Development and application of a loop-mediated isothermal amplification method on rapid detection Escherichia coli O157 strains from food samples

2009 ◽  
Vol 37 (5) ◽  
pp. 2183-2188 ◽  
Author(s):  
Xihong Zhao ◽  
Yanmei Li ◽  
Li Wang ◽  
Lijun You ◽  
Zhenbo Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Food Samples ◽  
Isothermal Amplification ◽  
Escherichia Coli O157 ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method

Rapid Detection of Viable Escherichia coli O157 by Coupling Propidium Monoazide with Loop-Mediated Isothermal Amplification

2013 ◽  
Vol 23 (12) ◽  
pp. 1708-1716 ◽  
Author(s):  
Xihong Zhao ◽  
Jun Wang ◽  
Fereidoun Forghani ◽  
Joong-Hyun Park ◽  
Myoung-Su Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Escherichia Coli O157 ◽  
Propidium Monoazide ◽  
Loop Mediated Isothermal Amplification

Detection of Enteroaggregative Escherichia coli by Loop-Mediated Isothermal Amplification

2010 ◽  
Vol 73 (6) ◽  
pp. 1064-1072 ◽  
Author(s):  
EIJI YOKOYAMA ◽  
MASAKO UCHIMURA ◽  
KENITIRO ITO
Keyword(s):  
Escherichia Coli ◽  
Food Samples ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Bacterial Strains ◽  
Exact Test ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Contaminated Food ◽  
Better Than

A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia coli (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than that of PCR in a study of serially diluted EAEC cells. The LAMP method was significantly more effective than was PCR in detecting EAEC-contaminated food samples (Fisher's exact test, P < 0.05). Therefore, the LAMP method described here should be useful for detecting small numbers of EAEC cells in food samples.

scholarly journals Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method

2004 ◽  
Vol 70 (1) ◽  
pp. 621-624 ◽  
Author(s):  
Ram Savan ◽  
Arisa Igarashi ◽  
Satoru Matsuoka ◽  
Masahiro Sakai
Keyword(s):  
Rapid Detection ◽  
Japanese Flounder ◽  
Isothermal Amplification ◽  
Edwardsiella Tarda ◽  
Fish Disease ◽  
Fish Pathogen ◽  
Loop Mediated Isothermal Amplification ◽  
Diagnostic Protocol ◽  
Amplification Method ◽  
Hemolysin Gene

ABSTRACT Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65°C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infected Japanese flounder kidney, and spleen and seawater cultures was possible. We have developed a rapid and sensitive diagnostic protocol for edwardsiellosis detection in fish. This is the first report of the application of LAMP for the diagnosis of a fish pathogen.

scholarly journals Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification

10.19082/2576 ◽  
2016 ◽  
Vol 8 (6) ◽  
pp. 2576-2585 ◽  
Author(s):  
Reza Ranjbar ◽  
Maryam Erfanmanesh ◽  
Davoud Afshar ◽  
Mohsen Mohammadi ◽  
Omar Ghaderi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Visual Detection ◽  
Enterohemorrhagic Escherichia Coli ◽  
Isothermal Amplification ◽  
Escherichia Coli O157 ◽  
Loop Mediated Isothermal Amplification
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