Effect of simultaneous down-regulation of pectate lyase and endo-β-1,4-glucanase genes on strawberry fruit softening

2012 ◽  
Vol 31 (2) ◽  
pp. 313-322 ◽  
Author(s):  
Sabry M. Youssef ◽  
Iraida Amaya ◽  
José M. López-Aranda ◽  
Rafael Sesmero ◽  
Victoriano Valpuesta ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Down Regulation

scholarly journals Manipulation of Strawberry Fruit Softening by Antisense Expression of a Pectate Lyase Gene

2002 ◽  
Vol 128 (2) ◽  
pp. 751-759 ◽  
Author(s):  
Silvia Jiménez-Bermúdez ◽  
José Redondo-Nevado ◽  
Juan Muñoz-Blanco ◽  
José L. Caballero ◽  
José M. López-Aranda ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Antisense Expression

scholarly journals Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening

2008 ◽  
Vol 59 (10) ◽  
pp. 2769-2779 ◽  
Author(s):  
N. Santiago-Domenech ◽  
S. Jimenez-Bemudez ◽  
A. J. Matas ◽  
J. K. C. Rose ◽  
J. Munoz-Blanco ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Antisense Inhibition ◽  
Strawberry Fruit

scholarly journals Antisense Down-Regulation of the FaPG1 Gene Reveals an Unexpected Central Role for Polygalacturonase in Strawberry Fruit Softening

2009 ◽  
Vol 150 (2) ◽  
pp. 1022-1032 ◽  
Author(s):  
Miguel A. Quesada ◽  
Rosario Blanco-Portales ◽  
Sara Posé ◽  
Juan A. García-Gago ◽  
Silvia Jiménez-Bermúdez ◽  
...  
Keyword(s):  
Fruit Softening ◽  
Strawberry Fruit ◽  
Down Regulation

scholarly journals Sensing when the wall comes tumbling down

2020 ◽  
Vol 71 (22) ◽  
pp. 6865-6868
Author(s):  
David A Brummell
Keyword(s):  
Fruit Softening ◽  
Strawberry Fruit ◽  
Experimental Botany

This article comments on: Paniagua C, Ric-Varas P, Garcia-Gago JA, López-Casado G, Blanco-Portales R, Muñoz-Blanco J, Schückel J, Knox JP, Matas AJ, Quesada MA, Posé S, Mercado JA. 2020. Elucidating the role of polygalacturonase genes in strawberry fruit softening. Journal of Experimental Botany 71, 7103–7117.

scholarly journals Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

2020 ◽  
Vol 71 (12) ◽  
pp. 3560-3574 ◽  
Author(s):  
Ying Gao ◽  
Wei Wei ◽  
Zhongqi Fan ◽  
Xiaodan Zhao ◽  
Yiping Zhang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Tomato Fruit ◽  
Pectate Lyase ◽  
Ethylene Biosynthesis ◽  
Fruit Softening ◽  
Wild Type ◽  
Gain Of Function ◽  
Carotenoid Accumulation ◽  
Acid Protein ◽  
Tomato Fruit Ripening

Abstract The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be a gain-of-function mutant. Here, we provide more evidence to support this view and answer the open question of whether the NAC-NOR gene is important in fruit ripening. Overexpression of NAC-NOR in the nor mutant did not restore the full ripening phenotype. Further analysis showed that the truncated NOR186 protein is located in the nucleus and binds to but does not activate the promoters of 1-aminocyclopropane-1-carboxylic acid synthase2 (SlACS2), geranylgeranyl diphosphate synthase2 (SlGgpps2), and pectate lyase (SlPL), which are involved in ethylene biosynthesis, carotenoid accumulation, and fruit softening, respectively. The activation of the promoters by the wild-type NOR protein can be inhibited by the mutant NOR186 protein. On the other hand, ethylene synthesis, carotenoid accumulation, and fruit softening were significantly inhibited in CR-NOR (CRISPR/Cas9-edited NAC-NOR) fruit compared with the wild-type, but much less severely affected than in the nor mutant, while they were accelerated in OE-NOR (overexpressed NAC-NOR) fruit. These data further indicated that nor is a gain-of-function mutation and NAC-NOR plays a significant role in ripening of wild-type fruit.

EFFECT OF SILENCING OF CELL WALL DEGRADING ENZYMES ON STRAWBERRY FRUIT SOFTENING

2009 ◽  
pp. 931-934 ◽  
Author(s):  
M.A. Quesada ◽  
S. Posé ◽  
N. Santiago-Doménech ◽  
R. Sesmero ◽  
M.C. Molina ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes
Sign in / Sign up

Export Citation Format

Share Document