Identification and fine mapping of qPBR10-1, a novel locus controlling panicle blast resistance in Pigm-containing P/TGMS line

Rice blast is a global threat to food security with up to 50% yield losses. Panicle blast is a more severe form of rice blast and the response of rice plant to leaf and panicle blast is distinct in different genotypes. To understand the specific response of rice in panicle blast, transcriptome analysis of blast resistant cultivar Tetep, and susceptible cultivar HP2216 was carried out using RNA-Seq approach after 48, 72 and 96 h of infection with Magnaporthe oryzae along with mock inoculation. Transcriptome data analysis of infected panicle tissues revealed that 3553 genes differentially expressed in HP2216 and 2491 genes in Tetep, which must be the responsible factor behind the differential disease response. The defense responsive genes are involved mainly in defense pathways namely, hormonal regulation, synthesis of reactive oxygen species, secondary metabolites and cell wall modification. The common differentially expressed genes in both the cultivars were defense responsive transcription factors, NBS-LRR genes, kinases, pathogenesis related genes and peroxidases. In Tetep, cell wall strengthening pathway represented by PMR5, dirigent, tubulin, cell wall proteins, chitinases, and proteases was found to be specifically enriched. Additionally, many novel genes having DOMON, VWF, and PCaP1 domains which are specific to cell membrane were highly expressed only in Tetep post infection, suggesting their role in panicle blast resistance. Thus, our study shows that panicle blast resistance is a complex phenomenon contributed by early defense response through ROS production and detoxification, MAPK and LRR signaling, accumulation of antimicrobial compounds and secondary metabolites, and cell wall strengthening to prevent the entry and spread of the fungi. The present investigation provided valuable candidate genes that can unravel the mechanisms of panicle blast resistance and help in the rice blast breeding program.

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Resistance spectrum assay and fine mapping of the blast resistance gene from a rice experimental line, IRBLta2-Re

Euphytica ◽

10.1007/s10681-013-0992-1 ◽

2013 ◽

Vol 195 (2) ◽

pp. 209-216 ◽

Cited By ~ 5

Author(s):

Shen Chen ◽

Jing Su ◽

Jingluan Han ◽

Wenjuan Wang ◽

Congying Wang ◽

...

Keyword(s):

Resistance Gene ◽

Fine Mapping ◽

Blast Resistance ◽

Blast Resistance Gene ◽

Experimental Line ◽

Resistance Spectrum

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The OsOXO2, OsOXO3 and OsOXO4 Positively Regulate Panicle Blast Resistance in Rice

Rice ◽

10.1186/s12284-021-00494-9 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Jingfang Dong ◽

Lian Zhou ◽

Aiqing Feng ◽

Shaohong Zhang ◽

Hua Fu ◽

...

Keyword(s):

Signaling Pathways ◽

Signaling Pathway ◽

Molecular Mechanisms ◽

Blast Resistance ◽

Aba Signaling ◽

Leaf Blast ◽

Expression Levels ◽

Panicle Blast ◽

Localization Analysis ◽

Sa Signaling

Abstract Background Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance are still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown. Results In the present study, we have confirmed that the three Oxalate oxidase (OXO) genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO proteins are all localized in the nucleus and cytoplasm. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance. More H2O2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the silencing plants exhibited less H2O2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the phytohormone signaling related gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways but suppression of SA signaling pathway. Conclusion OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H2O2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance could be regulated by ABA, SA and JA, and may be associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.

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Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication

The Plant Journal ◽

10.1111/j.1365-313x.2010.04348.x ◽

2010 ◽

Vol 64 (3) ◽

pp. 498-510 ◽

Cited By ~ 149

Author(s):

Nagao Hayashi ◽

Haruhiko Inoue ◽

Takahiro Kato ◽

Taketo Funao ◽

Masaki Shirota ◽

...

Keyword(s):

Resistance Gene ◽

Genome Duplication ◽

Blast Resistance ◽

Blast Resistance Gene ◽

Panicle Blast ◽

Lrr Protein

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Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates

Molecular Breeding ◽

10.1007/s11032-008-9175-5 ◽

2008 ◽

Vol 22 (2) ◽

pp. 289-299 ◽

Cited By ~ 26

Author(s):

Xin Xu ◽

N. Hayashi ◽

C. T. Wang ◽

H. Kato ◽

T. Fujimura ◽

...

Keyword(s):

Resistance Gene ◽

Fine Mapping ◽

Rice Blast ◽

Blast Resistance ◽

Rice Blast Resistance ◽

Blast Resistance Gene

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Fine Mapping of a New Race-Specific Blast Resistance Gene, Pi-hk2, in Japonica Heikezijing from Taihu Region of China

Phytopathology ◽

10.1094/phyto-03-16-0151-r ◽

2017 ◽

Vol 107 (1) ◽

pp. 84-91 ◽

Cited By ~ 2

Author(s):

Wanwan He ◽

Nengyan Fang ◽

Ruisen Wang ◽

Yunyu Wu ◽

Guoying Zeng ◽

...

Keyword(s):

Resistance Gene ◽

Fine Mapping ◽

Broad Spectrum ◽

Blast Resistance ◽

Recombinant Inbred Lines ◽

Japonica Rice ◽

Phenotypic Variance ◽

Chromosome 11 ◽

Essential Information ◽

Broad Spectrum Resistance

Heikezijing, a japonica rice landrace from the Taihu region of China, exhibited broad-spectrum resistance to more than 300 isolates of the blast pathogen (Magnaporthe oryzae). In our previous research, we fine mapped a broad-spectrum resistance gene, Pi-hk1, in chromosome 11. In this research, 2010-9(G1), one of the predominant races of blast in the Taihu Lake region of China, was inoculated into 162 recombinant inbred lines (RIL) and two parents, Heikezijing and Suyunuo, for mapping the resistance-blast quantitative trait loci (QTL). Three QTL (Lsqtl4-1, Lsqtl9-1, and Lsqtl11-1) associated with lesion scores were detected on chromosomes 4, 9, and 11 and two QTL (Lnqtl1-1 and Lnqtl9-1) associated with average lesion numbers were detected on chromosomes 1 and 9. The QTL Lsqtl9-1 conferring race-specific resistance to 2010-9(G1) at seedling stages showed logarithm of the odds scores of 9.10 and phenotypic variance of 46.19% and might be a major QTL, named Pi-hk2. The line RIL84 with Pi-hk2 derived from a cross between Heikezijing and Suyunuo was selected as Pi-hk2 gene donor for developing fine mapping populations. According to the resistance evaluation of recombinants of three generations (BC1F2, BC1F3, and BC1F4), Pi-hk2 was finally mapped to a 143-kb region between ILP-19 and RM24048, and 18 candidate genes were predicted, including genes that encode pleiotropic drug resistance protein 4 (n = 2), WRKY74 (n = 1), cytochrome b5-like heme/steroid-binding domain containing protein (n = 1), protein kinase (n = 1), and ankyrin repeat family protein (n = 1). These results provide essential information for cloning of Pi-hk2 and its potential utility in breeding resistant rice cultivars by marker-assisted selection.

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