Effects of macroelements and nitrogen source on biomass accumulation and withanolide-A production from cell suspension cultures of Withania somnifera (L.) Dunal

2010 ◽  
Vol 104 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Praveen Nagella ◽  
Hosakatte Niranjana Murthy
Keyword(s):  
Cell Suspension ◽  
Nitrogen Source ◽  
Withania Somnifera ◽  
Cell Suspension Cultures ◽  
Biomass Accumulation ◽  
Suspension Cultures ◽  
Withanolide A

In vitro Withanolide Production by Withania somnifera L. Cultures

2008 ◽  
Vol 63 (5-6) ◽  
pp. 409-412 ◽  
Author(s):  
Farzana Sabir ◽  
Neelam S. Sangwan ◽  
Narayan D. Chaurasiya ◽  
Laxmi N. Misra ◽  
Rajender S. Sangwan
Keyword(s):  
Cell Suspension ◽  
Withania Somnifera ◽  
Cell Suspension Cultures ◽  
Multiple Shoots ◽  
Suspension Cultures ◽  
Root Cultures ◽  
Withanolide A ◽  
Root Callus ◽  
Pharmacologically Active

In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at lower levels.

Innate endophytic fungus, Aspergillus terreus as biotic elicitor of withanolide A in root cell suspension cultures of Withania somnifera

2019 ◽  
Vol 46 (2) ◽  
pp. 1895-1908
Author(s):  
Ramesh Kumar Kushwaha ◽  
Sucheta Singh ◽  
Shiv Shanker Pandey ◽  
Alok Kalra ◽  
Chikkarasanahalli Shivegowda Vivek Babu
Keyword(s):  
Cell Suspension ◽  
Endophytic Fungus ◽  
Withania Somnifera ◽  
Aspergillus Terreus ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Root Cell ◽  
Biotic Elicitor ◽  
Withanolide A

Establishment of Callus and Cell Suspension Cultures of Corydalis saxicola Bunting, a Rare Medicinal Plant

2006 ◽  
Vol 61 (3-4) ◽  
pp. 251-256 ◽  
Author(s):  
Hua Cheng ◽  
Long-Jiang Yu ◽  
Qiong-Yue Hu ◽  
Shan-Cai Chen ◽  
You-Ping Sun
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Large Scale ◽  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Biomass Accumulation ◽  
Suspension Cultures ◽  
Inoculum Size ◽  
Enhanced Production

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l−1 2,4-D plus 2 mg l−1 BA was the most favorable medium for callus formation with the highest induction rate (94.8%) and greatest fresh weight of callus (1.7 g per explant). Cell suspension cultures were established by transferring 2-8 g fresh callus to 80 ml liquid B5 medium. An inoculum size of 8 g produced the greatest biomass accumulation, dehydrocavidine and berberine productions, which was 13.1 g l−1, 8.0 mg l−1 and 4.1 mg l−1, respectively. In response to various sucrose concentrations from 10 g l−1 to 80 g l−1, cultures with 60 g sucrose l−1 not only produced the highest dry biomass (18.5 g l−1) but also the highest formation of dehydrocavidine (11.6 mg l−1) and berberine (7.6 mg l−1). These prepared cell suspension cultures provided a useful material for further regulation of alkaloid biosynthesis and for enhanced production of valuable alkaloids on a large scale.

Sign in / Sign up

Export Citation Format

Share Document