scholarly journals Efficacy of two commercial synthetic pyrethroids (cypermethrin and deltamethrin) on Amblyomma variegatum and Rhipicephalus microplus strains of the south-western region of Burkina Faso

2021 ◽  
Vol 53 (3) ◽  
Author(s):  
Achille S. Ouedraogo ◽  
Olivier M. Zannou ◽  
Abel S. Biguezoton ◽  
Kouassi Yao Patrick ◽  
Adrien M. G. Belem ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Pyrethroid Resistance ◽  
Tick Control ◽  
Rhipicephalus Microplus ◽  
Amblyomma Variegatum ◽  
Synthetic Pyrethroid ◽  
Cattle Tick ◽  
Control Problems ◽  
Synthetic Pyrethroids ◽  
Western Region

AbstractSince 2011, period of the livestock invasion by the cattle tick Rhipicephalus microplus in Burkina Faso (BF), tick-control problems were exacerbated. Based on farmer’s reports, most commonly used commercial acaricides were found to be ineffective in Western South part of the country. To investigate the occurrence and extent of such acaricidal ineffectiveness, we performed the standardized larval packet test (LPT) with commercial deltamethrin (vectocid) and cypermethrin (cypertop), on two cattle tick species, the native Amblyomma variegatum and the invasive R. microplus. The resistance ratios (RR) were computed with susceptible Hounde strain of Rhipicephalus geigyi as reference. The R. microplus population showed resistance to the two acaricides tested with the highest lethal concentration (LC) values, and different resistance ratios higher than 4 (deltamethrin: RR50 = 28.18 and RR90 = 32.41; cypermethrin: RR50 = 8.79 and RR90 = 23.15). In the contrary, A. variegatum population was found to be highly susceptible to acaricides tested with low lethal concentrations and resistance ratio values (deltamethrin: RR50 = 0.5 and RR90 = 0.48; cypermethrin: RR50 = 0.68 and RR90 = 0.79). These data demonstrate high synthetic pyrethroid resistance in R. microplus strain, leading to conclude that the acaricide ineffectiveness in tick populations control remains a concern in BF.

scholarly journals Pyrethroid Acaricide Resistance Is Proportional to P-450 Cytochrome Oxidase Expression in the Cattle Tick Rhipicephalus microplus

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Raquel Cossío-Bayúgar ◽  
Francisco Martínez-Ibañez ◽  
Hugo Aguilar-Díaz ◽  
Estefan Miranda-Miranda
Keyword(s):  
Gene Expression ◽  
Pyrethroid Resistance ◽  
Linear Regression Analysis ◽  
Rhipicephalus Microplus ◽  
Cattle Tick ◽  
Synthetic Pyrethroids ◽  
Acaricide Resistance ◽  
Expression Levels ◽  
Field Isolates ◽  
Moderate Resistance

The goal of the present study was to assess the gene expression of xenobiotic metabolizing enzymes (XMEs) Cytochrome P-450 (CYP) and carboxylesterase (CE) related to detoxification of synthetic pyrethroids, plus acetylcholinesterase (AChE), in field isolates of acaricide-resistant Rhipicephalus microplus. The XMEs expression levels were assessed by mRNA measurement using quantitative reverse transcription PCR. The XME expression levels of field-isolated acaricide-resistant ticks were compared against acaricide-susceptible reference ticks used in this study as a gene expression baseline and represented as relative expression units (REU). Field isolates were subjected to toxicological bioassays and determined resistant to all the Pyr acaricides (Pyr), whereas most of them were found susceptible to organophosphorous acaricides (OP), with the exception of three isolates, which exhibited moderate resistance to Diazinon. Significantly higher levels of CYP were detected in pyrethroid-resistance ticks when compared to Su ticks (P<0.01). A linear regression analysis showed that pyrethroid acaricide resistance levels of R. microplus were proportional to the CYP expression levels (correlation coefficient (R):0.85; P<0.05). Analysis on CE expression levels showed only one isolate resistant to Pyr and OP with a statistically significant increase (P<0.01). AChE expression levels showed statistically significant (P<0.01) subexpression in all tick isolates when compared to the susceptible reference. Our results suggest that pyrethroid acaricide resistance in the cattle tick may be diagnosed by measuring the CYP expression levels using quantitative PCR.

Synthetic Pyrethroid, Natural Product, and Entomopathogenic Fungal Acaricide Product Formulations for Sustained Early Season Suppression of Host-Seeking Ixodes scapularis (Acari: Ixodidae) and Amblyomma americanum Nymphs

2020 ◽  
Author(s):  
Terry L Schulze ◽  
Robert A Jordan
Keyword(s):  
Natural Product ◽  
Ixodes Scapularis ◽  
Tick Control ◽  
Amblyomma Americanum ◽  
Synthetic Pyrethroid ◽  
Activity Period ◽  
Synthetic Pyrethroids ◽  
Seasonal Activity ◽  
Early Season ◽  
Host Seeking

Abstract We compared the ability of product formulations representing a synthetic pyrethroid acaricide (Talstar P Professional Insecticide), a natural product-based acaricide (Essentria IC3), and an entomopathogenic fungal acaricide (Met52 EC Bioinsecticide) to suppress Ixodes scapularis Say and Amblyomma americanum (L.) nymphs when applied following USEPA approved manufacturers’ label recommendations for tick control using hand-pumped knapsack sprayers before the beginning of their seasonal activity period in the spring. We applied Met52 EC Bioinsecticide (11% Metarhizium anisopliae Strain F52) to five 100 m2 plots (10.6 ml AI/plot) in mid-April 2020. Two weeks later at the end of April 2020, we treated an additional five 100 m2 plots each with either Talstar P Professional Insecticide (7.9% bifenthrin @ 2.5 ml AI/plot) or Essentria IC3 (10% rosemary oil, 5% geraniol, and 2% peppermint oil @ 86.6 ml AI/plot). Weekly sampling of all plots through the end of June 2020 showed that both Met52 EC Bioinsecticide and Essentria IC3 failed to maintain a 90% suppression threshold for I. scapularis, compared to control plots, and required two additional applications over the course of the trial. In contrast, Talstar P Professional Insecticide suppressed 100% of I. scapularis nymphs and ≥96 and 100% of A. americanum nymphs and adults, respectively. Such pre-season applications of synthetic pyrethroids significantly reduce the early season acarological risk for exposure to host-seeking ticks as well as the frequency of acaricide applications.

Exploiting the heterogeneous drop-off rhythm of Amblyomma variegatum nymphs to reduce pasture infestation by adult ticks

Parasitology ◽  
2010 ◽  
Vol 137 (7) ◽  
pp. 1129-1137 ◽  
Author(s):  
F. STACHURSKI ◽  
H. ADAKAL
Keyword(s):  
Burkina Faso ◽  
Control Strategy ◽  
Tick Control ◽  
Dry Season ◽  
Amblyomma Variegatum ◽  
Herd Management ◽  
Turn On ◽  
Tick Distribution ◽  
Tick Survival

SUMMARYTick distribution depends on the drop-off rhythm of the previous stage and on the suitability for tick survival of the environment where they are disseminated. Studies were implemented in Burkina Faso to assess detachment pattern of engorged Amblyomma variegatum nymphs. Experiments were carried out with naturally infested cattle kept in a paddock or monitored when grazing community pasture. In the pasture, 80% of the nymphs detached between 14.00 h and 17.00 h while less than 25% did so in the paddock. Further investigation was implemented to assess whether the density of adult ticks might be modified by herd management. During the early dry season, zebus grazed in 4 plots fenced in natural savannahs and fallows. Two of the plots were used in the morning and the two others in the afternoon. Six months later, zebus were put in these plots, in turn, on 9 occasions. The number of A. variegatum adults picked up by the cattle in each plot was highly variable: they captured more ticks in the plots installed on good lush savannah and 3-fold more ticks in those where the herd had grazed in the afternoon during the previous dry season. An integrated tick control strategy taking these results into account is proposed.

Cattle-tick control in Northern Nigeria: a field study of BHC, Sevin, toxaphene and ronnel used as sprays

1964 ◽  
Vol 54 (4) ◽  
pp. 633-641 ◽  
Author(s):  
R. J. Thorpe ◽  
P. Walker
Keyword(s):  
Field Study ◽  
Tick Control ◽  
Amblyomma Variegatum ◽  
Cattle Tick ◽  
Tick Population ◽  
Wet Season ◽  
Boophilus Annulatus ◽  
Degree Of Control ◽  
Savannah Zone ◽  
Cattle Tick Control

A field study was made of the effects of γ BHC, Sevin, toxaphene and ronnel (fenchlorphos) used as sprays on the numbers of ticks infesting cattle at Shika stockfarm in the Northern Guinea Savannah zone of Nigeria.The tick population included Boophilus annulatus (Say), B. decoloratus (Koch), Amblyomma variegatum (F.), Hyalomma truncatum Koch and Rhipiccphalus simus simus Koch, and its distribution on the host is described.Significance tests of treatment difference were carried out for initial kill and reinfestation rate. In both dry- and wet-season trials toxaphene proved to be the most effective ixodicide. A satisfactory degree of control was given by γ BHC but not by Sevin or ronnel.It is concluded that spraying at 21- and 7-day intervals during the dry and wet seasons, respectively, with either toxaphene or γ BHC will maintain a stable, satisfactory level of tick parasitism and premunity to tick-borne diseases in the herd.

scholarly journals Physiological evidence that three known mutations in the para-sodium channel gene confer cypermethrin knockdown resistance in Rhipicephalus microplus

2020 ◽  
Author(s):  
Raquel Cossío-Bayúgar ◽  
Estefan Miranda-Miranda ◽  
Francisco Martínez Ibañez ◽  
Verónica Narváez Padilla ◽  
Enrique Reynaud
Keyword(s):  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Experimental System ◽  
Rhipicephalus Microplus ◽  
Susceptible Strain ◽  
Cattle Tick ◽  
Knockdown Resistance ◽  
Channel Gene ◽  
Sodium Channel Gene ◽  
Physiological Evidence

Abstract Background: Acaricide resistance is a central problem for the control of the cattle tick Rhipicephalus microplus . Genetic changes that confer resistance get fixed in the population, however, the physiological effects and phenotypes of these mutations are not always well understood or characterized. SNPs in the para-sodium channel gene that confer cypermethrin knockdown resistance ( kdr ) were identified in several arthropods, and homologous mutations have been reported in R. microplus populations. To our knowledge, R. microplus populations that have these homologous SNPs have been associated and correlated with pyrethroid resistance but there is no direct physiological evidence that their presence does confer kdr in this organism. Methods: Resistance profiles from resistant and susceptible strains used in this work were obtained using the larval packet discriminating dose assay. The relevant genomic regions of the para-sodium channel of these strains were amplified using standard PCR; SNPs were detected by sequencing the corresponding amplicons. Ovary response to cypermethrin exposure/treatment was evaluated using videometrical analysis. Results: In this work we present historical evidence that the pyrethroid resistance trait is stable in a resistant reference strain after many years without selection, thus suggesting that the primary resistance mechanism is caused by mutations fixed in the population. In our experimental system, the mechanism that allows the maintenance of the contraction of the ovary after treatment with pyrethroids, is likely to be mediated by a change in the structure of the presynaptic para-sodium channel. We found that the resistant strain has the G184C, the C190A and the T2134A mutations in the para-sodium channel gene. SNPs G184C and T2134A have been reported to be genetically linked in resistant populations and are always found together. These mutations were confirmed to be absent in the susceptible strain used as control. Finally, using videometric analysis, we demonstrate that cypermethrin blocks ovary contraction in cypermethrin-susceptible ticks. We also show that ovaries from populations that carry the kdr associated SNPs still contract at cypermethrin concentrations that completely block ovary contraction in the susceptible strain. The configuration of the experimental system excludes a xenobiotic detoxification mechanism. Conclusions: This is the first report that presents physiological evidence that the presence of the G184C, the C190A, and the T2134A mutations in the para-sodium channel correlates with the ability of maintaining muscle contractility in R. microplus when exposed to cypermethrin. These results confirm that these SNPs may confer cypermethrin resistance in this organism by avoiding presynaptic blockage that in turn causes flaccid muscle paralysis that is characteristic of this acaricide. This work also demonstrates that the videometric assay that we previously validated can be used to detect more rapidly than other assays that involve larval mortality kdr -like cypermethrin resistant tick strains, because adult preengorged females can be directly assayed after they are collected on the field without waiting until eggs are laid and the larvae eclose.

scholarly journals Characterization of the pyrethroid resistance profile of Rhipicephalus (Boophilus)microplus populations from the states of Rio Grande do Sul and Mato Grosso do Sul, Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Márcia Cristina Mendes ◽  
Fernanda Calvo Duarte ◽  
João Ricardo Martins ◽  
Guilherme Marcondes Klafke ◽  
Leonardo Costa Fiorini ◽  
...  
Keyword(s):  
Pyrethroid Resistance ◽  
Rio Grande ◽  
Boophilus Microplus ◽  
Cattle Tick ◽  
Synthetic Pyrethroids ◽  
Rio Grande Do Sul ◽  
Mato Grosso ◽  
Cattle Ticks ◽  
Mato Grosso Do Sul

Cattle ticks Rhipicephalus(Boophilus) microplus are mainly controlled in Brazil by means of acaricide products, without any official policies in this regard. Acaricides continue to be sold indiscriminately, and this has contributed towards making the problem of resistance widespread, thus making diagnosis and monitoring of tick resistance essential. Here, bioassays (larval packet test) were performed on tick populations from the states of Rio Grande do Sul and Mato Grosso do Sul regarding their susceptibility to cypermethrin, deltamethrin and flumethrin. All the tick samples tested showed resistance to cypermethrin (10) (resistance factor (RF) ranging from 5.6 to 80.3) and deltamethrin (10) (RF ranging from 2.4 to 83.1). Six out of eight populations were resistant to flumethrin (RF ranging from 3.8 to 8.2). PCR molecular analyses did not show any T2134A mutations in the voltage-gated sodium channel gene, in any of the sampled populations. The results from this study highlight the critical status of resistance of the cattle tick to synthetic pyrethroids in the regions studied. Further studies are needed to identify the mechanisms responsible for the resistant phenotypes observed in the bioassays. This was the first detection of flumethrin resistance in Brazil.

Alarming spread of the Asian cattle tick Rhipicephalus microplus in West Africa—another three countries are affected: Burkina Faso, Mali and Togo

2013 ◽  
Vol 61 (3) ◽  
pp. 383-386 ◽  
Author(s):  
H. Adakal ◽  
A. Biguezoton ◽  
S. Zoungrana ◽  
F. Courtin ◽  
E. M. De Clercq ◽  
...  
Keyword(s):  
West Africa ◽  
Burkina Faso ◽  
Rhipicephalus Microplus ◽  
Cattle Tick

scholarly journals Sensitivity of Different Cattle Breeds to the Infestation of Cattle Ticks Amblyomma variegatum, Rhipicephalus microplus, and Hyalomma spp. on the Natural Pastures of Opkara Farm, Benin

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Roland Eric Yessinou ◽  
Camus Adoligbe ◽  
Yao Akpo ◽  
Justin Adinci ◽  
Issaka Youssao Abdou Karim ◽  
...  
Keyword(s):  
Tick Species ◽  
Tick Infestation ◽  
Tick Control ◽  
Rhipicephalus Microplus ◽  
Amblyomma Variegatum ◽  
Cattle Farm ◽  
Cattle Breeds ◽  
Cattle Ticks ◽  
Significant Difference ◽  
The Mean

A study was carried out on the Opkara (Benin) cattle farm on 64 cattle of four different breeds (16 individuals per breed) from June to December 2016. During this study, three tick species were found in different numbers, Amblyomma variegatum (732), Rhipicephalus microplus (8079), and Hyalomma spp. (208), with parasitic intensity of 11.90, 126.23, and 3.25, respectively. The interracial comparison of the tick infestation between the cattle showed a significant difference (P<0.001). However, Girolando was more infested than all the cattle breeds. Infestation of A. variegatum, R. microplus, and Hyalomma spp. on the Girolando was, respectively, 19.43 ± 2.71, 171.25 ± 23.50, and 7.12 ± 0.63, but the Borgou were less infested. Borgou breed females were more infested by A. variegatum (4.41 ± 1.14) than females Girolando (4.20 ± 0.90). The Crossbred and Azawak females were less infested (P<0.01). The mean of A. variegatum on Borgou, Azawak, Crossbred, and Girolando calves was 1.29 ± 0.35, 0.66 ± 0.26, 1.37 ± 0.37, and 2.25 ± 0.48 (P<0.01), respectively. The results of this study can be exploited to include genetic and nongenetic approaches to tick control.

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