Adherence of platelets to in situ albumin-binding surfaces under flow conditions: role of surface-adsorbed albumin

2012 ◽  
Vol 7 (4) ◽  
pp. 045007 ◽  
Author(s):  
Sanjukta Guha Thakurta ◽  
Robert Miller ◽  
Anuradha Subramanian
Keyword(s):  
Albumin Binding ◽  
Flow Conditions

scholarly journals Liquid Phase Furfural Oxidation under Uncontrolled pH in Batch and Flow Conditions: The Role of In Situ Formed Base

Catalysts ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 73 ◽  
Author(s):  
Alessandra Roselli ◽  
Yuri Carvalho ◽  
Franck Dumeignil ◽  
Fabrizio Cavani ◽  
Sébastien Paul ◽  
...  
Keyword(s):  
Reaction Medium ◽  
Pure Oxygen ◽  
Continuous Stirred Tank Reactor ◽  
Flow Conditions ◽  
Furoic Acid ◽  
Continuous Stirred Tank ◽  
Supported Gold Nanoparticles ◽  
Supported Gold

Selective oxidation of furfural to furoic acid was performed with pure oxygen in aqueous phase under mild conditions and uncontrolled pH using hydrotalcite-supported gold nanoparticles as catalyst. Hydrotalcites with different Mg: Al ratios were tested as support. The effects of reaction time, temperature and furfural/catalyst ratio were evaluated. The catalyst Au/HT 4:1 showed the highest activity and selectivity to the desired product, achieving a complete conversion of furfural to furoic acid after 2 h at 110 °C. Further, stability tests were carried out in a continuous stirred-tank reactor and a progressive deactivation of the catalyst due to the leaching of Mg2+ cations from the support inducing changes in the pH of the reaction medium was observed.

Platelet and Shear Rate Promote Tumor Cell Adhesion to Human Endothelial Extracellular Matrix - Absence of a Role for Platelet Cyclooxygenase

1989 ◽  
Vol 61 (03) ◽  
pp. 485-489 ◽  
Author(s):  
Eva Bastida ◽  
Lourdes Almirall ◽  
Antonio Ordinas
Keyword(s):  
Cell Adhesion ◽  
Shear Rate ◽  
Tumor Cell ◽  
Umbilical Vein ◽  
Blood Platelets ◽  
Flow Conditions ◽  
Tumor Cell Adhesion ◽  
Rate Dependent ◽  
Static Conditions

SummaryBlood platelets are thought to be involved in certain aspects of malignant dissemination. To study the role of platelets in tumor cell adherence to vascular endothelium we performed studies under static and flow conditions, measuring tumor cell adhesion in the absence or presence of platelets. We used highly metastatic human adenocarcinoma cells of the lung, cultured human umbilical vein endothelial cells (ECs) and extracellular matrices (ECM) prepared from confluent EC monolayers. Our results indicated that under static conditions platelets do not significantly increase tumor cell adhesion to either intact ECs or to exposed ECM. Conversely, the studies performed under flow conditions using the flat chamber perfusion system indicated that the presence of 2 × 105 pl/μl in the perfusate significantly increased the number of tumor cells adhered to ECM, and that this effect was shear rate dependent. The maximal values of tumor cell adhesion were obtained, in presence of platelets, at a shear rate of 1,300 sec-1. Furthermore, our results with ASA-treated platelets suggest that the role of platelets in enhancing tumor cell adhesion to ECM is independent of the activation of the platelet cyclooxygenase pathway.

{10-12} Twinning Mechanism During In Situ Micro-Tensile Loading of Pure Mg: Role of Basal Slip and Twin-Twin Boundary Interaction

2020 ◽  
Author(s):  
Nicolò Maria della Ventura ◽  
Szilvia Kalácska ◽  
Daniele Casari ◽  
Thomas Edward James Edwards ◽  
Johann Michler ◽  
...  
Keyword(s):  
Twin Boundary ◽  
Basal Slip ◽  
Tensile Loading ◽  
Boundary Interaction

The role of (bio)surfactant sorption in promoting the bioavailability of nutrients localized at the solid-water interface

1999 ◽  
Vol 39 (7) ◽  
pp. 91-98 ◽  
Author(s):  
Ryan N. Jordan ◽  
Eric P. Nichols ◽  
Alfred B. Cunningham
Keyword(s):  
Mass Transfer ◽  
Cell Membrane ◽  
Substrate Concentration ◽  
Water Interface ◽  
Industrial Systems ◽  
Solid Liquid Interface ◽  
Solid Liquid ◽  
Surfactant Sorption

Bioavailability is herein defined as the accessibility of a substrate by a microorganism. Further, bioavailability is governed by (1) the substrate concentration that the cell membrane “sees,” (i.e., the “directly bioavailable” pool) as well as (2) the rate of mass transfer from potentially bioavailable (e.g., nonaqueous) phases to the directly bioavailable (e.g., aqueous) phase. Mechanisms by which sorbed (bio)surfactants influence these two processes are discussed. We propose the hypothesis that the sorption of (bio)surfactants at the solid-liquid interface is partially responsible for the increased bioavailability of surface-bound nutrients, and offer this as a basis for suggesting the development of engineered in-situ bioremediation technologies that take advantage of low (bio)surfactant concentrations. In addition, other industrial systems where bioavailability phenomena should be considered are addressed.

THE ROLE OF BACTERIAL COMMUNITIES ON IN-SITU ARSENIC AND IRON CYCLING IN RIVERBANKS ALONG THE MEGHNA RIVER, BANGLADESH

2020 ◽  
Author(s):  
Kimberly D. Myers ◽  
◽  
Katrina Lee Jewell ◽  
P.S.K. Knappett ◽  
Mehtaz M. Lipsi ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Iron Cycling

scholarly journals Forensic Investigation of Four Monitored Green Infrastructure Inlets

Water ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 1787
Author(s):  
Leena J. Shevade ◽  
Franco A. Montalto
Keyword(s):  
Measurement Uncertainty ◽  
Green Infrastructure ◽  
Stormwater Management ◽  
Catchment Area ◽  
Low Flow ◽  
Inflow Rate ◽  
Forensic Investigation ◽  
Flow Conditions ◽  
The Mean

Green infrastructure (GI) is viewed as a sustainable approach to stormwater management that is being rapidly implemented, outpacing the ability of researchers to compare the effectiveness of alternate design configurations. This paper investigated inflow data collected at four GI inlets. The performance of these four GI inlets, all of which were engineered with the same inlet lengths and shapes, was evaluated through field monitoring. A forensic interpretation of the observed inlet performance was conducted using conclusions regarding the role of inlet clogging and inflow rate as described in the previously published work. The mean inlet efficiency (meanPE), which represents the percentage of tributary area runoff that enters the inlet was 65% for the Nashville inlet, while at Happyland the NW inlet averaged 30%, the SW inlet 25%, and the SE inlet 10%, considering all recorded events during the monitoring periods. The analysis suggests that inlet clogging was the main reason for lower inlet efficiency at the SW and NW inlets, while for the SE inlet, performance was compromised by a reverse cross slope of the street. Spatial variability of rainfall, measurement uncertainty, uncertain tributary catchment area, and inlet depression characteristics are also correlated with inlet PE. The research suggests that placement of monitoring sensors should consider low flow conditions and a strategy to measure them. Additional research on the role of various maintenance protocols in inlet hydraulics is recommended.

How Artefacts Do Leadership: A Ventriloquial Analysis

2021 ◽  
pp. 089331892199807
Author(s):  
Jonathan Clifton ◽  
Fernando Fachin ◽  
François Cooren
Keyword(s):  
Organizational Communication ◽  
Recent Work ◽  
Distributed Leadership ◽  
Network Theory ◽  
Actor Network Theory ◽  
Fine Grained ◽  
Naturally Occurring ◽  
Human Actors

To date there has been little work that uses fine-grained interactional analyses of the in situ doing of leadership to make visible the role of non-human as well as human actants in this process. Using transcripts of naturally-occurring interaction as data, this study seeks to show how leadership is co-achieved by artefacts as an in-situ accomplishment. To do this we situate this study within recent work on distributed leadership and argue that it is not only distributed across human actors, but also across networks that include both human and non-human actors. Taking a discursive approach to leadership, we draw on Actor Network Theory and adopt a ventriloquial approach to sociomateriality as inspired by the Montreal School of organizational communication. Findings indicate that artefacts “do” leadership when a hybrid presence is made relevant to the interaction and when this presence provides authoritative grounds for influencing others to achieve the group’s goals.

scholarly journals Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation

2021 ◽  
Vol 22 (7) ◽  
pp. 3787
Author(s):  
Hussam Ibrahim ◽  
Philipp Reus ◽  
Anna Katharina Mundorf ◽  
Anna-Lena Grothoff ◽  
Valerie Rudenko ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Transcriptional Repression ◽  
Small Gtpase ◽  
Main Role ◽  
Interacting Proteins ◽  
Casein Kinase 1 ◽  
Bona Fide ◽  
Kinetics Of

Repressor protein period (PER) complexes play a central role in the molecular oscillator mechanism of the mammalian circadian clock. While the main role of nuclear PER complexes is transcriptional repression, much less is known about the functions of cytoplasmic PER complexes. We found with a biochemical screen for PER2-interacting proteins that the small GTPase regulator GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1), which has been identified previously as a component of cytoplasmic PER complexes in mice, is also a bona fide component of human PER complexes. We show that in situ GAPVD1 is closely associated with casein kinase 1 delta (CSNK1D), a kinase that regulates PER2 levels through a phosphoswitch mechanism, and that CSNK1D regulates the phosphorylation of GAPVD1. Moreover, phosphorylation determines the kinetics of GAPVD1 degradation and is controlled by PER2 and a C-terminal autoinhibitory domain in CSNK1D, indicating that the regulation of GAPVD1 phosphorylation is a novel function of cytoplasmic PER complexes and might be part of the oscillator mechanism or an output function of the circadian clock.

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