Chemical Composition, Antioxidant and Cytotoxic Activities of Roots and Fruits of Berberis libanotica

Fourteen compounds belonging to different chemical classes were characterized in the roots and fruits extracts from Berberis libanotica, using the same HPLC-DAD-MS method. Thirteen were reported, for the first time, from the fruits whereas the roots contained mostly alkaloids of which 3 out of 5 are reported for the first time. Their structures were established on the basis of MS data as gallic acid (1), chlorogenic acid (2), delphinidin (3), oxyacanthine (4), rutin (5), hyperoside (6), berbamine (7), isoquercitrin (8), quercitrin (9), jatrorrhizine (10), palmatine (11), berberine (12), quercetin (13) and luteolin (14). Extracts containing compounds 4 and 7 showed significant cytotoxicity against the HT29 cell line with an IC50 of 12.2–26.1 μg/mL. Fruits extracts, due mostly to compounds 1 and 2, showed potent antioxidant activities with an EC50 of 0.0025-0.019 mg/mL.

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Laminin 521 Modulates the Сytotoxic Effect of 5-Fluorouracil on Colorectal Cancer HT29 Cells

Biotekhnologiya ◽

10.21519/0234-2758-2019-35-6-73-79 ◽

2019 ◽

Vol 35 (6) ◽

pp. 73-79

Author(s):

A. Turchinovich ◽

I.M. Tsypina ◽

V.G. Zgoda ◽

S.V. Nikulin ◽

D.V. Maltseva

Keyword(s):

Colorectal Cancer ◽

Cytotoxic Effect ◽

Mechanisms Of Action ◽

Ht29 Cell ◽

Β1 Integrin ◽

Russian Science ◽

Apoptotic Gene ◽

Ht29 Cells ◽

First Time ◽

Ht29 Cell Line

The cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF)-drugs with different mechanisms of action used to treat colorectal cancer-on the HT29 cell line when cultured on plastic and laminin 521 (LM-521) has been studied. It was shown for the first time that LM-521 can increase the sensitivity of tumor cells to 5FU. Based on the analysis of the transcriptome and proteome, a possible mechanism of the observed effect of LM-521 on HT29 cell viability was proposed. The interaction of β1-containing integrins on the cell surface with LM-521 can activate the FAK/PI3K/Akt signaling pathways, promote phosphorylation of the YAP transcription coactivator and its binding to a complex with the 14-3-3σ protein. The formation of such complex leads to the YAP retention in the cytoplasm, prevents its transport to the nucleus and the activation of anti-apoptotic gene transcription. apoptosis, β1 integrin, colorectal cancer, laminin 521 (laminin-11), regorafenib, 5-fluorouracil, HT29, ITGB1, LAMA5, YAP, SFN, 14-3-3σ The study was funded by the Russian Science Foundation (Project 17-14-01338).

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The radiosensitizer effect of 2,6-bis-(2,6 dichlorobenzylidene) cyclohexanone as a selective thiol alkylator on ht29 cell line

International Journal of Current Advanced Research ◽

10.24327/ijcar.2017.3131.0201 ◽

2017 ◽

Vol 6 (4) ◽

pp. 3127-3131

Author(s):

Alireza Doroudi ◽

◽

KoroushZarei ◽

Mohammad Ramin Mohammadi ◽

Mahmoud Hashemitmar ◽

...

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Ht29 Cell Line

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Study of the effect of Pioglitazone and Cetuximab on Cancer Stem-like Cellsenriched HT29 cell line

10.21203/rs.3.rs-519047/v1 ◽

2021 ◽

Author(s):

nasim alamdar ◽

kaveh Baghaei ◽

shirin Farivar ◽

Amir Ali Hamidieh ◽

zohreh Saltanatpour

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Cancer Resistance ◽

Cell Preparation ◽

Vimentin Expression ◽

Epithelial Morphology ◽

Resistance To Chemotherapy ◽

Enrichment Methods ◽

Ht29 Cell Line ◽

E Cadherin

Abstract One of the major causes of cancer resistance to chemotherapy has been found to be the presence of Cancer Stem Cells (CSCs) in cancerous tissues. Probably, these cells are the source of cancer and the cause of malignancy and recurrence in the affected population. Therefore, it is possible to target CSCs to treat cancer. Since the percentage of CSCs in the total tumor mass is very low, so studies about these cells depend on their isolation and enrichment methods. Some studies have suggested that EMT induction in population of normal epithelial cells and cancer cells by inhibiting of E-cadherin, protects them against chemotherapy, anticancer and apoptosis drugs, moreover they get characteristics of CSCs. So in order to study CSCs can enrich them by inhibiting of E-cadherin in tumor population.In this study, we tried to examine how the effect of Pioglitazone and Cetuximab, two drugs used in chemotherapy of Colon Cancer, on CSCs enriched HT29 cell line (was called HT29-shE) in which CSCs were enriched with induction of EMT by inhibiting of E-cadherin using shRNA.For this purpose, after cell preparation EMT and CSCs markers in Pioglitazone and Cetuximab treated cells were assessed and compared with untreated cells using flow cytometry, real‐time PCR and microscopic monitoring. The findings showed mesenchymal morphology of HT29-shE changed to epithelial morphology after Pioglitazone and Cetuximab treatment, moreover E-cadherin expression increased and Vimentin expression decreased. In addition, expression of CSC markers (CD133+ and CD44+) were reduced in HT29-shE after treatment.

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Bioactive Phenolics from Carthamus lanatus L.

Zeitschrift für Naturforschung C ◽

10.1515/znc-2003-9-1019 ◽

2003 ◽

Vol 58 (9-10) ◽

pp. 704-707 ◽

Cited By ~ 11

Author(s):

Rilka Taskova ◽

Maya Mitova ◽

Bozhanka Mikhova ◽

Helmut Duddeck

Keyword(s):

Chlorogenic Acid ◽

Methanol Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Free Radical Scavenging ◽

Flavonoid Glycosides ◽

Aerial Parts ◽

Free Radical Scavenging Assay ◽

First Time

AbstractTwo flavonoid aglycons, eight flavonoid glycosides, chlorogenic acid and syringin were isolated from aerial parts of Carthamus lanatus. Isorhamnetin 3-O-β-ᴅ-glucoside and chlorogenic acid were found for the first time in the genus Carthamus and respectively, quercimeritrin, astragalin, kaempferol 3-O-β-ᴅ-sophoroside and syringin in the species. The ethyl acetate fraction of the methanol extract exhibited a higher antioxidant activity than the butanol fraction measured by the α,α-diphenyl-β-picrazylhydrazyl (DPPH) free radical scavenging assay. Cytotoxicity and antioxidant activities of the main constituent, luteolin 7-O-β-D-glucoside, were evaluated.

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Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

Journal of Apicultural Science ◽

10.2478/jas-2013-0025 ◽

2013 ◽

Vol 57 (2) ◽

pp. 147-157 ◽

Cited By ~ 24

Author(s):

Renata Markiewicz-Żukowska ◽

Sylwia K. Naliwajko ◽

Emilia Bartosiuk ◽

Justyna Moskwa ◽

Valery Isidorov ◽

...

Keyword(s):

Chemical Composition ◽

Cell Line ◽

Phenolic Content ◽

Inhibitory Effect ◽

Total Phenolic ◽

Cytotoxic Activities ◽

Glioblastoma Cell Line ◽

Glioblastoma Cell ◽

Acid Fermentation

Abstract Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 μg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.

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Radiobiological effects of multiple vs. single low-doses pre-irradiation on HT29 cell line

Współczesna Onkologia ◽

10.5114/wo.2014.41386 ◽

2014 ◽

Vol 4 ◽

pp. 230-233

Author(s):

Igor Djan ◽

Slavica Solajic ◽

Mihajla Djan ◽

Natasa Vucinic ◽

Dunja Popovic ◽

...

Keyword(s):

Cell Line ◽

Ht29 Cell ◽

Low Doses ◽

Radiobiological Effects ◽

Ht29 Cell Line

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Comparative Study of the Chemical Compositions and Antioxidant Activities of Fresh Juices from Romanian Cucurbitaceae Varieties

Molecules ◽

10.3390/molecules25225468 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5468

Author(s):

Anna Cazanevscaia Busuioc ◽

Andreea-Veronica Dediu Botezatu ◽

Bianca Furdui ◽

Costel Vinatoru ◽

Filippo Maggi ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

High Performance ◽

Antioxidant Activities ◽

Fruit Juices ◽

Antioxidant Potential ◽

Chemical Compositions ◽

Beneficial Effects ◽

Benincasa Hispida ◽

First Time

Cucurbitaceae is a family of health-promoting plants due to their compounds with beneficial effects. The aim of this study was to analyze, for the first time, the chemical composition, the antioxidant activity and the metal chelating properties of fruit juices obtained from four different species of the Cucurbitaceae family cultivated in Romania, namely Momordica charantia, Cucumis metuliferus, Benincasa hispida and Trichosanthes cucumerina. The samples of juice were analyzed by high-performance liquid chromatography (HPLC) and all the four species displayed high levels of the two triterpenes, oleanolic and ursolic acids, and also in phenolic compounds, including catechin, (−)-epicatechin and gallic acid. The juices demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (ranging from 20 to 95%,), a good iron binding ability (ranging from 7.45 ± 0.28% to 86.95 ± 0.97%) and also promising antioxidant potential against the ABTS radical (ranging from 4.97 to 32.60 μETx/mL juice). Our findings raise interesting questions for further research on Cucurbitaceae fruit juices and, consequently, their very good antioxidant potential suggests these fruits should be further explored for their protective effect against oxidative damage. This is the first time the chemical composition and antioxidant activities of fruit juices from these four Romanian Cucurbitaceae varieties have been investigated.

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Chemical Composition, Antifungal, and Cytotoxicity Activities of Inga laurina (Sw.) Willd Leaves

The Scientific World JOURNAL ◽

10.1155/2019/9423658 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Carla de Moura Martins ◽

Sérgio A. L. de Morais ◽

Mário M. Martins ◽

Luís C. S. Cunha ◽

Cláudio V. da Silva ◽

...

Keyword(s):

Chemical Composition ◽

Gallic Acid ◽

Total Phenolics ◽

Biological Activities ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Vero Cells ◽

Quercetin Glycosides ◽

Cytotoxicity Activities ◽

First Time

The species Inga laurina is native to the Brazilian Cerrado. There are no studies about the chemical composition and biological activities of extracts of this endangered species. The ethanolic extract and its successive fractions are rich in phenolic compounds and presented good antifungal activities. HPLC/MS-MS/MS and H1/C13 analysis led to the identification of seventeen compounds, most of which are gallic acid derivatives, myricetin and quercetin glycosides. The ethyl acetate fraction (EAF) contained high levels of total phenolics, expressed in milligrams of gallic acid equivalents per gram of extract (475.3 ± 1.9 mg GAE gextract-1) and flavonoids expressed in milligrams of quercetin equivalents per gram of extract (359.3 ± 10.6 mg QE gextract-1). This fraction was active against fungi of the Candida genus. The EAF showed MIC value 11.7 μg mL−1 against C. glabrata and a selectivity index of 1.6 against Vero cells. The flavonol glycoside myricetin-3-O-rhamnoside was isolated for the first time from the Inga laurina. These results make I. laurina a promising plant as a source of pharmaceutical and biological active antifungal compounds.

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