Strawberry Fruit Softening: The Potential Roles of Polyuronides and Hemicelluloses

1984 ◽  
Vol 49 (5) ◽  
pp. 1310-1315 ◽  
Author(s):  
D. J. HUBER
Keyword(s):  
Fruit Softening ◽  
Strawberry Fruit

scholarly journals Sensing when the wall comes tumbling down

2020 ◽  
Vol 71 (22) ◽  
pp. 6865-6868
Author(s):  
David A Brummell
Keyword(s):  
Fruit Softening ◽  
Strawberry Fruit ◽  
Experimental Botany

This article comments on: Paniagua C, Ric-Varas P, Garcia-Gago JA, López-Casado G, Blanco-Portales R, Muñoz-Blanco J, Schückel J, Knox JP, Matas AJ, Quesada MA, Posé S, Mercado JA. 2020. Elucidating the role of polygalacturonase genes in strawberry fruit softening. Journal of Experimental Botany 71, 7103–7117.

EFFECT OF SILENCING OF CELL WALL DEGRADING ENZYMES ON STRAWBERRY FRUIT SOFTENING

2009 ◽  
pp. 931-934 ◽  
Author(s):  
M.A. Quesada ◽  
S. Posé ◽  
N. Santiago-Doménech ◽  
R. Sesmero ◽  
M.C. Molina ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Cell Wall Degrading Enzymes ◽  
Degrading Enzymes

scholarly journals Manipulation of Strawberry Fruit Softening by Antisense Expression of a Pectate Lyase Gene

2002 ◽  
Vol 128 (2) ◽  
pp. 751-759 ◽  
Author(s):  
Silvia Jiménez-Bermúdez ◽  
José Redondo-Nevado ◽  
Juan Muñoz-Blanco ◽  
José L. Caballero ◽  
José M. López-Aranda ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Antisense Expression

scholarly journals FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38

2021 ◽  
Vol 23 (1) ◽  
pp. 101
Author(s):  
Jianfa Cai ◽  
Xuelian Mo ◽  
Chenjin Wen ◽  
Zhen Gao ◽  
Xu Chen ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Transcriptional Activation ◽  
Developmental Stages ◽  
Pectin Methylesterase ◽  
Myb Transcription Factor ◽  
Transcriptional Level ◽  
Fruit Softening ◽  
Fruit Firmness ◽  
Strawberry Fruit ◽  
Softening Process

Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role of FvPME38 in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level of FvPME38, thereby accelerating fruit softening. During fruit development, FvMYB79 co-expressed with FvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of ‘Ruegen’ which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level of FvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing of FvMYB79 showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement of FvMYB79 in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation of FvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.

scholarly journals Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Cheng Xue ◽  
Si-Cong Guan ◽  
Jian-Qing Chen ◽  
Chen-Jin Wen ◽  
Jian-Fa Cai ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruit Ripening ◽  
Genetic Manipulation ◽  
Functional Characterization ◽  
Hydrolytic Enzyme ◽  
Wall Structure ◽  
Fruit Softening ◽  
Fruit Firmness ◽  
Strawberry Fruit ◽  
Cell Wall Modification

Abstract Background Pectin methylesterase (PME) is a hydrolytic enzyme that catalyzes the demethylesterification of homogalacturonans and controls pectin reconstruction, being essential in regulation of cell wall modification. During fruit ripening stage, PME-mediated cell wall remodeling is an important process to determine fruit firmness and softening. Strawberry fruit is a soft fruit with a short postharvest life, due to a rapid loss of firm texture. Hence, preharvest improvement of strawberry fruit rigidity is a prerequisite for extension of fruit refreshing time. Although PME has been well characterized in model plants, knowledge regarding the functionality and evolutionary property of PME gene family in strawberry remain limited. Results A total of 54 PME genes (FvPMEs) were identified in woodland strawberry (Fragaria vesca ‘Hawaii 4’). Phylogeny and gene structure analysis divided these FvPME genes into four groups (Group 1–4). Duplicate events analysis suggested that tandem and dispersed duplications effectively contributed to the expansion of the PME family in strawberry. Through transcriptome analysis, we identified FvPME38 and FvPME39 as the most abundant-expressed PMEs at fruit ripening stages, and they were positively regulated by abscisic acid. Genetic manipulation of FvPME38 and FvPME39 by overexpression and RNAi-silencing significantly influences the fruit firmness, pectin content and cell wall structure, indicating a requirement of PME for strawberry fruit softening. Conclusion Our study globally analyzed strawberry pectin methylesterases by the approaches of phylogenetics, evolutionary prediction and genetic analysis. We verified the essential role of FvPME38 and FvPME39 in regulation of strawberry fruit softening process, which provided a guide for improving strawberry fruit firmness by modifying PME level.

Study of the structure and binding site features of FaEXPA2, an α-expansin protein involved in strawberry fruit softening

2020 ◽  
Vol 87 ◽  
pp. 107279 ◽  
Author(s):  
Felipe Valenzuela-Riffo ◽  
Luis Morales-Quintana
Keyword(s):  
Binding Site ◽  
Fruit Softening ◽  
Strawberry Fruit

scholarly journals Antisense inhibition of a pectate lyase gene supports a role for pectin depolymerization in strawberry fruit softening

2008 ◽  
Vol 59 (10) ◽  
pp. 2769-2779 ◽  
Author(s):  
N. Santiago-Domenech ◽  
S. Jimenez-Bemudez ◽  
A. J. Matas ◽  
J. K. C. Rose ◽  
J. Munoz-Blanco ◽  
...  
Keyword(s):  
Pectate Lyase ◽  
Fruit Softening ◽  
Antisense Inhibition ◽  
Strawberry Fruit

Evaluation of the role of the endo-β-(1,4)-glucanase gene FaEG3 in strawberry fruit softening

2010 ◽  
Vol 55 (1) ◽  
pp. 8-14 ◽  
Author(s):  
José A. Mercado ◽  
Livio Trainotti ◽  
Lara Jiménez-Bermúdez ◽  
Nieves Santiago-Doménech ◽  
Sara Posé ◽  
...  
Keyword(s):  
Fruit Softening ◽  
Strawberry Fruit

UV-C irradiation delays strawberry fruit softening and modifies the expression of genes involved in cell wall degradation

2009 ◽  
Vol 51 (2) ◽  
pp. 141-148 ◽  
Author(s):  
Marina A. Pombo ◽  
Marcela C. Dotto ◽  
Gustavo A. Martínez ◽  
Pedro M. Civello
Keyword(s):  
Cell Wall ◽  
Fruit Softening ◽  
Strawberry Fruit ◽  
Cell Wall Degradation ◽  
Expression Of Genes ◽  
Uv C
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