Improving resistance of different apple cultivars using the Rvi6 scab resistance gene in a cisgenic approach based on the Flp/FRT recombinase system

2015 ◽  
Vol 35 (3) ◽  
Author(s):  
Juliane Würdig ◽  
Henryk Flachowsky ◽  
Andrea Saß ◽  
Andreas Peil ◽  
Magda-Viola Hanke
Keyword(s):  
Resistance Gene ◽  
Scab Resistance ◽  
Apple Cultivars

scholarly journals A Molecular Marker Linked to a Scab Resistance Gene (Vf) in Apple

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 619a-619
Author(s):  
Hong Y. Yang ◽  
Schuyler S. Korban ◽  
Jutta Kruger ◽  
Hanna Schmidt
Keyword(s):  
Resistance Gene ◽  
Venturia Inaequalis ◽  
Dominant Gene ◽  
Apple Scab ◽  
Scab Resistance ◽  
Polymorphism Analysis ◽  
Recurrent Parent ◽  
Specific Primers ◽  
Apple Cultivars ◽  
Donor Parent

Apple scab, caused by Venturia inaequalis (Cke.) Wint., is the most serious disease of apple trees. Resistance to V. inaequalis, derived from the small-fruited species Malus floribunda 821, is determined by a major dominant gene Vf. Our major objective is to identify RAPD markers linked to the Vf gene. The approach in this paper is based on the introgression of the Vf gene from M. floribunda into commercial cultivars. Almost 200 random sequence decamer-primers have been used to screen a pair of bulked samples and the donor parent M. floribunda clone 821 for markers linked to the Vf gene conferring resistance to apple scab. A single primer has been identified which generated a PCR fragment, OPK16/1300, from the donor parent M. floribunda clone 821 and the scab-resistant selections/cultivars bulk, but not from the scab-susceptible recurrent parent bulk. Co-segregation analysis using a segregating apple progeny and polymorphism analysis of individual scab-resistant Coop selections/cultivars have confirmed that this marker is linked to the scab-resistance gene Vf. OPK16/1300 has since been cloned and sequenced. Sequence-specific primers of 25 oligonucleotides based on the marker have been synthesized and used to screen further M. floribunda clone 821, scab-susceptible apple cultivars, scab-resistant apple cultivars, and scab-resistant Coop selections. The sequence-specific primers have identified polymorphisms of OPK16/1300 based on the presence or absence of a single band.

scholarly journals A Randomly Amplified Polymorphic DNA (RAPD) Marker Tightly Linked to the Scab-resistance Gene Vf in Apple

1997 ◽  
Vol 122 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Hong Y. Yang ◽  
Schuyler S. Korban ◽  
Jutta Krüger ◽  
Hanna Schmidt
Keyword(s):  
Resistance Gene ◽  
Venturia Inaequalis ◽  
Rapd Marker ◽  
Apple Scab ◽  
Scab Resistance ◽  
Polymorphism Analysis ◽  
Recurrent Parent ◽  
Specific Primers ◽  
Apple Cultivars ◽  
Donor Parent

Almost 200 random sequence decamer primers were used to screen a pair of bulked samples of apple (Malus ×domestica Borkh.) DNA and that of the donor parent Malus floribunda Sieb. clone 821 for molecular markers linked to the Vf gene conferring resistance to apple scab [Venturia inaequalis (Cke.) Wint.]. Identified was a single primer that generated a polymerase chain-reaction (PCR) fragment, OPAR4/1400, from the donor parent M. floribunda clone 821 and the scab-resistant selections/cultivars bulk, but not from the scab-susceptible recurrent-parent bulk. Cosegregation analysis using a segregating apple progeny and polymorphism analysis of individual scab-resistant selections/cultivars confirmed that this marker was linked to the scab-resistance gene Vf OPAR4/1400 was then cloned and sequenced. Sequence-specific primers of 25 oligonucleotides based on the marker were developed and used to screen further M. floribunda clone 821, scab-susceptible apple cultivars, scab-resistant apple cultivars, and scab-resistant Purdue, Rutgers, and Univ. of Illinois apple breeding program selections. The sequence-specific primers identified polymorphisms of OPAR4/1400 based on the presence or absence of a single band. This molecular marker is at a distance of about 3.6 cM from the Vf gene.

scholarly journals A Novel Pear Scab (Venturia nashicola) Resistance Gene, Rvn3, from Interspecific Hybrid Pear (Pyrus pyrifolia × P. communis)

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2632
Author(s):  
Sewon Oh ◽  
Hyeondae Han ◽  
Daeil Kim
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Interspecific Hybrid ◽  
Dominant Gene ◽  
Apple Scab ◽  
Scab Resistance ◽  
Hybrid Population ◽  
Pyrus Pyrifolia ◽  
Pear Scab ◽  
Venturia Nashicola

Asian pear scab is a fungal disease caused by Venturia nashicola. The identification of genes conferring scab resistance could facilitate the breeding of disease-resistant cultivars. Therefore, the present study aimed to identify a scab-resistance gene using an interspecific hybrid population ((Pyrus pyrifolia × P. communis) × P. pyrifolia). Artificial inoculation of V. nashicola was carried out for two years. The segregation ratio (1:1) of resistant to susceptible individuals indicated that resistance to V. nashicola was inherited from P. communis and controlled by a single dominant gene. Based on two years phenotypic data with the Kruskal–Wallis test and interval mapping, 12 common markers were significantly associated with scab resistance. A novel scab resistance gene, Rvn3, was mapped in linkage group 6 of the interspecific hybrid pear, and co-linearity between Rvn3 and one of the apple scab resistance genes, Rvi14, was confirmed. Notably, an insertion in pseudo-chromosome 6 of the interspecific hybrid cultivar showed homology with apple scab resistance genes. Hence, the newly discovered Rvn3 was considered an ortholog of the apple scab resistance gene. Since the mapping population used in the present study is a pseudo-BC1 population, pyramiding of multiple resistance genes to pseudo-BC1 could facilitate the breeding of pear cultivars with durable resistance.

scholarly journals Using scab resistant donors in apple breeding

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 600-603
Author(s):  
B. Gelvonauskis ◽  
D. Gelvonauskienė
Keyword(s):  
Combining Ability ◽  
Apple Scab ◽  
General Combining Ability ◽  
Scab Resistance ◽  
Mean Square ◽  
Genetic Progress ◽  
Apple Cultivars ◽  
The Mean ◽  
Half Diallel ◽  
Selection Of

Apple cultivars were crossed according to the half-diallel and topcross mating design. Apple scab resistance of seedlings was estimated by using a 0–5 scale: 0 – no infection on leaves, 5 – more than 75% of leaf area infected. Percentage of scab resistant seedlings in crosses involving cultivars Prima varied from 35.8% to 67.6%, involving cultivars Florina and Arbat – 51.9–59.5% and 53.5–72.5%, respectively. Significant differences for resistance to scab were found among the crosses. Both general combining ability (GCA) and specific combining ability (SCA) were highly significant. The significance of GCA for scab resistance shows that genetic variability exists for this trait and selection should result in genetic progress, because the mean square for GCA was larger than that calculated for SCA. Therefore, selection of parents on its phenotype should be effective for development of resistant apple cultivars. The significant SCA estimates indicate that a large number of resistant seedlings from particular crosses should be selected.

scholarly journals Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

2004 ◽  
Vol 109 (8) ◽  
pp. 1702-1709 ◽  
Author(s):  
M Gygax ◽  
L Gianfranceschi ◽  
R Liebhard ◽  
M Kellerhals ◽  
C Gessler ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Apple Scab ◽  
Scab Resistance ◽  
Malus Baccata

scholarly journals Quantitative Trait Loci (QTL) Analysis Reveals Both Broad-Spectrum and Isolate-Specific QTL for Scab Resistance in an Apple Progeny Challenged with Eight Isolates of Venturia inaequalis

2004 ◽  
Vol 94 (4) ◽  
pp. 370-379 ◽  
Author(s):  
F. Calenge ◽  
A. Faure ◽  
M. Goerre ◽  
C. Gebhardt ◽  
W. E. Van de Weg ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Venturia Inaequalis ◽  
Scab Resistance ◽  
Breeding Programs ◽  
Apple Breeding ◽  
Trait Loci ◽  
New Races

The major scab resistance gene Vf, extensively used in apple breeding programs, was recently overcome by the new races 6 and 7 of the fungal pathogen Venturia inaequalis. New, more durable, scab resistance genes are needed in apple breeding programs. F1 progeny derived from the cross between partially resistant apple cv. Discovery and apple hybrid ‘TN10-8’ were inoculated in the greenhouse with eight isolates of V. inaequalis, including isolates able to overcome Vf. One major resistance gene, Vg, and seven quantitative trait loci (QTL) were identified for resistance to these isolates. Three QTL on linkage group (LG)12, LG13, and LG15 were clearly isolate-specific. Another QTL on LG5 was detected with two isolates. Three QTL on LG1, LG2, and LG17 were identified with most isolates tested, but not with every isolate. The QTL on LG2 displayed alleles conferring different specificities. This QTL co-localized with the major scab resistance genes Vr and Vh8, whereas the QTL on LG1 colocalized with Vf. These results contribute to a better understanding of the genetic basis of the V. inaequalis-Malus × domestica interaction.

scholarly journals Identification of functional apple scab resistance gene promoters

2005 ◽  
Vol 110 (6) ◽  
pp. 1119-1126 ◽  
Author(s):  
E. Silfverberg-Dilworth ◽  
S. Besse ◽  
R. Paris ◽  
E. Belfanti ◽  
S. Tartarini ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Apple Scab ◽  
Scab Resistance ◽  
Gene Promoters

scholarly journals Identification and mapping of the novel apple scab resistance gene Vd3

2009 ◽  
Vol 5 (3) ◽  
pp. 475-482 ◽  
Author(s):  
J.M. Soriano ◽  
S.G. Joshi ◽  
M. van Kaauwen ◽  
Y. Noordijk ◽  
R. Groenwold ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Apple Scab ◽  
Scab Resistance ◽  
The Novel

scholarly journals Apple Contains Receptor-like Genes Homologous to the Cladosporium fulvum Resistance Gene Family of Tomato with a Cluster of Genes Cosegregating with Vf Apple Scab Resistance

2001 ◽  
Vol 14 (4) ◽  
pp. 508-515 ◽  
Author(s):  
Boris A. Vinatzer ◽  
Andrea Patocchi ◽  
Luca Gianfranceschi ◽  
Stefano Tartarini ◽  
Hong-Bin Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Transmembrane Domain ◽  
Venturia Inaequalis ◽  
Scab Resistance ◽  
Common Disease ◽  
Resistance Locus ◽  
Cladosporium Fulvum ◽  
High Quality

Scab caused by the fungal pathogen Venturia inaequalis is the most common disease of cultivated apple (Malus × domestica Borkh.). Monogenic resistance against scab is found in some small-fruited wild Malus species and has been used in apple breeding for scab resistance. Vf resistance of Malus floribunda 821 is the most widely used scab resistance source. Because breeding a high-quality cultivar in perennial fruit trees takes dozens of years, cloning disease resistance genes and using them in the transformation of high-quality apple varieties would be advantageous. We report the identification of a cluster of receptor-like genes with homology to the Cladosporium fulvum (Cf) resistance gene family of tomato on bacterial artificial chromosome clones derived from the Vf scab resistance locus. Three members of the cluster were sequenced completely. Similar to the Cf gene family of tomato, the deduced amino acid sequences coded by these genes contain an extracellular leucine-rich repeat domain and a transmembrane domain. The transcription of three members of the cluster was determined by reverse transcription-polymerase chain reaction to be constitutive, and the transcription and translation start of one member was verified by 5′ rapid amplification of cDNA ends. We discuss the parallels between Cf resistance of tomato and Vf resistance of apple and the possibility that one of the members of the gene cluster is the Vf gene. Cf homologs from other regions of the apple genome also were identified and are likely to present other scab resistance genes.

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