Geographic variations and their impact on bioefficacy amongst Helicoverpa armigera Nuclear Polyhedrosis Virus isolates from India

2009 ◽  
Vol 26 (5) ◽  
pp. 783-794 ◽  
Author(s):  
Charmi S. Patel ◽  
Janardan Jitendra Jani ◽  
Vipulkumar B. Parekh ◽  
Vijay B. Darji ◽  
Piyush R. Vaishnav
Keyword(s):  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Geographic Variations ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Virus Isolates

Genetic diversity and differentiation of Helicoverpa armigera nuclear polyhedrosis virus isolates from India

2009 ◽  
Vol 37 (5) ◽  
pp. 407-413 ◽  
Author(s):  
Charmi Shailesh Patel ◽  
Janardan J. Jani ◽  
Vipulkumar B. Parekh ◽  
Vijay B. Darji ◽  
Piyush R. Vaishnav
Keyword(s):  
Genetic Diversity ◽  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Virus Isolates

scholarly journals DAYA PATOGENESITAS Helicoverpa armigera Nuclear Polyhedrosis Virus HaNPV SETELAH TERKENA RADIASI SINAR ULTRAVIOLET

2000 ◽  
Vol 6 (1) ◽  
pp. 65-70
Author(s):  
Mahanani Tri Asri ◽  
Isnawati Isnawati
Keyword(s):  
Biological Control ◽  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Uv Light ◽  
Ultra Violet ◽  
Ultra Violet Light ◽  
Violet Light ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

This research is about pathogen capacity of the Helicoverpa armigera nuclear Polyhedrosis Virus (HaNPV) after irradiation by ultra violet (UV) light. The HaNPV is the one kind of the virus which attack the insect Helicoverpa armigera-attacker the some species of the crop life tobacco, cotton, potato, tomato. Because of that the HaNPV can be used as the biological control of the pest. Some researcher stated that the pathogen capacity of the HaNPV is decrease if the virus was expose in the ultra violet (UV) light. Because of the fact the virus in not useful as the biological control of the pest in the land corp. This research to test the statement. Laboratory experimental was done to tested the pathogen capacity of the HaNPV after irradiation treatment by the ultra violet light. The intensity of the UV light in this experiment were 28.7 lux, 97.3 lux, and 127.4 lux with the lighting period were 0, 1, 3, 6, 9, 12, and 15. The result of the research stated that the pathogen capacity of the HaNPV was not influenced by ultra violet light. So the virus is still useful as the biological control of the pest in the land crop.

scholarly journals Biological Test of Formulation of Subculture Helicovepa armigera Nuclear Polihedrosis Virus (HaNPV) on Mortality of Spodoptera litura Larvae infested to cabbage (Brassica oleracea Var. capitata Linn.) Plantation

2015 ◽  
Vol 2 (1) ◽  
pp. 646
Author(s):  
Desy Efriyani Anggraeny Nasution ◽  
Mia Miranti ◽  
Melanie .
Keyword(s):  
Brassica Oleracea ◽  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Insect Pests ◽  
Block Design ◽  
Economic Value ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>

scholarly journals EFISIENSI PENGENDALIAN PENGGEREK BUAH KAPAS Helicoverpa armigera HÜBNER DENGAN SERBUK BIJI MIMBA DAN NUCLEAR POLYHEDROSIS VIRUS

2020 ◽  
Vol 12 (2) ◽  
pp. 45
Author(s):  
I G.A.A. INDRAYANI ◽  
DWI WINARNO ◽  
TEGER BASUKI BASUKI
Keyword(s):  
Helicoverpa Armigera ◽  
Nuclear Polyhedrosis Virus ◽  
Rate Of Return ◽  
Recommended Dose ◽  
Neem Seed ◽  
Marginal Rate ◽  
Helicoverpa Armígera ◽  
Marginal Rate Of Return ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

ABSTRAK<br />Pengendalian hama non-kimiawi semakin meningkat sehingga<br />mengurangi penggunaan insektisida kimia. Alternatif pengendalian hama<br />menggunakan pestisida botani dan agensia mikrobia cukup efektif<br />mengendalikan penggerek buah kapas H. armigera. Penelitian efisiensi<br />pengendalian penggerek buah kapas H. armigera dengan SBM dan NPV<br />dilakukan di Kebun Percobaan Balai Penelitian Tanaman Tembakau dan<br />Serat di Asembagus, Situbondo, Jawa Timur mulai Januari hingga<br />Desember 2003. Tujuannya adalah untuk mengetahui tingkat efisiensi<br />pengendalian penggerek buah kapas H. armigera terutama dengan<br />kombinasi SBM dan NPV. Perlakuan yang digunakan adalah: (1)<br />SBM(LC 25 )+NPV(LC 50 ), (2) SBM(LC 50 )+NPV(LC 50 ), (3) SBM (dosis<br />rekomendasi), (4) NPV (dosis rekomendasi), (5) betasiflutrin (dosis<br />rekomendasi), dan (6) kontrol (tanpa perlakuan). Setiap perlakuan disusun<br />dalam rancangan acak kelompok (RAK) dengan tiga ulangan. Parameter<br />yang diamati meliputi populasi ulat H. armigera dan kompleks<br />predatornya, frekuensi penyemprotan masing-masing perlakuan, kerusakan<br />kuncup bunga dan buah kapas, biaya pengendalian hama, pendapatan,<br />marginal rate of return (MRR), dan hasil kapas serta kacang hijau. Hasil<br />penelitian menunjukkan bahwa pengendalian H. armigera dengan<br />kombinasi perlakuan SBM(LC 50 )+NPV(LC 50 ) lebih efisien menurunkan<br />biaya pengendalian hama hingga 63,4% dan meningkatkan pendapatan<br />sebesar 32,7% dibanding insektisida kimia betasiflutrin, dengan nilai MRR<br />4,66 dan 4,28 masing-masing atas kontrol dan insektisida kimia.<br />Kata kunci: Kapas, Gossypium hirsutum, hama, penggerek buah,<br />Helicoverpa armigera, SBM, NPV, pengendalian hama,<br />marginal rate of return, Jawa Timur<br />ABSTRACT<br />Efficiency in cotton bollworm Helicoverpa armigera Hübner control<br />using neem seed powder and nuclear polyhedrosis virus<br />Insect pest biological control potentially decreases the use of<br />chemical insecticides. The alternative control method chosen was<br />combination of botanical and microbial agents that showed higher<br />effectiveness against H. armigera. This control method might also<br />potential to minimize the use of chemical pesticide on cotton. Study on<br />efficiency of cotton bollworm Helicoverpa armigera Hübner control using<br />neem seed powder (NSP) and nuclear polyhedrosis virus was conducted at<br />Asembagus Experimental Station of Indonesian Tobacco and Fiber Crops<br />Research Institute, Situbondo, East Java, from January to December 2003.<br />The objective of this study was to find out the efficiency level of cotton<br />bollworm control using combination of neem seed powder (NSP) and<br />nuclear polyhedrosis virus (NPV). The treatments were : (1) NSP(LC 25 ) +<br />NPV(LC 50 ), (2) NSP(LC 50 ) + NPV(LC 50 ), (3) NSP (recommended dose),<br />(4) NPV (recommended dose), (5) betacyfluthrin (recommended dose),<br />and (6) control (untreated). The treatments were arranged in a randomized<br />block design with three replications. Parameters observed were population<br />of H. armigera larvae and its complex predators, frequency of spraying,<br />square and boll damage, cost of control, net income, marginal rate of<br />return, and yield of seed cotton and mungbean. The research results<br />showed that the combination of NSP(LC 50 )+NPV(LC 50 ) effectively<br />reduced the total cost of insect control by 63.4% and increased the increase<br />32.7% compared to betacyfluthrin. The combination also showed the<br />highest marginal rate of return of 4.66 and 4.28 based on control and<br />betacyfluthrin, respectively.<br />Key words: Cotton, Gossypium hirsutum, insect, cotton bollworm,<br />Helicoverpa armigera, NSP, NPV, insect control, marginal<br />rate of return, East Java

scholarly journals Storage Stability and Performance of Aqueous and Dry Formulations of Helicoverpa armigera Nuclear Polyhedrosis Virus

2016 ◽  
Vol 30 (1) ◽  
pp. 34
Author(s):  
P. N. Mane ◽  
M. P. Moharil ◽  
N. S. Satpute ◽  
S. M. Thakare ◽  
G. K. Giri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Shelf Life ◽  
Helicoverpa Armigera ◽  
Storage Stability ◽  
Nuclear Polyhedrosis Virus ◽  
Storage Period ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
And Performance ◽  
Nuclear Polyhedrosis

Experiment on storage stability and performance of aqueous and dry formulation of <em>Helicoverpa armigera</em> nuclear polyhedrosis virus (HaNPV) was conducted during 2011-2013 in Bio control laboratory, Department of Entomology and Insect Biotechnology laboratory, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola with an objective to develop potent HaNPV formulation for improving shelf life and performance. Aqueous and dry form of HaNPV formulations with antimicrobials, UV protectant and phagostimulant were prepared and studied their shelf life and performance during storage. Synthesized and characterized the Silver nanoparticles. Prepared fresh Ha NPV required for preparing different formulation. The contaminants associated, storage stability and larvicidal activity of formulated HaNPV was studied. Data revealed that, HaNPV formulations having Silver nanoparticles @ 8 μl/ml of HaNPV and 80 μl/ml of HaNPV checked the bacterial contamination up to 13 months of the storage period, did not affect the viability of POBs and insecticidal properties of HaNPV formulation. Aqueous form of HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + Tinopal 1% + Sucrose 1% , dry form of HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + Tinopal 1% + Sucrose 1% and HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + sucrose remain stabled up to 12 months. However, aqueous form of HaNPV alone reduces the insecticidal properties from the 9th month of storage period. While, dry form of HaNPV alone reduces the insecticidal properties from the 12th month of storage period. From the result, it was noticed that dry form of HaNPV formulation having antimicrobial found more stable than aqueous form.

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