Determination of the kinetic parameters of glycerol diffusion in the gingival and dentinal tissue of a human tooth using optical method: in vitro studies

2020 ◽  
Vol 52 (2) ◽  
Author(s):  
A. A. Selifonov ◽  
V. V. Tuchin
2019 ◽  
Vol 126 (6) ◽  
pp. 832
Author(s):  
А.А. Селифонов ◽  
О.Г. Шаповал ◽  
А.Н. Микеров ◽  
В.В. Тучин

The work is devoted to the determination of the diffusion coefficients of methylene blue in pure aqueous solution and in a micellar solution of a cationic surfactant in human tooth dentinal sections in vitro using diffuse reflectance optical spectroscopy and the free diffusion model. The determination of the diffusion coefficient of methylene blue for an aqueous solution is (6.74 ± 1.32) • 10−6 cm2/s and (1.93 ± 0.24) • 10−6 cm2/s for methylene blue in micellar solution. Studies of toxicity in daylight in the absence of laser radiation of methylene blue solutions in water and in solution of cetylpyridinium chloride, as well as the photodynamic effect of laser radiation (662 nm) on cells of Candida albicans, Staphylococcus aureus FDA 209P and Lactobacillus were carried out. The effect of laser radiation has a pronounced suppressive effect on all the studied microbial strains.


1990 ◽  
Vol 36 (9) ◽  
pp. 1638-1641 ◽  
Author(s):  
S C Kazmierczak ◽  
W J Castellani ◽  
F Van Lente ◽  
E D Hodges ◽  
B Udis

Abstract We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenzyme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.


2011 ◽  
Vol 36 (3) ◽  
pp. 159-166 ◽  
Author(s):  
Leandro Augusto Calixto ◽  
Anderson Rodrigo Moraes de Oliveira ◽  
Valquíria Aparecida Polisel Jabor ◽  
Pierina Sueli Bonato

2016 ◽  
Vol 32 (11) ◽  
pp. 1385-1393 ◽  
Author(s):  
F.Z.M. Soares ◽  
A. Follak ◽  
L.S. da Rosa ◽  
A.F. Montagner ◽  
T.L. Lenzi ◽  
...  

1981 ◽  
Vol 15 ◽  
pp. 515-515 ◽  
Author(s):  
A Vargas ◽  
E O Reiter ◽  
K Kula ◽  
L J Rodriguez-Rigau ◽  
E Steinberger ◽  
...  

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