scholarly journals Green Synthesis of Reduced Graphene Nanosheets using Leaf Extract of Tridax procumbens and its Potential In Vitro Biological Activities

2020 ◽  
Vol 11 (3) ◽  
pp. 9975-9984
Keyword(s):  
Graphene Oxide ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Graphene Nanosheets ◽  
Biological Activities ◽  
Bioactive Molecules ◽  
Dependent Manner ◽  
Tridax Procumbens ◽  
Reduced Graphene

Graphene oxide nanoparticles have found immense application in biotherapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide (rGO) using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized TP-rGO was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic primarily detected the absorption peak from 232 to 287 nm. XRD spectra showed diffraction peak 2θ at 25o, confirming the presence of reduced GO. FTIR and Raman spectra confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. EDAX revealed the presence of carbon (74%) and oxygen (24%). TEM analysis revealed the presence of graphene nanosheets structure appearing as a thin sheet stacked with one another. The bioreduced rGO showed an antioxidant effect against DPPH radical observed in a concentration-dependent manner. The TP-rGO nanosheets were confirming the enhanced antibacterial effects against gram-positive pathogens than gram-negative bacteria. Hence, it is highlighted that leaf extract of T. procumbens act as a green, reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.

scholarly journals Green Synthesis of Reduced Graphene Nanosheets Using Leaf Extract of Tridax procumbens and its Potential In-Vitro Biological Activities

2020 ◽  
Vol 2 (1) ◽  
pp. 93
Keyword(s):  
Graphene Oxide ◽  
Green Synthesis ◽  
Reduced Graphene Oxide ◽  
Leaf Extract ◽  
Spectroscopic Analysis ◽  
Graphene Nanosheets ◽  
Biological Activities ◽  
Tridax Procumbens ◽  
Reduced Graphene

Graphene oxide nanoparticles has found immense application in bio therapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized reduced graphene oxide (rGO) was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic analysis primarily detected the shift in the absorption peak from 232 to 287 nm confirming the reduction of TP-rGO. FTIR spectra of TP-rGO confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. Raman spectroscopic analysis identified successful reduction of GO through the presence of D band at 1329 cm-1 and G band at 1577 cm-1. XRD spectra of TP-rGO showed diffraction peak 2θ at 25o confirming the presence of reduced GO. SEM analysis revealed the presence of graphene nanosheets appearing as large aggregated structure. The bioreduced rGO showed anti-oxidant effect against DPPH radical observed in concentration dependent manner. The TP-rGO nanosheets exhibited bactericidal effect against gram positive bacteria: B.subitils (18 mm) and S. epidermis (9 mm) and gram negative bacteria: E.coli (14 mm) and P. aeruginosa (6 mm) at 100 µg/ml confirming the enhanced anti-bacterial effects of TP-rGO against gram positive pathogens. Hence, it is highlighted that leaf extract of Tridax procumbens act as green reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.

Green synthesis of a Cu/reduced graphene oxide/Fe3O4 nanocomposite using Euphorbia wallichii leaf extract and its application as a recyclable and heterogeneous catalyst for the reduction of 4-nitrophenol and rhodamine B

RSC Advances ◽  
2015 ◽  
Vol 5 (111) ◽  
pp. 91532-91543 ◽  
Author(s):  
Monireh Atarod ◽  
Mahmoud Nasrollahzadeh ◽  
S. Mohammad Sajadi
Keyword(s):  
Graphene Oxide ◽  
Green Synthesis ◽  
Reduced Graphene Oxide ◽  
Heterogeneous Catalyst ◽  
Rhodamine B ◽  
Leaf Extract ◽  
Synthesis Method ◽  
Biological Reduction ◽  
Stabilizing Agent ◽  
Reduced Graphene

Herein, we describe a green and eco-friendly synthesis method for preparing a Cu/RGO/Fe3O4 nanocomposite through biological reduction of graphene oxide and Cu2+, Fe3+ ions using Euphorbia wallichii leaf extract as a reducing and stabilizing agent.

scholarly journals Green synthesis of silver nanoparticles using leaf extract from Tabebuia roseoalba and T. pentaphylla

2019 ◽  
pp. 216-222
Author(s):  
Laureen Michelle Houllou ◽  
Robson Antonio Barbosa De Souza ◽  
Carolina Barbosa Malafaia ◽  
Débora Lorrane Montenegro da Paixão ◽  
Alisson Tito Bezerra de Araújo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Biological Activities ◽  
Nanoparticle Synthesis ◽  
Biological Synthesis ◽  
Natural Conditions ◽  
Synthesis Solution

Metal nanoparticles are nanostructures that can be applied to biotechnology because they present different biological activities. Among them, the silver nanoparticles (AgNPs) are known to present antimicrobial activity allowing their application in several areas such as medicine and industry. The biological synthesis of AgNPs is ecologically correct and advantageous techniques. The objective of this work was to evaluate the synthesis of AgNps through the green synthesis using extracts of leaves of Tabebuia roseoalba and T. pentaphylla grown in vivo and in vitro. The nanoparticle synthesis solution was colorimetrically evaluated, and the nanoparticles were physically characterized. The results obtained demonstrate that both extracts of both Tabebuia species tested are able to synthesize AgNPs, however only when cultured under in vivo conditions. These data suggest that photosynthesis under natural conditions promotes the production of metabolites that are essential to green synthesis.

In vitro Antiurolithiatic activity of the leaves and flowers extracts of Paronychia argentea, a plant used in traditional medicine in Algeria

2021 ◽  
pp. 401-411
Author(s):  
Omar Mechraoui ◽  
Ali Imessaoudene ◽  
Mohamed Y. Maiz ◽  
Hicham Banouh ◽  
Lotfi Mouni ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Leaf Extract ◽  
Bioactive Molecules ◽  
Dependent Manner ◽  
Polyphenol Content ◽  
Concentration Dependent Manner ◽  
Calcium Oxalate Crystallization ◽  
Ethanolic Extracts ◽  
Gallic Acid Equivalent

Plants are a large source of new bioactive molecules with therapeutic potentials. However, only a small amount of worldwide plants has been phytochemically investigated. The ethanolic extracts from leaves and flowers of Paronychia argentea were evaluated for their antilithiasic activity in vitro. The effect of extract (0.1, 0.2, 0.3, 1, 2, and 5mg/mL) was studied by the measurement of turbidity in presence or absence of extract at 620nm using UV/Vis spectrophotometer. Total phenol and flavonoid contents were also evaluated. Polyphenol content was found to be more present in the leaves extract (9.29±0.009mg of Gallic acid equivalent (GAE)/g) compared to the flowers extract (5.92±0.14mg GAE/g). Flavonoids content was also found to be more present in the floral extract that is estimated at 0.18±0.01 mg QE/g compared to the flowers extract (0.47±0.0035mg QE/g). For the antilithiasis activity, the results clearly shown that P. argentia extracts inhibited calcium oxalate crystallization by concentration-dependent manner. The maximum percent inhibition of calcium oxalate by flowers extract was found to be 70.97% at 5mg/mL. Further, P. argentea leaf extract has shown antilithiasic properties and may be used for the prevention of kidneys stones. Plants are a large source of new bioactive molecules with therapeutic potentials. However, only a small amount of worldwide plants has been phytochemically investigated. The ethanolic extracts from leaves and flowers of Paronychia argentea were evaluated for their antilithiasic activity in vitro. The effect of extract (0.1, 0.2, 0.3, 1, 2, and 5mg/mL) was studied by the measurement of turbidity in presence or absence of extract at 620 nm using UV/Vis spectrophotometer. Total phenol and flavonoid contents were also evaluated. Polyphenol content was found to be more present in the leaves extract (9.29±0.009mg of Gallic acid equivalent (GAE)/g) compared to the flowers extract (5.92±0.14mg GAE/g). Flavonoids content was also found to be more present in the floral extract that is estimated at 0.18±0.01mg QE/g compared to the flowers extract (0.47±0.0035mg QE/g). For the antilithiasis activity, the results clearly shown that P. argentia extracts inhibited calcium oxalate crystallization by concentration-dependent manner. The maximum percent inhibition of calcium oxalate by flowers extract was found to be 70.97% at 5mg/mL. Further, P. argentea leaf extract has shown antilithiasic properties and may be used for the prevention of kidneys stones.

scholarly journals Green synthesis of graphene nanosheets and their in vitro cytotoxicity against human prostate cancer (DU 145) cell lines

2017 ◽  
Vol 7 ◽  
pp. 184798041770279 ◽  
Author(s):  
Xin Zhu ◽  
Xiaolin Xu ◽  
Feng Liu ◽  
Jizhong Jin ◽  
Lintao Liu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Graphene Nanosheets ◽  
In Vitro Cytotoxicity ◽  
Biological Study ◽  
Citrullus Colocynthis ◽  
Graphene Oxide Nanosheets ◽  
Green Approach ◽  
Reduced Graphene

A simple and green approach for the synthesis of polyphenol-functionalized reduced graphene oxide nanosheets is demonstrated, using leaf extract of Citrullus colocynthis as a deoxygenating agent. The C. colocynthis polyphenols also play a significant role as a stabilizing agent, preventing agglomeration of reduced graphene oxide nanosheets. Cytotoxicity tests showed that the both graphene oxide and Citrullus colocynthis polyphenol-stabilized reduced graphene oxide were toxic to DU145 cells; the cytotoxicity was dose dependent. Hence, C. colocynthis–mediated reduced graphene oxide may be an ideal anticancer material for biological study applications.

scholarly journals Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sajad Bahrami ◽  
Nafiseh Baheiraei ◽  
Mostafa Shahrezaee
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Cranial Bone ◽  
Drying Methods ◽  
Porous Scaffolds ◽  
Alizarin Red ◽  
Reduced Graphene ◽  
Coated Collagen

AbstractA variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.

Eco-friendly foul release coatings based on a novel reduced graphene oxide/Ag nanocomposite prepared by a green synthesis approach

2021 ◽  
Vol 151 ◽  
pp. 106107
Author(s):  
Soolmaz Soleimani ◽  
Ali Jannesari ◽  
Morteza Yousefzadi ◽  
Arash Ghaderi ◽  
Adnan Shahdadi
Keyword(s):  
Graphene Oxide ◽  
Green Synthesis ◽  
Reduced Graphene Oxide ◽  
Reduced Graphene ◽  
Synthesis Approach
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