Green Synthesis of Reduced Graphene oxide Nanosheets Using Leaf Extract of Lantana camara and Its In-Vitro Biological Activities

Author(s):  
Nathiya Thiyagarajulu ◽  
Sivakumar Arumugam
2020 ◽  
Vol 2 (1) ◽  
pp. 93

Graphene oxide nanoparticles has found immense application in bio therapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized reduced graphene oxide (rGO) was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic analysis primarily detected the shift in the absorption peak from 232 to 287 nm confirming the reduction of TP-rGO. FTIR spectra of TP-rGO confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. Raman spectroscopic analysis identified successful reduction of GO through the presence of D band at 1329 cm-1 and G band at 1577 cm-1. XRD spectra of TP-rGO showed diffraction peak 2θ at 25o confirming the presence of reduced GO. SEM analysis revealed the presence of graphene nanosheets appearing as large aggregated structure. The bioreduced rGO showed anti-oxidant effect against DPPH radical observed in concentration dependent manner. The TP-rGO nanosheets exhibited bactericidal effect against gram positive bacteria: B.subitils (18 mm) and S. epidermis (9 mm) and gram negative bacteria: E.coli (14 mm) and P. aeruginosa (6 mm) at 100 µg/ml confirming the enhanced anti-bacterial effects of TP-rGO against gram positive pathogens. Hence, it is highlighted that leaf extract of Tridax procumbens act as green reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.


2020 ◽  
Vol 11 (3) ◽  
pp. 9975-9984

Graphene oxide nanoparticles have found immense application in biotherapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide (rGO) using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized TP-rGO was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic primarily detected the absorption peak from 232 to 287 nm. XRD spectra showed diffraction peak 2θ at 25o, confirming the presence of reduced GO. FTIR and Raman spectra confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. EDAX revealed the presence of carbon (74%) and oxygen (24%). TEM analysis revealed the presence of graphene nanosheets structure appearing as a thin sheet stacked with one another. The bioreduced rGO showed an antioxidant effect against DPPH radical observed in a concentration-dependent manner. The TP-rGO nanosheets were confirming the enhanced antibacterial effects against gram-positive pathogens than gram-negative bacteria. Hence, it is highlighted that leaf extract of T. procumbens act as a green, reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.


RSC Advances ◽  
2015 ◽  
Vol 5 (111) ◽  
pp. 91532-91543 ◽  
Author(s):  
Monireh Atarod ◽  
Mahmoud Nasrollahzadeh ◽  
S. Mohammad Sajadi

Herein, we describe a green and eco-friendly synthesis method for preparing a Cu/RGO/Fe3O4 nanocomposite through biological reduction of graphene oxide and Cu2+, Fe3+ ions using Euphorbia wallichii leaf extract as a reducing and stabilizing agent.


2015 ◽  
Vol 5 (2) ◽  
pp. 1251-1260 ◽  
Author(s):  
Najrul Hussain ◽  
Pranjal Gogoi ◽  
Vedi Kuyil Azhaganand ◽  
Manjusha V. Shelke ◽  
Manash R. Das

The Cu(0) nanoparticle-rGO composites exhibit excellent catalytic activity for the synthesis of symmetrical biaryls from arylboronic acids under microwave irradiation.


2015 ◽  
Vol 5 (2) ◽  
pp. 1334-1334
Author(s):  
Najrul Hussain ◽  
Pranjal Gogoi ◽  
Vedi Kuyil Azhaganand ◽  
Manjusha V. Shelke ◽  
Manash R. Das

Correction for ‘Green synthesis of stable Cu(0) nanoparticles onto reduced graphene oxide nanosheets: a reusable catalyst for the synthesis of symmetrical biaryls from arylboronic acids under base-free conditions’ by Najrul Hussain et al., Catal. Sci. Technol., 2015, DOI: 10.1039/c4cy01229a


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