Rapid Detection of Escherichia coli O157:H7 by Loop-Mediated Isothermal Amplification Coupled with a Lateral Flow Assay Targeting the z3276 Genetic Marker

2021 ◽  
Author(s):  
Xuefeng Xia ◽  
Bicheng Zhang ◽  
Jing Wang ◽  
Bin Li ◽  
Kongwang He ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Marker ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Lateral Flow Assay ◽  
Escherichia Coli O157 ◽  
Loop Mediated Isothermal Amplification

Rapid Detection of Viable Escherichia coli O157 by Coupling Propidium Monoazide with Loop-Mediated Isothermal Amplification

2013 ◽  
Vol 23 (12) ◽  
pp. 1708-1716 ◽  
Author(s):  
Xihong Zhao ◽  
Jun Wang ◽  
Fereidoun Forghani ◽  
Joong-Hyun Park ◽  
Myoung-Su Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Escherichia Coli O157 ◽  
Propidium Monoazide ◽  
Loop Mediated Isothermal Amplification

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

scholarly journals Visual Detection of Enterohemorrhagic Escherichia coli O157:H7 Using Loop-Mediated Isothermal Amplification

10.19082/2576 ◽  
2016 ◽  
Vol 8 (6) ◽  
pp. 2576-2585 ◽  
Author(s):  
Reza Ranjbar ◽  
Maryam Erfanmanesh ◽  
Davoud Afshar ◽  
Mohsen Mohammadi ◽  
Omar Ghaderi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Visual Detection ◽  
Enterohemorrhagic Escherichia Coli ◽  
Isothermal Amplification ◽  
Escherichia Coli O157 ◽  
Loop Mediated Isothermal Amplification
Sign in / Sign up

Export Citation Format

Share Document