Male Gametic Cell-specific Histone gH2A Gene of Lilium longiflorum: Genomic Structure and Promoter Activity in the Generative Cell

The genomic structure and transcriptional regulation of the rat apelin receptor (APJR) were analysed by rapid amplification of 5′ cDNA ends (5′-RACE), transient expression assays and DNA–protein interaction. Analysis of the 5′-flanking region of a rat genomic clone shows no TATA box, but a putative CAAT box and several putative binding sites for transcription factors are present. Two transcriptional start sites were identified by 5′-RACE, RNase protection and primer extension analyses. Promoter activity was exhibited in the APJR- expressing SH-SY5Y cell line as well as in COS-7 and Chinese hamster ovary (CHO-K1) cells. Consecutive 5′-deletion analysis revealed the highest promoter activity in a region between bp −966 and −165. DNaseI footprint analysis revealed seven protected regions and electrophoretic mobility shift, super-shift and competition assays identified individual DNA–protein complexes capable of binding Sp1, estrogen receptor (ER)α, glucocorticoid receptor and CCAAT enhancer binding protein (C/EBP)γ transcription factors. Site-directed mutagenesis identified an individual Sp1 motif that plays a major role in activation of the APJR promoter and also demonstrated constitutive transcriptional regulation of the promoter by estrogen and glucocorticoid receptors. Promoter regulation by the cAMP-dependent signal cascade was also shown.

Download Full-text

Comparative study of four interleukin 17 cytokines of tongue sole Cynoglossus semilaevis : Genomic structure, expression pattern, and promoter activity

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2015.09.020 ◽

2015 ◽

Vol 47 (1) ◽

pp. 321-330 ◽

Cited By ~ 12

Author(s):

Heng Chi ◽

Li Sun

Keyword(s):

Comparative Study ◽

Expression Pattern ◽

Promoter Activity ◽

Genomic Structure ◽

Cynoglossus Semilaevis ◽

Interleukin 17 ◽

Tongue Sole

Download Full-text

Genomic structure and promoter characterization of the human Sprouty4 gene, a novel regulator of lung morphogenesis

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.00430.2003 ◽

2004 ◽

Vol 287 (1) ◽

pp. L52-L59 ◽

Cited By ~ 13

Author(s):

Wei Ding ◽

Saverio Bellusci ◽

Wei Shi ◽

David Warburton

Keyword(s):

Promoter Activity ◽

Molecular Mechanisms ◽

Core Promoter ◽

Genomic Structure ◽

Inducible Promoter ◽

Luciferase Reporter ◽

Luciferase Reporter Gene ◽

Significant Similarity ◽

The Core ◽

Flanking Region

The expression of Sprouty4 ( Spry4), an intracellular FGF receptor antagonist, shows a temporally and spatially restricted pattern in embryonic lung and is induced by ERK signaling. To clarify the molecular mechanisms regulating Spry4 transcription, the genomic structure of the human Sprouty4 ( hSpry4) gene was first determined by using the GenomeWalker kit. The hSpry4 gene spans > 14 kb and is organized in three exons and two introns. Multiple transcription start sites were subsequently mapped by 5′-rapid amplification of cDNA ends. Analysis of up to 4 kb of sequence in the 5′-flanking region of the gene showed the presence of multiple potential transcription factor binding sites but no TATA or CAAT boxes. Transient transfection using luciferase reporter gene constructs with progressive deletions of the hSpry4 5′-flanking region revealed that the core promoter activity is located within the proximal 0.4-kb region, whereas the minimal ERK-inducible promoter activity is between −69 and −31. Homology analysis further showed that the core promoter region of the hSpry4 gene exhibits significant similarity to the 5′-flanking region of the mouse gene.

Download Full-text