Molecular cloning, genetic organization of gene cluster encoding phenol hydroxylase and catechol 2,3-dioxygenase in Alcaligenes faecalis IS-46

2008 ◽  
Vol 24 (9) ◽  
pp. 1687-1695 ◽  
Author(s):  
Chenguang Zhu ◽  
Liye Zhang ◽  
Liping Zhao
Keyword(s):  
Molecular Cloning ◽  
Gene Cluster ◽  
Alcaligenes Faecalis ◽  
Phenol Hydroxylase ◽  
Genetic Organization

scholarly journals New Insights into the Genetic Organization of the FK228 Biosynthetic Gene Cluster inChromobacterium violaceumNo. 968

2010 ◽  
Vol 77 (4) ◽  
pp. 1508-1511 ◽  
Author(s):  
Vishwakanth Y. Potharla ◽  
Shane R. Wesener ◽  
Yi-Qiang Cheng
Keyword(s):  
Natural Product ◽  
Gene Cluster ◽  
Gene Deletion ◽  
Regulatory Gene ◽  
Biosynthetic Gene Cluster ◽  
Transcriptional Analysis ◽  
Biosynthetic Gene ◽  
Genetic Organization

ABSTRACTThe biosynthetic gene cluster of FK228, an FDA-approved anticancer natural product, was identified and sequenced previously. The genetic organization of this gene cluster has now been delineated through systematic gene deletion and transcriptional analysis. As a result, the gene cluster is redefined to contain 12 genes:depAthroughdepJ,depM, and a newly identified pathway regulatory gene,depR.

Molecular cloning and characterization of an ML-236B (compactin) biosynthetic gene cluster in Penicillium citrinum

2002 ◽  
Vol 267 (5) ◽  
pp. 636-646 ◽  
Author(s):  
Y. Abe ◽  
T. Suzuki ◽  
C. Ono ◽  
K. Iwamoto ◽  
M. Hosobuchi ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Penicillium Citrinum ◽  
Biosynthetic Gene

scholarly journals Organization and Regulation of metaCleavage Pathway Genes for Toluene and o-Xylene Derivative Degradation in Pseudomonas stutzeri OX1

2001 ◽  
Vol 67 (7) ◽  
pp. 3304-3308 ◽  
Author(s):  
Fabio L. G. Arenghi ◽  
Davide Berlanda ◽  
Enrica Galli ◽  
Guido Sello ◽  
Paola Barbieri
Keyword(s):  
Gene Cluster ◽  
Gene Order ◽  
Pseudomonas Stutzeri ◽  
Phenol Hydroxylase ◽  
Catabolic Pathway ◽  
Semialdehyde Dehydrogenase

ABSTRACT Pseudomonas stutzeri OX1 meta pathway genes for toluene and o-xylene catabolism were analyzed, and loci encoding phenol hydroxylase, catechol 2,3-dioxygenase, 2-hydroxymuconate semialdehyde dehydrogenase, and 2-hydroxymuconate semialdehyde hydrolase were mapped. Phenol hydroxylase converted a broad range of substrates, as it was also able to transform the nongrowth substrates 2,4-dimethylphenol and 2,5-dimethylphenol into 3,5-dimethylcatechol and 3,6-dimethylcatechol, respectively, which, however, were not cleaved by catechol 2,3-dioxygenase. The identified gene cluster displayed a gene order similar to that of thePseudomonas sp. strain CF600 dmp operon for phenol catabolism and was found to be coregulated by thetou operon activator TouR. A hypothesis about the evolution of the toluene and o-xylene catabolic pathway inP. stutzeri OX1 is discussed.

Genetic Organization of the Escherichia coli K10 Capsule Gene Cluster: Identification and Characterization of Two Conserved Regions in Group III Capsule Gene Clusters Encoding Polysaccharide Transport Functions

1999 ◽  
Vol 181 (7) ◽  
pp. 2279-2285 ◽  
Author(s):  
Bradley R. Clarke ◽  
Rowan Pearce ◽  
Ian S. Roberts
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
Gene Clusters ◽  
Group Iii ◽  
Genetic Organization ◽  
Conserved Regions ◽  
Cluster Identification ◽  
Capsule Production ◽  
Identification And Characterization

ABSTRACT Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K10 capsule production, suggesting that expression of the K10 capsule was regulated by RfaH in a manner analogous to group II capsule gene clusters. An IS3 element and a φR73-like prophage, both of which may have played a role in the acquisition of group III capsule gene clusters, were detected flanking the K10 capsule genes.

Sign in / Sign up

Export Citation Format

Share Document