In vitro cytotoxicity of different dental resin-cements on human cell lines

AbstractAdhesive resin-cements are increasingly used in modern dentistry. Nevertheless, released substances from resin materials have been shown to cause cellular toxic effects. Disc-shaped specimens from 12 different resin cements and one conventional zinc phosphate cement were prepared and used for direct stimulation of five different human cell lines via transwell cell culture system or in an indirect way using conditioned cell culture media. Cytotoxicity was determined using LDH and BCA assays. All tested cements led to a decrease of cell viability but to a distinct extent depending on cell type, luting material, and cytotoxicity assay. In general, cements exhibited a more pronounced cytotoxicity in direct stimulation experiments compared to stimulations using conditioned media. Interestingly, the conventional zinc phosphate cement showed the lowest impact on cell viability. On cellular level, highest cytotoxic effects were detected in osteoblastic cell lines. All resin cements reduced cell viability of human cells with significant differences depending on cell type and cement material. Especially, osteoblastic cells demonstrated a tremendous increase of cytotoxicity after cement exposure. Although the results of this in vitro study cannot be transferred directly to a clinical setting, it shows that eluted substances from resin cements may disturb osteoblastic homeostasis that in turn could lead to conditions favoring peri-implant bone destruction. Thus, the wide use of resin cements in every clinical situation should be scrutinized. A correct use with complete removal of all cement residues and a sufficient polymerization should be given the utmost attention in clinical usage.

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CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i11.21924 ◽

2017 ◽

Vol 9 (10) ◽

pp. 240

Author(s):

Durgaiah Gandamalla ◽

Harikiran Lingabathula ◽

Narsimha Reddy Yellu

Keyword(s):

Zinc Oxide ◽

Cell Viability ◽

Human Skin ◽

Cell Lines ◽

Human Cell ◽

Cell Damage ◽

Human Cell Lines ◽

Hep G2 ◽

20 Nm

Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colon (Caco-2) cell cultures in relevance to human risk assessmentMethods: The different concentrations of test TNP 20 and ZNP 20 1-300 µg/ml were exposed to determine the cell viability reduction on four human cell lines after 48 h post exposure using 3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mitochondrial membrane activities of the viable cells were determined with intensity of formazon formation by interpreting ELISA absorbance values at 470 nm.Results: The percent of cytotoxicity was determined by comparing percentage of cell viability reduction of test with that of control. The ZNP 20 produced higher cytotoxicity at the doses 100 (p<0.05) and 300 (p<0.001) µg/ml significantly on tested four human skin (HaCaT), lung (A549), liver (Hep G2) and colon (Caco-2) cells compared to TNP 20. The tested NP induced lesser cytotoxicity at lower concentrations with 1 and 3µg/ml in all the tested four cell lines. The induced cytotoxicity was an indicator for increased intracellular reactive oxygen species which further cause’s major cell damage and cell death.Conclusion: The tested NP were induced greater cytotoxicity in the colon, Liver, lung and skin cells at higher concentrations 100 and 300 µg/ml significantly. The cytotoxicity order of TNP 20 and ZNP 20 at the highest dose (300µg/ml) were concluded as Caco-2>Hep G2>A549>HaCaT for 48 h post exposed cells.

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New inhibitors of sepiapterin reductase. Lack of an effect of intracellular tetrahydrobiopterin depletion upon in vitro proliferation of two human cell lines.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)42807-4 ◽

1992 ◽

Vol 267 (8) ◽

pp. 5599-5607

Author(s):

G.K. Smith ◽

D.S. Duch ◽

M.P. Edelstein ◽

E.C. Bigham

Keyword(s):

Cell Lines ◽

Human Cell ◽

Human Cell Lines ◽

In Vitro Proliferation ◽

Sepiapterin Reductase

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Dicalcin suppresses in vitro trophoblast attachment in human cell lines

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2021.07.030 ◽

2021 ◽

Vol 570 ◽

pp. 206-213

Author(s):

Ryohei Saito ◽

Hiromasa Satoh ◽

Kayo Aoba ◽

Hajime Hirasawa ◽

Naofumi Miwa

Keyword(s):

Cell Lines ◽

Human Cell ◽

Human Cell Lines

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Antifungal Activity of Flocculosin, a Novel Glycolipid Isolated from Pseudozyma flocculosa

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.4.1597-1599.2005 ◽

2005 ◽

Vol 49 (4) ◽

pp. 1597-1599 ◽

Cited By ~ 61

Author(s):

Benjamin Mimee ◽

Caroline Labbé ◽

René Pelletier ◽

Richard R. Bélanger

Keyword(s):

Antifungal Activity ◽

Amphotericin B ◽

Cell Lines ◽

Human Cell ◽

Synergistic Activity ◽

Human Cell Lines ◽

Antifungal Properties ◽

Pseudozyma Flocculosa ◽

In Vitro Antifungal Activity

ABSTRACT Flocculosin, a glycolipid isolated from the yeast-like fungus Pseudozyma flocculosa, was investigated for in vitro antifungal activity. The compound displayed antifungal properties against several pathogenic yeasts. Synergistic activity was observed between flocculosin and amphotericin B, and no significant cytotoxicity was demonstrated when tested against human cell lines.

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Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1

Molecular and Cellular Biology ◽

10.1128/mcb.13.10.6036-6043.1993 ◽

1993 ◽

Vol 13 (10) ◽

pp. 6036-6043

Author(s):

T Ogata ◽

D Ayusawa ◽

M Namba ◽

E Takahashi ◽

M Oshimura ◽

...

Keyword(s):

Cell Lines ◽

Human Cell ◽

Human Fibroblasts ◽

Fibroblast Cell ◽

Chromosome 7 ◽

In Vitro Mutagenesis ◽

Human Cell Lines ◽

First Case ◽

Normal Human

Using nontumorigenic immortalized human cell lines KMST-6 (KMST) and SUSM-1 (SUSM), we attempted to identify the chromosome that carries a putative senescence-related gene(s). These cell lines are the only ones that have been established independently from normal human diploid fibroblasts following in vitro mutagenesis. We first examined restriction fragment length polymorphisms on each chromosome of these immortalized cell lines and their parental cell lines and found specific chromosomal alterations common to these cell lines (a loss of heterozygosity in KMST and a deletion in SUSM) on the long arm of chromosome 7. In addition to these, we also found that introduction of chromosome 7 into these cell lines by means of microcell fusion resulted in the cessation of cell division, giving rise to cells resembling cells in senescence. Introduction of other chromosomes, such as chromosomes 1 and 11, on which losses of heterozygosity were also detected in one of the cell lines (KMST), to either KMST or SUSM cells or of chromosome 7 to several tumor-derived cell lines had no effect on their division potential. These results strongly suggest that a gene(s) affecting limited-division potential or senescence of normal human fibroblasts is located on chromosome 7, probably at the long arm of the chromosome, representing the first case in which a specific chromosome reverses the immortal phenotype of otherwise normal human cell lines.

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