and quantity of RNA from blood and liver tissues of Caiman latirostris. Three preservation methods were tested: 1) flash freezing (LN2) and storage at –80°C; 2) RNAlater® conservation with progressive cooling up to –80°C); 3) preservation in TRIzol® reagent, flash freezing in LN2 and storage at –80°C. Methods 1 and 2 were tested for liver, while 2 and 3 for blood. Our results showed that both preservation methods resulted in excellent outcomes for liver samples. For blood samples however, TRIzol® preservation was an efficient procedure for adequate RNA quality, quantity, and integrity, while conservation in RNAlater® solution was inadequate in both quality and quantity for an optimal RNA extraction. Appropriate protocols were established for each tissue and are being used now for transcriptomic studies in this sentinel organism.

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