In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill

2012 ◽  
Vol 111 (3) ◽  
pp. 345-358 ◽  
Author(s):  
S. O. Amoo ◽  
A. O. Aremu ◽  
J. Van Staden
Keyword(s):  
Antioxidant Activity ◽  
Plant Regeneration ◽  
Secondary Metabolite ◽  
Secondary Metabolite Production ◽  
In Vitro Plant Regeneration ◽  
Metabolite Production ◽  
Aloe Arborescens ◽  
In Vitro Plant

scholarly journals High Efficiency in vitro Plant Regeneration and Secondary Metabolite Quantification from Leaf Explants of Rhodiola imbricata

2018 ◽  
Vol 10 (3) ◽  
pp. 470-475 ◽  
Author(s):  
Ashwani Kumar Bhardwaj ◽  
Avilekh Naryal ◽  
Pushpender Bhardwaj ◽  
Ashish Rambhau Warghat ◽  
Balpreet Arora ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Secondary Metabolite ◽  
High Efficiency ◽  
Leaf Explants ◽  
In Vitro Plant Regeneration ◽  
Metabolite Quantification ◽  
In Vitro Plant ◽  
Rhodiola Imbricata

scholarly journals Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

2021 ◽  
Vol 22 (11) ◽  
pp. 5671
Author(s):  
Mohsen Hesami ◽  
Austin Baiton ◽  
Milad Alizadeh ◽  
Marco Pepe ◽  
Davoud Torkamaneh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Engineering ◽  
Suspension Culture ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Transformation ◽  
Secondary Metabolite Production ◽  
Metabolite Production

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

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