Propofol protects against hydrogen peroxide-induced oxidative stress and cell dysfunction in human umbilical vein endothelial cells

Vestigial-like 4 (VGLL4) has been found to have multiple functions in tumor development; however, its role in cardiovascular disease is unknown. The aim of this study was to investigate the effect of VGLL4 on the dysfunction and inflammatory response of Ox-LDL-induced human umbilical vein endothelial cells (HUVECs) and its mechanism, so as to provide a new theoretical basis for the diagnosis and treatment of atherosclerosis. In the present study, the protective activity of VGLL4 inhibiting Ox-LDL-induced apoptosis, oxidative stress, inflammation, and injury as well as its molecular mechanisms was examined using human umbilical vein endothelial cells (HUVECs). The results showed that the expression of VGLL4 was decreased with the increase of Ox-LDL concentration in HUVECs. In addition, the functional study found that VGLL4 overexpression alleviated Ox-LDL-induced oxidative stress, inflammation, and dysfunction and inhibited apoptosis. Further research found that VGLL4 regulated Hippo-YAP/TEAD1 signaling pathway, and the Hippo-YAP/TEAD1 signaling pathway was involved in the protective mechanism of VGLL4 on HUVECs. In conclusion, it suggests that VGLL4 protects against oxidized-LDL-induced endothelial cell dysfunction by activating the Hippo-YAP/TEAD1 signaling pathway.

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Protective effects of peoniflorin against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y11-034 ◽

2011 ◽

Vol 89 (6) ◽

pp. 445-453 ◽

Cited By ~ 34

Author(s):

Tao Chen ◽

Zai-pei Guo ◽

Xiao-yan Jiao ◽

Yu-hong Zhang ◽

Jing-yi Li ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Endothelial Cells ◽

Oxidative Damage ◽

Umbilical Vein ◽

Flow Cytometric Analysis ◽

Vascular Diseases ◽

Protective Effects ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

Peoniflorin (PF), extracted from the root of Paeonia lactiflora Pall., has been reported to have anti-inflammation and antioxidant effects in several animal models. Herein, we investigated the protective effects of PF against hydrogen peroxide (H2O2)-induced oxidative damage in human umbilical vein endothelial cells (HUVECs). HUVECs were treated by H2O2 (240 µmol/L) with or without PF. PF significantly increased the percent cell viability of HUVECs injured by H2O2 using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. By flow cytometric analysis, PF markedly attenuated H2O2-induced apoptosis and intracellular reactive oxygen species production. In addition, PF also displayed a dose-dependent reduction of lactate dehydrogenase leakage, malondialdehyde formation, and caspase-3 proteolytic activities in H2O2-treated cells, which was accompanied with a restoration of the activities of endogenous antioxidants, including total superoxide dismutase and glutathione peroxidase. Finally, Western blot data revealed that H2O2 upregulated phosphorylation of extracellular signal-regulated kinase 1/2 in HUVECs, which was almost completely reversed by PF. Taken together, our data provide the first evidence that PF has a protective ability against oxidative damage in HUVECs. PF may be a candidate medicine for the treatment of vascular diseases associated with oxidative stress.

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Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide

Journal of Zhejiang University SCIENCE B ◽

10.1631/jzus.b0900397 ◽

2010 ◽

Vol 11 (5) ◽

pp. 357-365 ◽

Cited By ~ 24

Author(s):

Abdul Hamid Hafizah ◽

Zakaria Zaiton ◽

Amom Zulkhairi ◽

Adenan Mohd Ilham ◽

Megat Mohd Nordin Nor Anita ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Endothelial Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

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Zhenbao pill attenuates hydrogen peroxide–induced apoptosis by inhibiting autophagy in human umbilical vein endothelial cells

Journal of Ethnopharmacology ◽

10.1016/j.jep.2021.114020 ◽

2021 ◽

pp. 114020

Author(s):

Yuchen Jia ◽

Xiaoxue Chen ◽

Yajing Chen ◽

Hongxia Li ◽

Xiumei Ma ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Endothelial Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

Induced Apoptosis ◽

Umbilical Vein Endothelial Cells

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Cytoprotective Role of Edible Seahorse (Hippocampus abdominalis)-Derived Peptides in H2O2-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells

Marine Drugs ◽

10.3390/md19020086 ◽

2021 ◽

Vol 19 (2) ◽

pp. 86

Author(s):

Yunok Oh ◽

Chang-Bum Ahn ◽

Jae-Young Je

Keyword(s):

Oxidative Stress ◽

Endothelial Cells ◽

Cardiovascular Diseases ◽

Combination Treatment ◽

Umbilical Vein ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Nuclear Staining ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

Oxidative stress-induced endothelial dysfunction is strongly linked to the pathogenesis of cardiovascular diseases. A previous study revealed that seahorse hydrolysates ameliorated oxidative stress-mediated human umbilical vein endothelial cells (HUVECs) injury. However, the responsible compounds have not yet been identified. This study aimed to identify cytoprotective peptides and to investigate the molecular mechanism underlying the cytoprotective role in H2O2-induced HUVECs injury. After purification by gel filtration and HPLC, two peptides were sequenced by liquid chromatography-tandem mass spectrometry as HGSH (436.43 Da) and KGPSW (573.65 Da). The synthesized peptides and their combination (1:1 ratio) showed significant HUVECs protection effect at 100 μg/mL against H2O2-induced oxidative damage via significantly reducing intracellular reactive oxygen species (ROS). Two peptides and their combination treatment resulted in the increased heme oxygenase-1 (HO-1), a phase II detoxifying enzyme, through the activation of nuclear transcription factor-erythroid 2-related factor (Nrf2). Additionally, cell cycle and nuclear staining analysis revealed that two peptides and their combination significantly protected H2O2-induced cell death through antiapoptotic action. Two peptides and their combination treatment led to inhibit the expression of proapoptotic Bax, the release of cytochrome C into the cytosol, the activation of caspase 3 by H2O2 treatment in HUVECs, whereas antiapoptotic Bcl-2 expression was increased with concomitant downregulation of Bax/Bcl-2 ratio. Taken together, these results suggest that seahorse-derived peptides may be a promising agent for oxidative stress-related cardiovascular diseases.

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