scholarly journals Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

2021 ◽  
Author(s):  
Tushar Kashinath Manape ◽  
Viswanathan Satheesh ◽  
Shweta Singh ◽  
Major Singh ◽  
Sivalingam Anandhan
Keyword(s):  
Allium Cepa ◽  
Improved Method ◽  
Agrobacterium Mediated Transformation ◽  
Short Day ◽  
Allium Cepa L

scholarly journals Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

2021 ◽  
Author(s):  
TUSHAR KASHINATH MANAPE ◽  
Viswanathan Satheesh ◽  
Shweta Singh ◽  
Major Singh ◽  
Sivalingam Anandhan
Keyword(s):  
Callus Induction ◽  
Transformation Efficiency ◽  
Improved Method ◽  
Embryogenic Calli ◽  
Short Day ◽  
Allium Cepa L ◽  
Onion Cultivar ◽  
Embryogenic Callus Induction ◽  
High Auxin ◽  
Stable Transgenics

Abstract A high-auxin medium, usually used for callus induction, was not effective for Indian short-day onion cv. Bhima super. In this study, we found that the onion seedling radicle was a better explant than shoot tip for embryogenic callus induction, and induction efficiency up to 85.33% along with high embryogenic calli weight was obtained in routinely used medium containing 1.0 mg/L 2,4-D, but specifically supplemented with 0.5 mg/L kinetin. MS medium supplemented with 1.5 mg/L kinetin and 0.125 mg/L ABA showed 73.15% shoot regeneration efficiency from the calli induced from seedling radicle. Geneticin and hygromycin B at 50 mg/L showed optimal selection pressure for 8-week-old onion calli. Agrobacterium-mediated transformation of 8-week-old friable embryogenic calli induced from seedling radicle resulted in phenotypically normal transgenic plants with 1% transformation efficiency. In this study, regeneration and transformation protocols were developed for a widely used Indian short-day onion cultivar, which is instrumental for the development of stable transgenics in this crop.

scholarly journals PHOTOSYNTHESIS AND TRANSPIRATION DURING GROWTH AND DEVELOPMENT OF ALLIUM CEPA L.

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 263B-263
Author(s):  
Daniel Warnock ◽  
William Randle ◽  
Mark Rieger
Keyword(s):  
Water Use Efficiency ◽  
Water Use ◽  
Allium Cepa ◽  
Growth And Development ◽  
Developmental Stages ◽  
Leaf Conductance ◽  
Short Day ◽  
Allium Cepa L ◽  
Use Efficiency ◽  
Inductive Photoperiod

Photosynthesis is the very essence of agriculture. Previous photosynthetic and transpirational studies of onion (Allium cepa) have been limited to specific developmental stages. Our study measured photosynthesis and transpiration in sixteen plants of a single short-day cultivar over an eleven week period containing both non- and bulb inductive photoperiods. Differences in weekly means for photosynthesis, leaf conductance, water use efficiency, and intercellular CO, were highly significant. Weekly photosynthetic means increased under a non-inductive photoperiod and peaked one week after initiating a bulb inducing photoperiod. A decrease and leveling period occurred as bulbs developed followed by a decrease as foliage lodged. Weekly photosynthetic and leaf conductance means were correlated and highly significant. Water use efficiency and intercellular CO, means remained fairly constant throughout the study suggesting that photosynthesis in unstressed onions was controlled by internal mechanisms instead of stomata.

scholarly journals Expression analysis and association of bulbing to FLOWERING LOCUS T (FT) gene in short day onion (Allium cepa L.)

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
Fornesta Lyngkhoi ◽  
Anil Khar ◽  
Manisha Mangal ◽  
Ambika B. Gaikwad ◽  
Nepolean Thirunavukkarasu
Keyword(s):  
Gene Expression ◽  
Allium Cepa ◽  
Expression Analysis ◽  
Flowering Locus T ◽  
Short Day ◽  
Long Day ◽  
Expression Studies ◽  
Allium Cepa L ◽  
Flowering Locus ◽  
Six Genes

The present study was carried out with the objective of studying the expression of FLOWERING LOCUS T (FT) genes responsible for bulbing in onion. Six AcFT genes were used for expression studies at prebulbing (45 DAT), bulbing (60 DAT) and post bulbing (75 DAT) stage. Two varieties viz., Brown Spanish (long day) and Pusa Riddhi (short day) were used. It was observed that out of the reported six AcFT primers, four were able to amplify. Two new primers exhibiting amplification of AcFT1 and AcFT6 are reported. In short day onion variety, Pusa Riddhi, expression of five out of six genes studied (AcFT1, AcFT3, AcFT4, AcFT5 and AcFT6) were highest at the bulbing stage (60 DAT) suggesting their role in bulbing. In long day variety, Brown Spanish, expression of all the six genes except AcFT1 was very low at bulbing stage (60 DAT). This signifies that specific photoperiod conditions for gene expression were not achieved under Delhi conditions in long day onion. More studies towards understanding of the genes need to be addressed to understand the basic molecular process of bulbing in short day onion.

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