Deregulation of imprinted genes expression and epigenetic regulators in placental tissue from intrauterine growth restriction

2021 ◽  
Author(s):  
Carla Caniçais ◽  
Sara Vasconcelos ◽  
Carla Ramalho ◽  
C. Joana Marques ◽  
Sofia Dória
Keyword(s):  
Intrauterine Growth Restriction ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Imprinted Genes ◽  
Intrauterine Growth ◽  
Genes Expression ◽  
Epigenetic Regulators

Differences in gene expression dependent on sampling site in placental tissue of fetuses with intrauterine growth restriction

Placenta ◽  
2010 ◽  
Vol 31 (3) ◽  
pp. 178-185 ◽  
Author(s):  
A.A. Tzschoppe ◽  
E. Struwe ◽  
H.G. Dörr ◽  
T.W. Goecke ◽  
M.W. Beckmann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intrauterine Growth Restriction ◽  
Sampling Site ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Intrauterine Growth

Microarray analysis of placental tissue in intrauterine growth restriction

2010 ◽  
Vol 72 (2) ◽  
pp. 241-247 ◽  
Author(s):  
E. Struwe ◽  
G. Berzl ◽  
R. Schild ◽  
H. Blessing ◽  
L. Drexel ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Intrauterine Growth Restriction ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Intrauterine Growth

scholarly journals The role of apoptosis in the complex pathogenesis of the most common obstetrics and gynaecology diseases

2020 ◽  
Vol 107 (1) ◽  
pp. 106-119
Author(s):  
P. Kovács ◽  
József Gábor Joó ◽  
V. Tamás ◽  
Z. Molnár ◽  
D. Burik-Hajas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intrauterine Growth Restriction ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Tissue Samples ◽  
Intrauterine Growth ◽  
Pcr Method ◽  
Bax Gene ◽  
Gene Expression Levels

AbstractPurposeWe aimed to assess the etiological role of apoptotic genes Bcl-2 and Bax in the background of major obstetric and gynaecological diseases.MethodsPlacental tissue samples were collected from 101 pregnancies with intrauterine growth restriction and 104 pregnancies with premature birth with 140 controll samples from term, eutrophic newborns. In addition, gene expression assessment of the genes Bax and Bcl-2 was performed in 101 uterine leiomyoma tissue samples at our disposal with 110 control cases. Gene expression levels were assessed by PCR method.ResultsThe expression of the Bcl-2 gene was decreased in placental samples with intrauterine growth restriction. Significant overexpression of the proapoptotic Bax gene was detected in samples from premature infants. Antiapoptotic Bcl-2 gene expression was found to be significantly increased in fibroid tissues.ConclusionApoptosis plays a crucial role in the development of the most common OB/GYN conditions. Decrease in the placental expression of the antiapoptotic gene Bcl-2 may upset the balance of programmed cell death.

Unbalanced Placental Expression of Imprinted Genes in Human Intrauterine Growth Restriction

Placenta ◽  
2006 ◽  
Vol 27 (6-7) ◽  
pp. 540-549 ◽  
Author(s):  
J. McMinn ◽  
M. Wei ◽  
N. Schupf ◽  
J. Cusmai ◽  
E.B. Johnson ◽  
...  
Keyword(s):  
Intrauterine Growth Restriction ◽  
Growth Restriction ◽  
Imprinted Genes ◽  
Intrauterine Growth

Placental mitochondrial content and function in intrauterine growth restriction and preeclampsia

2014 ◽  
Vol 306 (4) ◽  
pp. E404-E413 ◽  
Author(s):  
C. Mandò ◽  
C. De Palma ◽  
T. Stampalija ◽  
G. M. Anelli ◽  
M. Figus ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Intrauterine Growth Restriction ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Elective Cesarean Section ◽  
Mrna Levels ◽  
Respiratory Chain Complexes ◽  
Mitochondrial Content ◽  
Intrauterine Growth ◽  
Cytotrophoblast Cells

Intrauterine growth restriction (IUGR) and pregnancy hypertensive disorders such as preeclampsia (PE) associated with IUGR share a common placental phenotype called “placental insufficiency”, originating in early gestation when high availability of energy is required. Here, we assess mitochondrial content and the expression and activity of respiratory chain complexes (RCC) in placental cells of these pathologies. We measured mitochondrial (mt)DNA and nuclear respiratory factor 1 ( NRF1) expression in placental tissue and cytotrophoblast cells, gene and protein expressions of RCC (real-time PCR and Western blotting) and their oxygen consumption, using the innovative technique of high-resolution respirometry. We analyzed eight IUGR, six PE, and eight uncomplicated human pregnancies delivered by elective cesarean section. We found lower mRNA levels of complex II, III, and IV in IUGR cytotrophoblast cells but no differences at the protein level, suggesting a posttranscriptional compensatory regulation. mtDNA was increased in IUGR placentas. Both mtDNA and NRF1 expression were instead significantly lower in their isolated cytotrophoblast cells. Finally, cytotrophoblast RCC activity was significantly increased in placentas of IUGR fetuses. No significant differences were found in PE placentas. This study provides genuine new data into the complex physiology of placental oxygenation in IUGR fetuses. The higher mitochondrial content in IUGR placental tissue is reversed in cytotrophoblast cells, which instead present higher mitochondrial functionality. This suggests different mitochondrial content and activity depending on the placental cell lineage. Increased placental oxygen consumption might represent a limiting step in fetal growth restriction, preventing adequate oxygen delivery to the fetus.

scholarly journals Alteration of placental haemostatic mechanisms in idiopathic intrauterine growth restriction

2012 ◽  
Vol 17 (2) ◽  
pp. 179 ◽  
Author(s):  
Reggie García-Robles ◽  
Paola Andrea Ayala-Ramírez ◽  
Alejandra Espinosa ◽  
Mercedes Olaya ◽  
Juan Diego Rojas ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Intrauterine Growth Restriction ◽  
Placental Tissue ◽  
Growth Restriction ◽  
Abnormal Development ◽  
Histological Evaluation ◽  
Mrna Levels ◽  
Perinatal Morbidity ◽  
Intrauterine Growth ◽  
Coagulation Abnormalities

Intrauterine growth restriction is a complication of pregnancy with a high probability of perinatal morbidity and mortality. It appears to be caused by abnormal development of placental vasculature. Haemostatic processes are important for the development of the placenta, and an imbalance between procoagulant and anticoagulant factors has been associated with risk of intrauterine growth restriction.<strong> Objective</strong>. To evaluate coagulation abnormalities in placenta of pregnancies complicated with idiopathic intrauterine growth restriction. <strong>Materials and methods</strong>. Five placentas from pregnancies with idiopathic intrauterine growth restriction were compared to 19 controls. We performed gross and histological examination of the placenta. Analysis was made of both mRNA expression by real-time PCR<br />and protein by ELISA of tissue factor and thrombomodulin in placental tissue. <strong>Results</strong>. Results based on histological evaluation were consistent with an increased prothrombotic state in placentas from pregnancies with idiopathic intrauterine growth restriction, and thrombosis of chorionic vessels was the most important finding. The study showed an increased expression of tissue factor protein (p=0.0411) and an increase in the ratio of tissue factor/thrombomodulin mRNA (p=0.0411) and protein (p=0.0215) in placentas from pregnancies with idiopathic intrauterine growth restriction. There were no statistically significant differences neither between cases and controls in the mRNA levels of tissue factor or thrombomodulin nor at the protein level of thrombomodulin. <strong>Conclusion</strong>. Evidence of alteration of local haemostatic mechanisms at the level of the placenta, including abnormal expression of tissue factor and tissue factor/thrombomodulin ratio, in pregnancies that occur with idiopathic intrauterine growth restriction is presented.<br /><strong>Key words</strong>: intrautherine growth restriction, tissue factor, thrombomodulin, placenta diseases.

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