Demethylase FTO promotes mechanical stress induced osteogenic differentiation of BMSCs with up-regulation of HIF-1α

2022 ◽  
Author(s):  
Renhao Sun ◽  
Chunxi Zhang ◽  
Yicong Liu ◽  
Zhenggang Chen ◽  
Wen Liu ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Mechanical Stress

scholarly journals Induction of Osteogenic Differentiation of Adipose Derived Stem Cells by Microstructured Nitinol Actuator-Mediated Mechanical Stress

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51264 ◽  
Author(s):  
Sarah Strauß ◽  
Sonja Dudziak ◽  
Ronny Hagemann ◽  
Stephan Barcikowski ◽  
Malte Fliess ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Adipose Derived Stem Cells

scholarly journals Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through GCN5 and Wnt/β-Catenin

2021 ◽  
Vol 9 ◽  
Author(s):  
Yanchang Liu ◽  
Wendan Cheng ◽  
Yao Zhao ◽  
Liang Gao ◽  
Yongyun Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Critical Role ◽  
Mechanical Strain ◽  
Biological Behavior ◽  
Mechanical Stimuli ◽  
Mechanical Signals ◽  
Alkaline Phosphatase Staining

Bone marrow mesenchymal stem cells (BMSCs) play a critical role in bone formation and are extremely sensitive to external mechanical stimuli. Mechanical signals can regulate the biological behavior of cells on the surface of titanium-related prostheses and inducing osteogenic differentiation of BMSCs, which provides the integration of host bone and prosthesis benefits. But the mechanism is still unclear. In this study, BMSCs planted on the surface of TiO2 nanotubes were subjected to cyclic mechanical stress, and the related mechanisms were explored. The results of alkaline phosphatase staining, real-time PCR, and Western blot showed that cyclic mechanical stress can regulate the expression level of osteogenic differentiation markers in BMSCs on the surface of TiO2 nanotubes through Wnt/β-catenin. As an important member of the histone acetyltransferase family, GCN5 exerted regulatory effects on receiving mechanical signals. The results of the ChIP assay indicated that GCN5 could activate the Wnt promoter region. Hence, we concluded that the osteogenic differentiation ability of BMSCs on the surface of TiO2 nanotubes was enhanced under the stimulation of cyclic mechanical stress, and GCN5 mediated this process through Wnt/β-catenin.

scholarly journals Inflammation and Mechanical Stress Stimulate Osteogenic Differentiation of Human Aortic Valve Interstitial Cells

2018 ◽  
Vol 9 ◽  
Author(s):  
Maria Bogdanova ◽  
Aleksandra Kostina ◽  
Katarina Zihlavnikova Enayati ◽  
Arsenii Zabirnyk ◽  
Anna Malashicheva ◽  
...  
Keyword(s):  
Aortic Valve ◽  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Interstitial Cells ◽  
Valve Interstitial Cells ◽  
Human Aortic Valve

scholarly journals Mechanical stress affects methylation pattern of GNAS isoforms and osteogenic differentiation of hAT-MSCs

2017 ◽  
Vol 1864 (8) ◽  
pp. 1371-1381 ◽  
Author(s):  
Angeliki-Maria Vlaikou ◽  
Dimitrios Kouroupis ◽  
Argyro Sgourou ◽  
Georgios S. Markopoulos ◽  
Eleni Bagli ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Methylation Pattern

scholarly journals Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction

2022 ◽  
Vol 9 ◽  
Author(s):  
Xun Xi ◽  
Zixuan Li ◽  
Hong Liu ◽  
Shuai Chen ◽  
Dongxu Liu
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Protein Interaction ◽  
Periodontal Ligament ◽  
Tandem Mass ◽  
Periodontal Ligament Stem Cells ◽  
Expression Levels ◽  
Protein Protein Interaction ◽  
Nrf2 Activation

Nuclear factor erythroid-2-related factor-2 (Nrf2), the major transcriptional regulator in antioxidant response and cellular defense, had the vital effect on regulating osteogenic differentiation. Our previous study revealed that Nrf2 activation was involved in cyclic mechanical stress-stimulated osteogenic differentiation in the human periodontal ligament stem cells (PDLSCs). However, the mechanisms of Nrf2 underlying this process remained unclear. The goal of the study was to explore the mechanisms of Nrf2 in PDLSCs during cyclic mechanical stress-stimulated osteogenic differentiation via the tandem mass tag (TMT)-based liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis. And we applied tert-Butylhydroquinone (t-BHQ), the Nrf2 activator, to the orthodontic rats and detected the expression levels of the osteogenesis markers by immunohistochemistry (IHC) staining. Our results showed that Nrf2 activation in PDLSCs was involved in cyclic mechanical stress-stimulated osteogenic differentiation via phosphoinositide 3 kinase (PI3K)/protein kinase B (Akt) pathway. The protein-protein interaction between Akt and Nrf2 was detected. And the protein-protein interaction between heme oxygenase 1 (HO1) and superoxide dismutase 2 (SOD2), the downstream antioxidants of Nrf2, was associated with cyclic mechanical stress-stimulated osteogenic differentiation. T-BHQ enhanced the expression levels of the osteogenesis markers in orthodontic rats. Nrf2 might possess the potential to be a feasible molecular target in orthodontics.

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