Effects of indole-3-acetic acid (IAA), jasmonic acid (JA), and gibberellic acid (GA3) on the direct regeneration of Gelidium floridanum explants

2021 ◽  
Author(s):  
Deonir Batista ◽  
Débora Tomazi Pereira ◽  
Mariana Fontes Carvalho ◽  
Luciane Cristina Ouriques ◽  
Carmen Simioni
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Jasmonic Acid ◽  
Direct Regeneration ◽  
Gelidium Floridanum ◽  
Indole 3 Acetic Acid

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

Dendrobium Hybrid Flower Number and Pedicel Curvature is Influenced by the Application of Gibberellic Acid and Indole-3-acetic Acid

2021 ◽  
Vol 48 (6) ◽  
pp. 740-745
Author(s):  
Muhammad-Asyraf Khairul-Anuar ◽  
Purabi Mazumdar ◽  
Sam Lum ◽  
Jennifer Ann Harikrishna
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Flower Number ◽  
Indole 3 Acetic Acid

scholarly journals Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts

Molecules ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 374 ◽  
Author(s):  
Chang Park ◽  
Hyeon Yeo ◽  
Yun Park ◽  
Abubaker Morgan ◽  
Mariadhas Valan Arasu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Fagopyrum Esculentum ◽  
Common Buckwheat ◽  
Fagopyrum Esculentum Moench ◽  
Indole 3 Acetic Acid

scholarly journals Micropropagation of Mahonia ‘Soft Caress’

HortScience ◽  
2011 ◽  
Vol 46 (7) ◽  
pp. 1010-1014 ◽  
Author(s):  
Todd J. Rounsaville ◽  
Darren H. Touchell ◽  
Thomas G. Ranney ◽  
Frank A. Blazich
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Growth Regulator ◽  
Light Treatment ◽  
Ex Vitro ◽  
Basal Media ◽  
Basal Salts ◽  
Future Work ◽  
Indole 3 Acetic Acid

Mahonia ‘Soft Caress’ is a unique new cultivar exhibiting a compact form and delicate evergreen leaves. Protocols for micropropagation of M. ‘Soft Caress’ were developed to expedite multiplication and serve as a foundation for future work with other taxa of Mahonia Nutt. Combinations of sucrose at 30 or 45 g·L−1 in conjunction with Gamborg B5 (B5), Quoirin and Lepoivre (QL), and Murashige and Skoog (MS) basal media as well as other selected growth regulator treatments were evaluated as multiplication media. Rooting of microcuttings was conducted in vitro using combinations of indole-3-butyric acid (IBA) at 0, 2, 4, 8, or 16 μM under either light or dark. Quick dip treatments with aqueous solutions of the potassium (K) salt (K-salt) of IBA at 0, 5.2, 10.4, 20.7, or 41.4 μM were tested in a second experiment for ex vitro rooting. Media containing B5 basal salts and vitamins supplemented with sucrose at 30 g·L−1, 5 μM 6-benzylaminopurine, 5 μM kinetin, 0.5 μM indole-3-acetic acid, and 2.5 μM gibberellic acid yielded 2.80 ± 0.14 microshoots with a mean length of 14.76 ± 0.63 mm over a 6-week culture period and was an optimal multiplication media. Light treatment and IBA concentration had a significant effect on rooting percentages. Microcuttings treated with 8 μM IBA and maintained in the dark resulted in the best rooting (70%) and ex vitro establishment.

Germination Behavior of Florida Pusley Seeds. II. Effects of Germination-Stimulating Chemicals

Weed Science ◽  
1976 ◽  
Vol 24 (4) ◽  
pp. 349-352 ◽  
Author(s):  
K. B. Paul ◽  
J. L. Crayton ◽  
P. K. Biswas
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Adenosine Monophosphate ◽  
Cold Stratification ◽  
Germination Inhibition ◽  
Light Requirement ◽  
Seed Germinability ◽  
Indole 3 Acetic Acid

Freshly harvested seeds of Florida pusley (Richardia scabraL.) require light for germination. Gibberellic acid (GA3) successfully replaced the light requirement of both scarified and unscarified seeds, while cyclic 3′,5′-adenosine monophosphate (C-AMP) stimulated the dark germination of scarified seeds only. Cold-stratification reduced seed germinability. The germination inhibition by cold-stratification was reversed by both GA3and KNO3treatments. Indole-3-acetic acid (IAA), kinetin, thiourea, and acetylcholine (ACh), either had no effect or reduced the germination percentages of Florida pusley seeds under all conditions studied.

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