Molecular and phenotypic characterization of seedling and adult plant leaf rust resistance in a world wheat collection

Among the rust diseases, leaf rust of wheat caused by Puccinia triticina, is the most prevalent worldwide and causes significant yield losses. This study aimed to determine the genomic location of loci that control adult plant resistance (APR) to leaf rust in the pre-Green Revolution landrace accession, Aus27506, from the “Watkins Collection”. An Aus27506/Aus27229-derived F7 recombinant inbred line (RIL) population was screened under field conditions across three cropping seasons and genotyped with the iSelect 90K Infinium SNP bead chip array. One quantitative trait loci (QTL) on each of the chromosomes 1BL, 2B and 2DL explained most of the leaf rust response variation in the RIL population, and these were named QLr.sun-1BL, QLr.sun-2B and QLr.sun-2DL, respectively. QLr.sun-1BL and QLr.sun-2DL were contributed by Aus27506. QLr.sun-1BL is likely Lr46, while QLr.sun-2DL appeared to be a new APR locus. The alternate parent, Aus27229, carried the putatively new APR locus QLr.sun-2B. The comparison of average severities among RILs carrying these QTL in different combinations indicated that QLr.sun-2B does not interact with either of the other two QTL; however, the combination of QLr.sun-1BL and QLr.sun-2DL reduced disease severity significantly. In planta fungal quantification assays validated these results. The RILs carrying QLr.sun-1BL and QLr.sun-2DL did not differ significantly from the parent Aus27506 in terms of resistance. Aus27506 can be used as a source of adult plant leaf rust resistance in breeding programs.

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Transfer to hexaploid wheat of linked genes for adult-plant leaf rust and seedling stem rust resistance from an amphiploid of Aegilops speltoides × Triticum monococcum

Genome ◽

10.1139/g90-079 ◽

1990 ◽

Vol 33 (4) ◽

pp. 530-537 ◽

Cited By ~ 70

Author(s):

E. R. Kerber ◽

P. L. Dyck

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Dominant Gene ◽

Leaf Rust Resistance ◽

Stem Rust Resistance ◽

Adult Plant ◽

Aegilops Speltoides ◽

Plant Leaf

A partially dominant gene for adult-plant leaf rust resistance together with a linked, partially dominant gene for stem rust resistance were transferred to the hexaploid wheat cultivar 'Marquis' from an amphiploid of Aegilops speltoides × Triticum monococcum by direct crossing and backcrossing. Pathological evidence indicated that the alien resistance genes were derived from Ae. speltoides. Differential transmission of the resistance genes through the male gametes occurred in hexaploid hybrids involving the resistant 'Marquis' stock and resulted in distorted segregation ratios. In heterozygotes, pairing between the chromosome arm with the alien segment and the corresponding arm of the normal wheat chromosome was greatly reduced. The apparent close linkage between the two resistance genes, 3 ± 1.07 crossover units, was misleading because of this decrease in pairing in the presence of the 5B diploidizing mechanism. The newly identified gene for adult-plant leaf rust resistance, located on chromosome 2B, is different from adult-plant resistance genes Lr12, Lr13, and Lr22 and from that in the hexaploid accession PI250413; it has been designated Lr35. It is not known whether the newly transferred gene for stem rust resistance differs from Sr32, also derived from Ae. speltoides and located on chromosomes 2B.Key words: hexaploid, Triticum, Aegilops, aneuploid, Puccinia graminis, Puccinia recondita.

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Novel complementary genes for adult plant leaf rust resistance in a wheat stock carrying the 1BL‐1RS translocation

Plant Breeding ◽

10.1046/j.1439-0523.1999.118003269.x ◽

1999 ◽

Vol 118 (3) ◽

pp. 269-271 ◽

Cited By ~ 7

Author(s):

R. N. Sawhney ◽

J. B. Sharma

Keyword(s):

Leaf Rust ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Plant Leaf ◽

Complementary Genes

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Genetic analysis of adult plant leaf rust resistance in three bread wheat (Triticum aestivum L.) cultivars

Euphytica ◽

10.1007/s10681-006-9271-8 ◽

2006 ◽

Vol 154 (1-2) ◽

pp. 75-82

Author(s):

D. Datta ◽

M. Prashar ◽

S. C. Bhardwaj ◽

S. Kumar

Keyword(s):

Triticum Aestivum ◽

Genetic Analysis ◽

Leaf Rust ◽

Bread Wheat ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Triticum Aestivum L ◽

Adult Plant ◽

Plant Leaf

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Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS

Theoretical and Applied Genetics ◽

10.1007/s00122-014-2285-z ◽

2014 ◽

Vol 127 (5) ◽

pp. 1133-1141 ◽

Cited By ~ 10

Author(s):

M. J. Diéguez ◽

M. F. Pergolesi ◽

S. M. Velasquez ◽

L. Ingala ◽

M. López ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Fine Mapping ◽

Rust Resistance ◽

Wheat Chromosome ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Adult Plant ◽

Leaf Rust Resistance Gene ◽

Plant Leaf

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Mapping of Adult Plant Leaf Rust Resistance in Aus27506 and Validation of Underlying Loci by In-Planta Fungal Biomass Accumulation

10.20944/preprints202004.0487.v1 ◽

2020 ◽

Author(s):

Pakeerathan Kandiah ◽

Mumta Chhetri ◽

Matthew Hayden ◽

Michael Ayliffe ◽

Harbans Bariana ◽

...

Keyword(s):

Leaf Rust ◽

Rust Resistance ◽

Green Revolution ◽

Puccinia Triticina ◽

Biomass Accumulation ◽

Leaf Rust Resistance ◽

Adult Plant ◽

In Planta ◽

Plant Leaf ◽

Ril Population

Among the rust diseases, leaf rust of wheat caused by Puccinia triticina, is the most prevalent worldwide and causes significant yield losses. This study aimed to determine the genomic location of loci that control adult plant resistance (APR) to leaf rust in the pre-Green Revolution landrace accession, Aus27506, from the ‘Watkins Collection’. An Aus27506/Aus27229-derived F7 recombinant inbred (RIL) population was screened under field conditions across three cropping seasons and genotyped with the iSelect 90K Infinium SNP bead chip array. One QTL on each of chromosomes 1BL, 2B and 2DL explained most of the leaf rust response variation in the RIL population and were named QLr.sun-1BL, QLr.sun-2B and QLr.sun-2DL, respectively. QLr.sun-1BL and QLr.sun-2DL were contributed by Aus27506. QLr.sun-1BL is likely Lr46, while QLr.sun-2DL appeared to be a new APR locus. The alternate parent, Aus27229, carried the putatively new APR locus QLr.sun-2B. Comparisons of average severities among RILs carrying these QTL in different combinations indicated that QLr.sun-2B does not interact with either of the other two QTL; however, the combination of QLr.sun-1BL and QLr.sun-2DL reduced disease severity significantly. In-planta fungal quantification assays validated these results. The RILs carrying QLr.sun-1BL and QLr.sun-2DL did not differ significantly from parent Aus27506 in resistance. Aus27506 can be used as a source of adult plant leaf rust resistance in breeding programs.

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