Green Synthesis, Characterization and Antibacterial Activity of SiO2–ZnO Nanocomposite by Dictyota bartayresiana Extract and Its Cytotoxic Effect on HT29 Cell Line

The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription. apoptosis, β1 integrin, colorectal cancer, laminin 521 (laminin-11), regorafenib, 5-fluorouracil, HT29, ITGB1, LAMA5, YAP, SFN, 14-3-3σ The study was funded by the Russian Science Foundation (Project 17-14-01338).

Download Full-text

The radiosensitizer effect of 2,6-bis-(2,6 dichlorobenzylidene) cyclohexanone as a selective thiol alkylator on ht29 cell line

International Journal of Current Advanced Research ◽

10.24327/ijcar.2017.3131.0201 ◽

2017 ◽

Vol 6 (4) ◽

pp. 3127-3131

Author(s):

Alireza Doroudi ◽

◽

KoroushZarei ◽

Mohammad Ramin Mohammadi ◽

Mahmoud Hashemitmar ◽

...

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Ht29 Cell Line

Download Full-text

Study of the effect of Pioglitazone and Cetuximab on Cancer Stem-like Cellsenriched HT29 cell line

10.21203/rs.3.rs-519047/v1 ◽

2021 ◽

Author(s):

nasim alamdar ◽

kaveh Baghaei ◽

shirin Farivar ◽

Amir Ali Hamidieh ◽

zohreh Saltanatpour

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Cancer Resistance ◽

Cell Preparation ◽

Vimentin Expression ◽

Epithelial Morphology ◽

Resistance To Chemotherapy ◽

Enrichment Methods ◽

Ht29 Cell Line ◽

E Cadherin

Abstract One of the major causes of cancer resistance to chemotherapy has been found to be the presence of Cancer Stem Cells (CSCs) in cancerous tissues. Probably, these cells are the source of cancer and the cause of malignancy and recurrence in the affected population. Therefore, it is possible to target CSCs to treat cancer. Since the percentage of CSCs in the total tumor mass is very low, so studies about these cells depend on their isolation and enrichment methods. Some studies have suggested that EMT induction in population of normal epithelial cells and cancer cells by inhibiting of E-cadherin, protects them against chemotherapy, anticancer and apoptosis drugs, moreover they get characteristics of CSCs. So in order to study CSCs can enrich them by inhibiting of E-cadherin in tumor population.In this study, we tried to examine how the effect of Pioglitazone and Cetuximab, two drugs used in chemotherapy of Colon Cancer, on CSCs enriched HT29 cell line (was called HT29-shE) in which CSCs were enriched with induction of EMT by inhibiting of E-cadherin using shRNA.For this purpose, after cell preparation EMT and CSCs markers in Pioglitazone and Cetuximab treated cells were assessed and compared with untreated cells using flow cytometry, real‐time PCR and microscopic monitoring. The findings showed mesenchymal morphology of HT29-shE changed to epithelial morphology after Pioglitazone and Cetuximab treatment, moreover E-cadherin expression increased and Vimentin expression decreased. In addition, expression of CSC markers (CD133+ and CD44+) were reduced in HT29-shE after treatment.

Download Full-text

Radiobiological effects of multiple vs. single low-doses pre-irradiation on HT29 cell line

Współczesna Onkologia ◽

10.5114/wo.2014.41386 ◽

2014 ◽

Vol 4 ◽

pp. 230-233

Author(s):

Igor Djan ◽

Slavica Solajic ◽

Mihajla Djan ◽

Natasa Vucinic ◽

Dunja Popovic ◽

...

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Low Doses ◽

Radiobiological Effects ◽

Ht29 Cell Line

Download Full-text

Enrichment of cancer stem‐like cells by the induction of epithelial‐mesenchymal transition using lentiviral vector carrying E‐cadherin shRNA in HT29 cell line

Journal of Cellular Physiology ◽

10.1002/jcp.28855 ◽

2019 ◽

Vol 234 (12) ◽

pp. 22935-22946 ◽

Cited By ~ 4

Author(s):

Zohreh Saltanatpour ◽

Behrooz Johari ◽

Akram Alizadeh ◽

Majid Lotfinia ◽

Keivan Majidzadeh‐A ◽

...

Keyword(s):

Cell Line ◽

Epithelial Mesenchymal Transition ◽

Lentiviral Vector ◽

Ht29 Cell ◽

Mesenchymal Transition ◽

Ht29 Cell Line ◽

E Cadherin

Download Full-text

Studying the Influence of Electroporation on HT29 Cell Line Interaction with Fibronectin and Collagen Protein Micro-Patterned Surface

Journal of Physics Conference Series ◽

10.1088/1742-6596/1529/5/052028 ◽

2020 ◽

Vol 1529 ◽

pp. 052028

Author(s):

Hassan Buhari Mamman ◽

Muhammad Mahadi Abdul Jamil ◽

Tengku Nadzlin Tengku Ibrahim ◽

Mohd Helmy Abd Wahab ◽

Johan Mohamad Sharif ◽

...

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Patterned Surface ◽

Collagen Protein ◽

Ht29 Cell Line

Download Full-text

Chemical Composition, Antioxidant and Cytotoxic Activities of Roots and Fruits of Berberis libanotica

Natural Product Communications ◽

10.1177/1934578x1601100523 ◽

2016 ◽

Vol 11 (5) ◽

pp. 1934578X1601100 ◽

Cited By ~ 2

Author(s):

Leina El Hosry ◽

Laurent Boyer ◽

Elnur E. Garayev ◽

Fathi Mabrouki ◽

Sok-Siya Bun ◽

...

Keyword(s):

Chemical Composition ◽

Chlorogenic Acid ◽

Gallic Acid ◽

Cell Line ◽

Antioxidant Activities ◽

Ht29 Cell ◽

Cytotoxic Activities ◽

First Time ◽

Ht29 Cell Line

Fourteen compounds belonging to different chemical classes were characterized in the roots and fruits extracts from Berberis libanotica, using the same HPLC-DAD-MS method. Thirteen were reported, for the first time, from the fruits whereas the roots contained mostly alkaloids of which 3 out of 5 are reported for the first time. Their structures were established on the basis of MS data as gallic acid (1), chlorogenic acid (2), delphinidin (3), oxyacanthine (4), rutin (5), hyperoside (6), berbamine (7), isoquercitrin (8), quercitrin (9), jatrorrhizine (10), palmatine (11), berberine (12), quercetin (13) and luteolin (14). Extracts containing compounds 4 and 7 showed significant cytotoxicity against the HT29 cell line with an IC50 of 12.2–26.1 μg/mL. Fruits extracts, due mostly to compounds 1 and 2, showed potent antioxidant activities with an EC50 of 0.0025-0.019 mg/mL.

Download Full-text