Short-term serum supplementation improves glucose-oxygen deprivation in primary cortical cultures grown under serum-free conditions

2004 ◽  
Vol 25 (3-4) ◽  
pp. 227-236
Author(s):  
Jan Lewerenz ◽  
Susanne Thomsen ◽  
Julius A. Steinbeck ◽  
Axel Methner
Keyword(s):  
Oxygen Deprivation ◽  
Short Term ◽  
Serum Free ◽  
Serum Supplementation ◽  
Cortical Cultures ◽  
Serum Free Conditions

scholarly journals Development of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free Conditions

2016 ◽  
Vol 11 (2) ◽  
pp. 61-69 ◽  
Author(s):  
Francesca Abramo ◽  
Andrea Pirone ◽  
Carla Lenzi ◽  
Maria Federica della Valle ◽  
Silvia Vidali ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Full Thickness ◽  
Short Term ◽  
Serum Free ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Serum Free Conditions ◽  
Skin Organ Culture

Effects of recombinant bovine somatotrophin, insulin-like growth factor-I and insulin on bovine granulosa cell steroidogenesis in vitro

1994 ◽  
Vol 143 (1) ◽  
pp. 157-164 ◽  
Author(s):  
J G Gong ◽  
D McBride ◽  
T A Bramley ◽  
R Webb
Keyword(s):  
Granulosa Cell ◽  
Granulosa Cells ◽  
Dependent Manner ◽  
Serum Free ◽  
Factor I ◽  
Igf I ◽  
Serum Free Conditions ◽  
Dose Dependent ◽  
Bovine Somatotrophin ◽  
Cells Cultured

Abstract Our previous studies have demonstrated that physiological concentrations of metabolic hormones, including recombinant bovine somatotrophin (BST), insulin-like growth factor-I (IGF-I) and insulin, can significantly stimulate the proliferation of bovine granulosa cells cultured under serum-free conditions. In this study we investigated the effects of these factors on bovine granulosa cell steroidogenesis using the same culture system. Bovine granulosa cells were obtained from antral follicles classified into three size classes: small, <5 mm; medium-sized, 5–10 mm and large, >10 mm in diameter. Whilst not affecting steroidogenesis by granulosa cells from small and medium-sized follicles, BST (10–1000 ng/ml) stimulated the secretion of both oestradiol and progesterone by granulosa cells from large follicles in a dose-dependent manner. Insulin (1–1000 ng/ml) and IGF-I (10–1000 ng/ml) stimulated the secretion of oestradiol and progesterone by granulosa cells from all three size categories of follicles in a dose-dependent manner. FSH (200 ng/ml) alone increased progesterone secretion by granulosa cells from all three size classes of follicles, but had no effect on oestradiol secretion by granulosa cells. Both IGF-I (200 ng/ml) and insulin (30 ng/ml) acted in synergy with FSH (200 ng/ml) to stimulate steroidogenesis by granulosa cells from all three size categories of follicles, but no such interaction was observed between BST (50 ng/ml) and FSH (200 ng/ml). In conclusion, BST, IGF-I and insulin significantly influence the steroidogenic activity of bovine granulosa cells cultured under serum-free conditions. However, unlike their effects on cell proliferation, the minimal effective concentrations of these factors required to stimulate granulosa cell steroidogenesis were higher than those observed in our previous studies in vivo. Journal of Endocrinology (1994) 143, 157–164

Immunomodulatory effects of rhesus monkey bone marrow-derived mesenchymal stem cells in serum-free conditions

2018 ◽  
Vol 64 ◽  
pp. 364-371 ◽  
Author(s):  
Fei Liu ◽  
Yuanmin Li ◽  
Lin Bai ◽  
Zhen Yang ◽  
Guangneng Liao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Rhesus Monkey ◽  
Immunomodulatory Effects ◽  
Serum Free ◽  
Serum Free Conditions
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