scholarly journals Improved method for regeneration and Agrobacterium-mediated transformation of Indian short-day onion (Allium cepa L.)

2021 ◽  
Author(s):  
TUSHAR KASHINATH MANAPE ◽  
Viswanathan Satheesh ◽  
Shweta Singh ◽  
Major Singh ◽  
Sivalingam Anandhan
Keyword(s):  
Callus Induction ◽  
Transformation Efficiency ◽  
Improved Method ◽  
Embryogenic Calli ◽  
Short Day ◽  
Allium Cepa L ◽  
Onion Cultivar ◽  
Embryogenic Callus Induction ◽  
High Auxin ◽  
Stable Transgenics

Abstract A high-auxin medium, usually used for callus induction, was not effective for Indian short-day onion cv. Bhima super. In this study, we found that the onion seedling radicle was a better explant than shoot tip for embryogenic callus induction, and induction efficiency up to 85.33% along with high embryogenic calli weight was obtained in routinely used medium containing 1.0 mg/L 2,4-D, but specifically supplemented with 0.5 mg/L kinetin. MS medium supplemented with 1.5 mg/L kinetin and 0.125 mg/L ABA showed 73.15% shoot regeneration efficiency from the calli induced from seedling radicle. Geneticin and hygromycin B at 50 mg/L showed optimal selection pressure for 8-week-old onion calli. Agrobacterium-mediated transformation of 8-week-old friable embryogenic calli induced from seedling radicle resulted in phenotypically normal transgenic plants with 1% transformation efficiency. In this study, regeneration and transformation protocols were developed for a widely used Indian short-day onion cultivar, which is instrumental for the development of stable transgenics in this crop.

scholarly journals Evaluation of the Genotype-dependency of the Leafbased Regeneration and Transformation System in Maize

2018 ◽  
Vol 28 (2) ◽  
pp. 261-268 ◽  
Author(s):  
Saeideh Ebrahimzadeh ◽  
Mohammad Ahmadabadi
Keyword(s):  
Tissue Culture ◽  
Genetic Transformation ◽  
Callus Induction ◽  
Gus Expression ◽  
Transformation System ◽  
Embryogenic Calli ◽  
Maize Genotypes ◽  
Explant Source ◽  
Young Leaves ◽  
Embryogenic Callus Induction

Tissue culture and genetic transformation in maize are very laborious. The existing regeneration methods, which mainly use immature embryos as starting material, are highly genotype-dependent. Leaf segments can be used as an alternative explant source to produce embryogenic calli. Although a reliable leafbased regeneration and transformation system has been recently reported for maize, however, the genotype-dependency of this method has not been described yet. To this end, we evaluated the production of embryogenic calli from young leaves of several maize genotypes. The results showed that, overall callus induction potential as well as embryogenic callus induction rate is significantly different among the tested genotypes, demonstrating the genotypedependency of this system. However, induced embryogenic calli from different genotypes remained their embryogenic capability during several callus multiplication rounds. In addition, embryogenic calli showed high potential for biolistic-based genetic transformation, as revealed by transient GUS expression. Plant Tissue Cult. & Biotech. 28(2): 261-268, 2018 (December)

scholarly journals Embryogenic Callus Induction and Regeneration of Elite Bangladeshi Indica Rice Cultivars

1970 ◽  
Vol 17 (1) ◽  
pp. 65-70 ◽  
Author(s):  
ME Hoque ◽  
MS Ali ◽  
NH Karim
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Indica Rice ◽  
Rice Cultivars ◽  
Embryogenic Calli ◽  
Rice Callus ◽  
Embryogenic Callus Induction ◽  
Embryogenic Callus Formation

Significant variations were observed among six elite Bangladeshi Indica rice cultivars tested in relation to total callus induction frequency (p = 0.017), embryogenic callus formation frequency (p = 0.001) and subsequent plant regeneration responses (p = 0.005). In all the cases, embryogenic callus formation frequency was much more less than the total callus (embryogenic + non-embryonegic) formation frequency. The embryogenic calli derived from mature seed embryos produced green plants, successfully established in soil and produced fertile seeds.Key words: Indica rice, Callus induction, Plant regeneration, Genotypic variationsDOI = 10.3329/ptcb.v17i1.1122Plant Tissue Cult. & Biotech. 17(1): 65-70, 2007 (June)

Embryogenic Callus Induction and Plant Regeneration in Triticum tauschii, the Diploid D-Genome Donor for Bread Wheat (Triticum aestivum)

1996 ◽  
Vol 44 (4) ◽  
pp. 489 ◽  
Author(s):  
S Afsharsterle ◽  
ECK Pang ◽  
JS Brown ◽  
JF Kollmorgen
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
D Genome ◽  
Triticum Tauschii ◽  
Embryogenic Callus Induction ◽  
2,4 Dichlorophenoxyacetic Acid

Immature embryos of seven accessions of Triticum tauschii (Coss.) Schmal. were used to produce embryogenic callus suitable for initiation of suspension cultures. Several modifications of Murashige and Skoog basal medium (MS) were evaluated for callus induction from scutellar tissues of embryos. Nodular, embryogenic calli were induced from all accessions when MS medium was supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and a mixture of L-glutamine, L-asparagine and L-proline. Early differentiation of these embryogenic calli was overcome by substituting Dicamba for 2,4-D. Addition of 575 mg L-1 of L-proline gave a rapid increase in the production of nodular embryogenic callus in most of the accessions. Using this protocol, the embryogenic capacity of this type of callus was maintained for more than a year following further modification of the MS medium. A clear genotype dependency as well as media effects on the production of callus were observed.

scholarly journals Regeneration of Indian maize genotypes (Zea mays L.) from immature embryo culture through callus induction

2015 ◽  
Vol 7 (1) ◽  
pp. 131-137 ◽  
Author(s):  
N. Malini ◽  
C. R. Ananadakumar ◽  
S. Hari Ramakrishnan
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Immature Embryo ◽  
Regeneration Ability ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Maize Hybrids ◽  
Maize Genotypes ◽  
Embryogenic Callus Induction

Callus induction and regeneration ability of five maize genotypes UMI 757, UMI 615, UMI 112, UMI 285 and CO 1 and one promising maize hybrids COH(M) 5 were investigated using 14 days old immature embryos as explants. Callus induction depends on genotype, explants (age and size of explants), medium, type of auxin and their concentration. Explants grown on Murashige and Skoog (MS) medium supplemented with 1.5 mg/l 2, 4 - D (2,4 – dichloro phenoxy acetic acid), 0.3 mg/l kinetin with 30 g/l maltose showed the highest percentage of embryogenic callus induction. Among the six genotypes tested, COH(M) 5 maize hybrids have highest percentage of embryogenic calli. The embryogenic calli incubated on MS medium supplemented with 1.5 mg/l BAP (Benzyl Amino Purine), 0.2 mg/lNAA (Naphthalene Acetic Acid) with 1.0 mg/l kinetin was found to give the highest organogenesis response and regeneration of plantlets.

scholarly journals In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Dwi Hapsoro ◽  
Rahmadyah Hamiranti ◽  
Yusnita Yusnita
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Leaf Explants ◽  
Regeneration Medium ◽  
Embryogenic Calli ◽  
Robusta Coffee ◽  
Primary Callus ◽  
Ascorbic Acids ◽  
Superior Clones

Abstract. Hapsoro D, Hamiranti R, Yusnita Y. 2020. In vitro somatic embryogenesis of superior clones of robusta coffee from Lampung, Indonesia: Effect of genotypes and callus induction media. Biodiversitas 21: 3811-3817. This study aimed to investigate the effects of genotypes and primary callus induction media on somatic embryogenesis of superior robusta coffee clones of Lampung. Leaf explants of clones Tugusari, Komari, Tugino, and Wanto were cultured on two types of primary callus induction media (PCIM). PCIM1 consisted of half-strength MS salts, 30 gL-1 sucrose, added with (mgL-1) 0.1 thiamine-HCl, 0.5 nicotinic acids, 0.5 pyridoxine-HCl, 100 Myo-inositol, 200 ascorbic acids, 150 citric acids, and 1 benzyl adenine. PCIM2 consisted of NPCM salts, 30 gL-1 sucrose, added with (mgL-1) 15 thiamine-HCl, 1 nicotinic acid, 1 pyridoxine-HCl, 2 glycines, 130 Myo-inositol, 200 ascorbic acids, 150 citric acids, 1 2,4-dichlorophenoxyacetic acid, and 2 thidiazuron. The highest percentage (100%) of primary callus formation was found in Komari and Wanto clones. PCIM2 resulted in more primary calli than PCIM1. When subcultured to embryogenic callus induction medium, primary calli of clone Komari and Wanto developed into a high percentage of embryogenic calli, while those of the other two turned brown and died. PCIM2-derived primary calli developed into more embryogenic calli. When subcultured on somatic embryo (SE) regeneration medium, these calli underwent the formation of SE of various stages. When subcultured to plant regeneration medium, these SEs developed into plantlets.

scholarly journals Embryogenic Callus Induction of Aquilaira Malaccensis Lam. and Aquilaria Subintegra Ding Hou

2019 ◽  
Vol 9 (1) ◽  
pp. 5746-5751
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Aseptic Culture ◽  
Aquilaria Malaccensis ◽  
The Family ◽  
Commercially Important ◽  
Embryogenic Callus Induction ◽  
2,4 Dichlorophenoxyacetic Acid

Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

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