Development and Validation of the UV Spectrophotometric Method for Simultaneous Determination of Paracetamol and Pseudoephedrine in Bulk and Combined Tablet Dosage Form

2021 ◽  
Vol 54 (12) ◽  
pp. 1306-1310
Author(s):  
Mohamed S. Ali ◽  
Tilal Elsaman
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage

scholarly journals Development DEVELOPMENT AND VALIDATION OF NOVEL ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF DALFAMPRIDINE IN BULK AND IN PHARMACEUTICAL FORMULATION

2019 ◽  
pp. 275-279
Author(s):  
VAIBHAV S KHODKE ◽  
GAME MD
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Quality Criteria ◽  
Pharmaceutical Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Good Agreement

Objective: The objective of the present study is to develop ultraviolet (UV)-spectroscopic method using pure drug and tablet dosage form that consistently produces a drug with a minimal variation that adheres to quality criteria of purity, identity, and potency. Methods: UV-spectrophotometric method has been developed using a solvent composed of methanol:water (30:70) as a diluent to determine the dalfampridine (DFP) content in bulk and pharmaceutical dosage form at predetermined λmax of 262 nm. Results: It was proved linear in the range of 02–12 μg/ml and exhibited a good correlation coefficient (r2 = 0.9915) and excellent mean recovery (0.004136347%). This method was successfully applied to the determination of DFP content of marketed tablet Dalstep 10 mg (Sun Pharmaceutical Pvt. Ltd.,) from India; the results were in good agreement with the label claims. Conclusion: The method proved to be simple, accurate, precise, specific, robust, and less time consuming and can be applied for the determination of DFP in bulk and marketed formulation.

Analytical Method Development and Validation for Telmisartan, Chlorthalidone and Amlodipine by UV- Spectroscopic Method.

2021 ◽  
pp. 6049-6054
Author(s):  
Rutuja M. Sanap ◽  
Sarika R. Wavhale ◽  
Vaibhavi V. Kunjir ◽  
Rajkumar V. Shete
Keyword(s):  
Simultaneous Determination ◽  
Analytical Method ◽  
Dosage Form ◽  
Method Development ◽  
Correction Method ◽  
Absorption Correction ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

A simple, sensitive and accurate UV- spectrophotometric absorption correction method has been developed for simultaneous determination of Telmisartan, Amlodipine and Chlorthalidone in combined tablet dosage form. Analytical method development and validation plays important role in the discovery manufacture of pharmaceuticals and development. In this paper, absorption correction method is used for multi-component analysis. The wavelengths selected for the analysis were 311nm for Telmisartan, 228nm for Chlorthalidone and 253nm for Amlodipine. Beer’s law obeyed the concentration range of 2-10 µg /ml, 2-10 µg /ml and 5-25 µg/ ml for Telmisartan, Amlodipine and Chlorthalidone. Methanol is used as a solvent. The accuracy of the method was assessed by recovery studies and was found between the range of 100% to 110% for Telmisartan, 85% to 110% for Amlodipine and 85% to 105% Chlorthalidone. The % RSD value was found to be less than 2. Thus, the method was simple, precise, economic, rapid, accurate and can be successfully applied for simultaneous determination of Telmisartan, Amlodipine and Chlorthalidone in combined tablet dosage form.

scholarly journals Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe

2010 ◽  
Vol 7 (s1) ◽  
pp. S197-S202 ◽  
Author(s):  
Effat Souri ◽  
Masoud Amanlou
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Mechanisms Of Action ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Form ◽  
Zero Crossing ◽  
First Order ◽  
Development And Validation

A combination of simvastatin and ezetimibe with complementary mechanisms of action is used for treating high levels of cholesterol in the blood. The aim of this study was to develop a rapid and sensitive derivative spectrophotometric method for analysis of these drugs in combined dosage forms. A first order derivative spectrophotometric method was developed for simultaneous determination of simvastatin and ezetimibe using zero-crossing technique. The measurements were carried out at 219 and 265 nm for simvastatin and ezetimibe respectively. The described method was found to be linear (r2>0.999) over the range of 2-40 μg/mL for simvastatin in the presence of 10 μg/mL ezetimibe at 219 nm and in the range of 1-20 μg/mL of ezetimibe in the presence of 20 μg/mL of simvastatin at 265 nm. The within-day and between-day precision values for both drugs were less than 3% (CV). Also, good recoveries were obtained with both synthetic mixtures and commercial tablets. The proposed method was successfully applied for simultaneous determination of simvastatin and ezetimibe in a pharmaceutical dosage form without any interference from excipients.

DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF FAMOTIDINE, DICLOFENAC AND PARACETAMOL IN THEIR COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (09) ◽  
pp. 60-64
Author(s):  
P. P Desai ◽  
◽  
N. R. Patel ◽  
H. G Bhatt
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Absorbance Measurement ◽  
Validation Parameters ◽  
Stock Solutions ◽  
Development And Validation ◽  
Tablet Dosage

Famotidine (FAM), Diclofenac (DCF) and Paracetamol (PCM) are used in combination for musculoskeletal disorders. A simple, sensitive, rapid, precise, reproducible and accurate spectrophotometric method for simultaneous determination of FAM, DCF and PCM was developed. The method was based on UV spectrophotometric determination of three drugs using simultaneous equation method. The stock solutions were prepared in methanol AR. Absorbance measurement was carried out at 288.4 nm, 281.2nm and 248.2 nm for FAM, DCF and PCM respectively. Beer Lambert law was obeyed in the concentration range of 1-30μg/mL for FAM, 2-40μg/mL for DCF and 1-20μg/mL for PCM. The results of the analysis were tested and validated for various validation parameters statistically and by recovery studies according to the International Conference on Harmonization Q2B guidelines. The utility of the developed method has been demonstrated by analysis of commercially available tablet dosage form.

UV Spectrophotometric Method Development and Validation of Darunavir in bulk and Solid Dosage Form

2021 ◽  
pp. 3262-3264
Author(s):  
Varsha Tegeli ◽  
Avinash Birajdar ◽  
Vinod Matole
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Uv Spectrum ◽  
Solid Dosage ◽  
Highly Sensitive ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Tablet Dosage

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Darunavir in Tablet dosage form with¬¬¬ 0.1N HCl. Method: The method is based on the formation of a colorless complex. The UV spectrum of Darunavir in 0.1N HCl showed maximum wavelength at 298nm. Beer’s law is valid in the concentration range of 10-60µg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-60µg/ml with the regression equation y=0.0113x+0.0098, and regression coefficient i.e. r2=0.9992 moreover, the method was found to be highly sensitive with LOD (1.85µg/ml) and LOQ (5.62µg/ml). Conclusion: Based on the results the proposed method can be successfully applied for the assay of Darunavir in various tablet dosage forms.

Sign in / Sign up

Export Citation Format

Share Document