Selection of housekeeping genes for gene expression studies on the development of fruit bearing shoots in Chinese jujube (Ziziphus jujube Mill.)

2008 ◽  
Vol 36 (8) ◽  
pp. 2183-2190 ◽  
Author(s):  
Hai-feng Sun ◽  
Yu-ping Meng ◽  
Gui-mei Cui ◽  
Qiu-fen Cao ◽  
Jie Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Housekeeping Genes ◽  
Chinese Jujube ◽  
Expression Studies ◽  
Ziziphus Jujube ◽  
Gene Expression Studies ◽  
Selection Of

Selection of Housekeeping Genes for Gene Expression Studies in a Rat Model of Irinotecan-Induced Mucositis

Chemotherapy ◽  
2011 ◽  
Vol 57 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Noor Al-Dasooqi ◽  
Joanne M. Bowen ◽  
Rachel J. Gibson ◽  
Richard M. Logan ◽  
Andrea M. Stringer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Model ◽  
Housekeeping Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR

2008 ◽  
Vol 9 (1) ◽  
pp. 28 ◽  
Author(s):  
Carlos Infante ◽  
Makoto P Matsuoka ◽  
Esther Asensio ◽  
José Cañavate ◽  
Michael Reith ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Housekeeping Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states

2008 ◽  
Vol 9 (1) ◽  
pp. 64 ◽  
Author(s):  
Nicholas Silver ◽  
Emanuele Cotroneo ◽  
Gordon Proctor ◽  
Samira Osailan ◽  
Katherine L Paterson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Submandibular Gland ◽  
Housekeeping Genes ◽  
Adult Rat ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Rat Submandibular Gland ◽  
Selection Of

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

Selection of reference genes for gene expression studies in chronically hypoxic heart

2018 ◽  
Vol 120 ◽  
pp. 22-23
Author(s):  
D. Benak ◽  
D. Sotakova ◽  
J. Neckar ◽  
F. Kolar ◽  
M. Hlavackova
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Hypoxic Heart ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dorota M. Krzyżanowska ◽  
Anna Supernat ◽  
Tomasz Maciąg ◽  
Marta Matuszewska ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Quorum Sensing ◽  
Reference Genes ◽  
Significance Threshold ◽  
Expression Studies ◽  
Reverse Transcription Quantitative Pcr ◽  
Gene Expression Studies ◽  
The Stability ◽  
Selection Of

Abstract Reverse transcription quantitative PCR (RT-qPCR), a method of choice for quantification of gene expression changes, requires stably expressed reference genes for normalization of data. So far, no reference genes were established for the Alphaproteobacteria of the genus Ochrobactrum. Here, we determined reference genes for gene expression studies in O. quorumnocens A44. Strain A44 was cultured under 10 different conditions and the stability of expression of 11 candidate genes was evaluated using geNorm, NormFinder and BestKeeper. Most stably expressed genes were found to be rho, gyrB and rpoD. Our results can facilitate the choice of reference genes in the related Ochrobactrum strains. O. quorumnocens A44 is able to inactivate a broad spectrum of N-acyl homoserine lactones (AHLs) – the quorum sensing molecules of many Gram-negative bacteria. This activity is attributed to AiiO hydrolase, yet it remains unclear whether AHLs are the primary substrate of this enzyme. Using the established RT-qPCR setup, we found that the expression of the aiiO gene upon exposure to two AHLs, C6-HLS and 3OC12-HSL, does not change above the 1-fold significance threshold. The implications of this finding are discussed in the light of the role of quorum sensing-interfering enzymes in the host strains.

scholarly journals Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (Globodera spp.)

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193840 ◽  
Author(s):  
Michael Sabeh ◽  
Marc-Olivier Duceppe ◽  
Marc St-Arnaud ◽  
Benjamin Mimee
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Potato Cyst Nematodes ◽  
Cyst Nematodes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

Validation of housekeeping genes as an internal control for gene expression studies in the brain of ovariectomized rats treated with tibolone

Gene ◽  
2021 ◽  
Vol 769 ◽  
pp. 145255
Author(s):  
Iris A. Feria-Romero ◽  
Iván Bribiesca-Cruz ◽  
Angélica Coyoy-Salgado ◽  
Julia J. Segura-Uribe ◽  
Guadalupe Bautista-Poblet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Internal Control ◽  
Housekeeping Genes ◽  
Ovariectomized Rats ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
The Brain

scholarly journals Selection of Reference Genes for Gene Expression Studies related to lung injury in a preterm lamb model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Prue M. Pereira-Fantini ◽  
Anushi E. Rajapaksa ◽  
Regina Oakley ◽  
David G. Tingay
Keyword(s):  
Gene Expression ◽  
Lung Injury ◽  
Reference Genes ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document