Metabolic evaluation of cellular differentiation of tobacco leaf explants in response to plant growth regulators in tissue cultures using 1H NMR spectroscopy and multivariate analysis

2011 ◽  
Vol 108 (2) ◽  
pp. 303-313 ◽  
Author(s):  
Suk Weon Kim ◽  
Jong Hyun Kim ◽  
Myung Suk Ahn ◽  
Dong-Ho Choung ◽  
Jang R. Liu
Keyword(s):  
Multivariate Analysis ◽  
Nmr Spectroscopy ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
1H Nmr Spectroscopy ◽  
Cellular Differentiation ◽  
Leaf Explants ◽  
Tissue Cultures ◽  
Metabolic Evaluation

scholarly journals Influence of Plant Growth Regulators and Medium Strength on Micropropagation of the Biodiesel Plant, Jatropha curcus

2016 ◽  
Vol 26 (1) ◽  
pp. 85-96 ◽  
Author(s):  
Mohamed R Rady ◽  
El Sayed ◽  
M Mervat ◽  
Mohamed El Sayed ◽  
Usama I Aly
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Jatropha Curcas ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Peat Moss ◽  
Medium Strength ◽  
Half Strength

Leaf explants were cultured to evaluate the effect of different auxins and cytokinins and its concentrations; MS salts on micropropagation of the promising biodiesel Jatropha curcas plant under Egyptian conditions. Results showed that shoot initiated on 0.5 mg/l BA + 0.25 mg/l IBA. Multiplication and elongation were found to be the best using 0.5 mg/l BA in combination with 0.05 mg/l IBA. The multiple shoots were cultured on MS or half strength of MS supplemented with different concentrations of IAA and IBA for rooting phase. Half strength of MS containing 1.0 mg/l IAA was the best for rooting of micropropagated shoots. The rooted plantlets were acclimated in sand : peat?moss mixture (1 : 1) successfully.Plant Tissue Cult. & Biotech. 26(1): 85-96, 2016 (June)

scholarly journals Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo (Solanum quitoense Lam.)

2014 ◽  
Vol 32 (2) ◽  
pp. 170-179 ◽  
Author(s):  
Hernando Criollo ◽  
Margarita Perea ◽  
Mariano Toribio ◽  
Johanna Muñoz
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Plant Propagation ◽  
Cotyledonary Leaf

Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. septentrionale and S. quitoense var. quitoense, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 mM of NAA were added to the medium with sucrose (50.0 and 263.1 mM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 mM of NAA ; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 mM of NAA + 263.1 mM of sucrose (32%).

scholarly journals Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP

2017 ◽  
Vol 9 (3) ◽  
pp. 402 ◽  
Author(s):  
Dwi Kusuma Wahyuni ◽  
Putri Andriani ◽  
Arif Nur Muhammad Ansori ◽  
Edy Setiti Wida Utami
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Culture Method ◽  
Dry Weight ◽  
Fresh Weight ◽  
Two Factors

<p class="IsiAbstrakIndo"><em>Justicia gendarussa </em>Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (<span>α</span> = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. <span lang="EN-GB">Moreover, t</span>he optimum treatment will be used to produce secondary metabolite and seed s<span lang="EN-GB">y</span>nthetic by cell suspension culture.</p>

Comparative Effects of Plant Growth Regulators on Callus Induction in Leaf Explants of Wedelia chinensis

2016 ◽  
Vol 2 (2) ◽  
pp. 320
Author(s):  
Brijit C. Dhas ◽  
Nimmi S. Simon ◽  
P. Austin ◽  
Sentamil Selvan ◽  
Shyam Kumar Rajaram ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants

scholarly journals Effects of different plant growth regulators on in vitro growth of Aloe vera L.

2020 ◽  
Vol 49 (1) ◽  
pp. 159-162
Author(s):  
Unaiza Wahab ◽  
Muhammad Ashfaq ◽  
Muhammad Sajjad ◽  
Shabnum Shaheen ◽  
Riffat Sadique ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Aloe Vera ◽  
Leaf Explants ◽  
Leaf Node ◽  
Ms Medium ◽  
In Vitro Growth

An attempt was made to standardize the appropriate concentration of different growth regulators for successful in vitro growth of different explants (leaf, node and internode) of Aloe vera L. Results demonstrated best in vitro growth in leaf explants in MS medium supplemented with BAP (1.0 mg/l) and NAA (1.0 mg/l) at 26 ± 2ºC) with pH 5.70 using agar solidified medium and 16 hrs photoperiod.

scholarly journals Biological Activity of the tzs Gene of Nopaline Agrobacterium tumefaciens GV3101 in Plant Regeneration and Genetic Transformation

2013 ◽  
Vol 26 (11) ◽  
pp. 1359-1365 ◽  
Author(s):  
Zhao-Fen Han ◽  
David M. Hunter ◽  
Susan Sibbald ◽  
Ji-Shu Zhang ◽  
Lining Tian
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Growth ◽  
Genetic Transformation ◽  
Plant Growth Regulators ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agrobacterium Strain

Agrobacterium tumefaciens has been widely used in plant genetic transformation. Hormone-encoding genes residing in the T-DNA region have been removed, resulting in disarmed Agrobacterium strains that are used in various transformation experiments. Nopaline Agrobacterium strains, however, carry another hormone gene, trans-zeatin synthesizing (tzs), that codes for trans-zeatin in the virulence region of the tumor-inducing plasmids. We investigated the activity and function of the tzs gene of a nopaline Agrobacterium sp. strain GV3101 in plant in vitro regeneration. Leaf explants of tobacco and Nicotiana benthamiana co-cultured with strain GV3101 exhibited active shoot regeneration in media without added plant growth regulators. On medium without plant growth regulators, transgenic shoots were also induced from explants co-cultured with GV3101 containing a binary vector. Enzyme-linked immunosorbent assay showed that cell-free extracts of Agrobacterium sp. strain GV3101 culture contained the trans-zeatin at 860 ng/liter. Polymerase chain reaction using tzs-specific primers showed that the tzs gene was present in strain GV3101 but not in other Agrobacterium strains. The study showed that the tzs gene in GV3101 was actively expressed, and that trans-zeatin produced in the Agrobacterium strain can promote plant shoot regeneration.

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