scholarly journals The immunogenic reaction and bone defect repair function of ε-poly-L-lysine (EPL)-coated nanoscale PCL/HA scaffold in rabbit calvarial bone defect

2021 ◽  
Vol 32 (6) ◽  
Author(s):  
Bin Tian ◽  
Na Wang ◽  
Qingsong Jiang ◽  
Lijiao Tian ◽  
Lei Hu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Defect ◽  
Macrophage Infiltration ◽  
T Helper ◽  
Calvarial Bone ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Calvarial Bone Defect ◽  
Helper Type

AbstractTissue engineering is a promising strategy for bone tissue defect reconstruction. Immunogenic reaction, which was induced by scaffolds degradation or contaminating microorganism, influence cellular activity, compromise the efficiency of tissue engineering, or eventually lead to the failure of regeneration. Inhibiting excessive immune response through modulating scaffold is critical important to promote tissue regeneration. Our previous study showed that ε-poly-L-lysine (EPL)-coated nanoscale polycaprolactone/hydroxyapatite (EPL/PCL/HA) composite scaffold has enhanced antibacterial and osteogenic properties in vitro. However, the bone defect repair function and immunogenic reaction of EPL/PCL/HA scaffolds in vivo remains unclear. In the present study, three nanoscale scaffolds (EPL/PCL/HA, PCL and PCL/HA) were transplanted into rabbit paraspinal muscle pouches, and T helper type 1 (Th1), T helper type 2 (Th2), T helper type 17 (Th17), and macrophage infiltration were analyzed after 1 week and 2 weeks to detect their immunogenic reaction. Then, the different scaffolds were transplanted into rabbit calvarial bone defect to compare the bone defect repair capacities. The results showed that EPL/PCL/HA composite scaffolds decreased pro-inflammatory Th1, Th17, and type I macrophage infiltration from 1 to 2 weeks, and increased anti-inflammatory Th2 infiltration into the regenerated area at 2 weeks in vivo, when compared to PCL and PCL/HA. In addition, EPL/PCL/HA showed an enhanced bone repair capacity compared to PCL and PCL/HA when transplanted into rabbit calvarial bone defects at both 4 and 8 weeks. Hence, our results suggest that EPL could regulate the immunogenic reaction and promote bone defect repair function of PCL/HA, which is a promising agent for tissue engineering scaffold modulation.

Cryopreserved platelet-rich plasma enhancing calvarial bone defect repair in rabbits: an observation of outcome

2015 ◽  
Vol 36 (4) ◽  
pp. 391
Author(s):  
Jun CHEN ◽  
Yun-fu ZHAO ◽  
Qing-fu ZHANG ◽  
Guo-qin LIU ◽  
Man-jiao LIU ◽  
...  
Keyword(s):  
Bone Defect ◽  
Platelet Rich Plasma ◽  
Calvarial Bone ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Calvarial Bone Defect

scholarly journals miR-196a-5p Promoted the Osteogenic Differentiation and Calvarial Bone Defect Repair of Wharton’s Jelly Umbilical Cord Stem Cells

2021 ◽  
Author(s):  
Yantong Wang ◽  
Simin Zhang ◽  
Haoqing Yang ◽  
Yangyang Cao ◽  
Dianqin Yu ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Defect ◽  
Bone Repair ◽  
Calvarial Bone ◽  
Bone Defect Repair ◽  
Alp Activity ◽  
Defect Repair ◽  
Umbilical Cord Stem Cells ◽  
Calvarial Bone Defect

Abstract Background: To investigate the effect of miR‐196a-5p on the osteogenic differentiation and defected bone repair of Wharton’s jelly umbilical cord stem cells (WJCMSCs). Methods: miR‐196a-5p mimic or inhibitor was applied to overexpress or silence miR‐196a-5p expression in WJCMSCs. The alkaline phosphatase (ALP) activity, mineralization ability, and osteogenic markers expression were used to test WJCMSCs osteogenic potential in vitro. Calvarial bone defect model of rat was used to evaluate WJCMSCs bone regeneration ability in vivo. mRNA microarray was used to reveal the underling mechanisms that miR‐196a-5p regulated bone repair.Results: miR-196a-5p inhibition reduced the ALP activity, mineralization ability, and level of osteogenic markers OCN, DSPP, DMP1 and BSP, while miR-196a-5p overexpression enhanced the ALP activity, mineralization ability, and level of OCN, DSPP, DMP1 and BSP of WJCMSCs in vitro. Next, the micro-CT and histopathology results showed miR-196a-5p-overexpressed-WJCMSCs obviously promoted the new bone tissue regeneration and calvarial bone defect repair after MSCs transplanted for 12 weeks. Further, mRNA microarray of miR-196a-5p-overexpressed-WJCMSCs revealed totally 959 significantly differentially expressed genes (DEGs), among which 34 upregulated and 925 downregulated. Also, 241 miR-196a-5p targeted genes were predicted by using miRNA targeted websites and only 19 predicted genes were consistent with microarray results. On this basis, one significantly downregulated gene SERPINB2 was selected and revealed that SERPINB2 deletion obviously enhanced the ALP activity and mineralization ability of WJCMSCs in vitro.Conclusions: miR-196a-5p promoted the osteogenic differentiation potential and calvarial bone defect repair ability of WJCMSCs. And SERPINB2 acted as one key downstream gene to participate in the miR-196a-5p promoted MSCs osteogenic differentiation.

scholarly journals The mineralization, drug release and in vivo bone defect repair properties of calcium phosphates/PLA modified tantalum scaffolds

RSC Advances ◽  
2020 ◽  
Vol 10 (13) ◽  
pp. 7708-7717 ◽  
Author(s):  
Rong Zhou ◽  
Hai-Jian Ni ◽  
Jin-Hui Peng ◽  
Ning Liu ◽  
Shu Chen ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Drug Release ◽  
Bone Defect ◽  
High Performance ◽  
Calcium Phosphates ◽  
Sustained Drug Release ◽  
Bone Defect Repair ◽  
Defect Repair

Calcium phosphate modified tantalum scaffolds displayed high performance on mineralization, sustained drug release and in vivo bone defect repair.

In vivo model for frontal sinus and calvarial bone defect obliteration with bioactive glass S53P4 and hydroxyapatite

2001 ◽  
Vol 58 (3) ◽  
pp. 261-269 ◽  
Author(s):  
Matti J. Peltola ◽  
Kalle M. J. Aitasalo ◽  
Jouko T. K. Suonp�� ◽  
Antti Yli-Urpo ◽  
Pekka J. Laippala
Keyword(s):  
Bioactive Glass ◽  
Bone Defect ◽  
Frontal Sinus ◽  
In Vivo Model ◽  
Calvarial Bone ◽  
Calvarial Bone Defect

scholarly journals Tissue engineering using mesenchymal stem cells with periosteal wrap for bone defect repair in rabbits

2015 ◽  
Vol 2 (6) ◽  
pp. 340
Author(s):  
Trung-Hau Lê Thua ◽  
Dang-Nhat Pham ◽  
Khanh-Linh Lê ◽  
Minh-Tuan Lê ◽  
Quang-Ton-Quyen Nguyen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Bone Defect Repair ◽  
Defect Repair
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