Evaluation of in situ albumin binding surfaces: a study of protein adsorption and platelet adhesion

2010 ◽  
Vol 22 (1) ◽  
pp. 137-149 ◽  
Author(s):  
Sanjukta Guha Thakurta ◽  
Anuradha Subramanian
Keyword(s):  
Protein Adsorption ◽  
Platelet Adhesion ◽  
Albumin Binding

Evaluation of the real-time protein adsorption kinetics on albumin-binding surfaces by dynamic in situ spectroscopic ellipsometry

2012 ◽  
Vol 520 (6) ◽  
pp. 2200-2207 ◽  
Author(s):  
Sanjukta Guha Thakurta ◽  
Hendrik J. Viljoen ◽  
Anuradha Subramanian
Keyword(s):  
Protein Adsorption ◽  
Real Time ◽  
Adsorption Kinetics ◽  
Spectroscopic Ellipsometry ◽  
Albumin Binding ◽  
The Real

Antifouling property of monothiol-terminated bottle-brush poly(methylacrylic acid)-graft-poly(2-methyl-2-oxazoline) copolymer on gold surfaces

2015 ◽  
Vol 3 (9) ◽  
pp. 1921-1930 ◽  
Author(s):  
Xiajun Zheng ◽  
Chong Zhang ◽  
Longchao Bai ◽  
Songtao Liu ◽  
Lin Tan ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Platelet Adhesion ◽  
Gold Surfaces ◽  
Antifouling Property ◽  
Bottle Brush

A series of well-controlled bottle-brush poly(methylacrylic acid)-graft-poly(2-methyl-2-oxazoline) copolymers were grafted to gold surfaces through an in situ aminolysis reaction to reduce protein adsorption and platelet adhesion.

Adherence of platelets to in situ albumin-binding surfaces under flow conditions: role of surface-adsorbed albumin

2012 ◽  
Vol 7 (4) ◽  
pp. 045007 ◽  
Author(s):  
Sanjukta Guha Thakurta ◽  
Robert Miller ◽  
Anuradha Subramanian
Keyword(s):  
Albumin Binding ◽  
Flow Conditions

scholarly journals In Silico Hemostasis Modeling and Prediction

2020 ◽  
Vol 40 (04) ◽  
pp. 524-535
Author(s):  
Dmitry Y. Nechipurenko ◽  
Aleksey M. Shibeko ◽  
Anastasia N. Sveshnikova ◽  
Mikhail A. Panteleev
Keyword(s):  
In Silico ◽  
Physiological Response ◽  
Platelet Adhesion ◽  
State Of The Art ◽  
Thrombus Formation ◽  
The State ◽  
Modeling And Prediction ◽  
Hemostatic System ◽  
Single Enzyme

AbstractComputational physiology, i.e., reproduction of physiological (and, by extension, pathophysiological) processes in silico, could be considered one of the major goals in computational biology. One might use computers to simulate molecular interactions, enzyme kinetics, gene expression, or whole networks of biochemical reactions, but it is (patho)physiological meaning that is usually the meaningful goal of the research even when a single enzyme is its subject. Although exponential rise in the use of computational and mathematical models in the field of hemostasis and thrombosis began in the 1980s (first for blood coagulation, then for platelet adhesion, and finally for platelet signal transduction), the majority of their successful applications are still focused on simulating the elements of the hemostatic system rather than the total (patho)physiological response in situ. Here we discuss the state of the art, the state of the progress toward the efficient “virtual thrombus formation,” and what one can already get from the existing models.

scholarly journals Plasmon-enhanced two-channel in situ Kretschmann ellipsometry of protein adsorption, cellular adhesion and polyelectrolyte deposition on titania nanostructures

2016 ◽  
Vol 24 (5) ◽  
pp. 4812 ◽  
Author(s):  
Judit Nador ◽  
Benjamin Kalas ◽  
Andras Saftics ◽  
Emil Agocs ◽  
Peter Kozma ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Cellular Adhesion ◽  
Titania Nanostructures

An in situ study of protein adsorption on combinatorial Cu–Al films using spectroscopic ellipsometry

2010 ◽  
Vol 81 (1) ◽  
pp. 58-66 ◽  
Author(s):  
M.A. McArthur ◽  
T.M. Byrne ◽  
R.J. Sanderson ◽  
G.P. Rockwell ◽  
L.B. Lohstreter ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Spectroscopic Ellipsometry ◽  
In Situ Study

Honokiol Prodrug Nanoparticles Based on In Situ Albumin Binding for Long Circulation and High Tumor Uptake

2021 ◽  
Vol 12 (10) ◽  
pp. 1589-1595
Author(s):  
Lixue Chen ◽  
Shengnan Li ◽  
Yanfang Ding ◽  
Changyuan Wang ◽  
Sitong Zhang ◽  
...  
Keyword(s):  
Albumin Binding ◽  
Tumor Uptake ◽  
High Tumor Uptake

scholarly journals Cathepsin B-Overexpressed Tumor Cell Activatable Albumin-Binding Doxorubicin Prodrug for Cancer-Targeted Therapy

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 83
Author(s):  
Hanhee Cho ◽  
Man Kyu Shim ◽  
Suah Yang ◽  
Sukyung Song ◽  
Yujeong Moon ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cathepsin B ◽  
Half Life ◽  
Albumin Binding ◽  
Normal Tissues ◽  
Cleavable Peptide ◽  
Tumor Accumulation ◽  
Maleimide Group ◽  
Cancer Targeted Therapy

Prodrugs are bioreversible medications that should undergo an enzymatic or chemical transformation in the tumor microenvironment to release active drugs, which improve cancer selectivity to reduce toxicities of anticancer drugs. However, such approaches have been challenged by poor therapeutic efficacy attributed to a short half-life and low tumor targeting. Herein, we propose cathepsin B-overexpressed tumor cell activatable albumin-binding doxorubicin prodrug, Al-ProD, that consists of a albumin-binding maleimide group, cathepsin B-cleavable peptide (FRRG), and doxorubicin. The Al-ProD binds to in situ albumin, and albumin-bound Al-ProD indicates high tumor accumulation with prolonged half-life, and selctively releases doxorubicin in cathepsin B-overexpressed tumor cells, inducing a potent antitumor efficacy. Concurrently, toxicity of Al-ProD toward normal tissues with innately low cathepsin B expression is significantly reduced by maintaining an inactive state, thereby increasing the safety of chemotherapy. This study offers a promising approach for effective and safe chemotherapy, which may open new avenues for drug design and translational medicine.

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