Factors affecting antifungal activity of Streptomyces philanthi RM-1-138 against Rhizoctonia solani

2013 ◽  
Vol 30 (1) ◽  
pp. 323-329 ◽  
Author(s):  
Sawai Boukaew ◽  
Poonsuk Prasertsan
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Factors Affecting ◽  
Streptomyces Philanthi

scholarly journals Activity of Alkanediol Alkanoates against Pathogenic Plant Fungi Rhizoctonia solani and Sclerotium rolfsii

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Paraj Shukla ◽  
Suresh Walia ◽  
Vivek Ahluwalia ◽  
Balraj S. Parmar ◽  
Muraleedharan G. Nair
Keyword(s):  
Antifungal Activity ◽  
Ethylene Glycol ◽  
Rhizoctonia Solani ◽  
Diethylene Glycol ◽  
Sclerotium Rolfsii ◽  
Phytopathogenic Fungi ◽  
Dependent Manner ◽  
Acyl Chlorides ◽  
Mass Spectral ◽  
Dose Dependent

Thirty known dialkanoates of ethylene, propylene and diethylene glycols were synthesized by reacting the glycols with acyl chlorides and their structures confirmed by IR, NMR and mass spectral analyses. They exhibited significant antifungal activity against two phytopathogenic fungi Rhizoctonia solani Kuehn and Sclerotium rolfsii Sacc in a dose dependent manner. Propylene glycol dipentanoate was the most active against R. solani. followed by diethylene glycol dibutanoate and ethylene glycol dibutanoate. Against S. rolfsii ethylene glycol diheptanoate was found to be most active followed by diethylene glycol diisobutanoate As compared to the standard reference benomyl (EC50 5.16 μg/mL), the potential alkanediol dialkanoates showed EC50 in the range of 33 – 60 μg/mL.

Tricholoma matsutake fruit bodies secrete hydrogen peroxide as a potent inhibitor of fungal growth

2015 ◽  
Vol 61 (6) ◽  
pp. 447-450 ◽  
Author(s):  
Yoshimitsu Takakura
Keyword(s):  
Hydrogen Peroxide ◽  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Microbial Communities ◽  
Potent Inhibitor ◽  
Fungal Growth ◽  
Ectomycorrhizal Fungus ◽  
Tricholoma Matsutake ◽  
Pyranose Oxidase

Tricholoma matsutake is an ectomycorrhizal fungus that dominates the microbial communities in the soil of pine and spruce forests. The mycorrhizas of this fungus have antimicrobial activity, although factors responsible for the antimicrobial activity have not been fully elucidated. The present study shows that fruit bodies of T. matsutake secreted hydrogen peroxide (H2O2), which was produced by pyranose oxidase, and that the H2O2 thus secreted strongly inhibited the growth of mycelia of the phytopathological fungus Rhizoctonia solani. These findings suggest that fruit bodies of T. matsutake have antifungal activity and that the pyranose oxidase plays an important role in the antifungal activity.

Two mutants selectively resistant to polyenes reveal distinct mechanisms of antifungal activity by nystatin and amphotericin B

2005 ◽  
Vol 33 (5) ◽  
pp. 1206-1209 ◽  
Author(s):  
I. Hapala ◽  
V. Klobučníková ◽  
K. Mazáňová ◽  
P. Kohút
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Fungal Infections ◽  
Structural Similarity ◽  
Resistant Mutant ◽  
Factors Affecting ◽  
Polyene Macrolides ◽  
Mode Of Inheritance ◽  
Nystatin Resistance

Polyene macrolides nystatin and amphotericin B are widely used in the treatment of fungal infections. In order to characterize factors affecting polyene activity, we have isolated Saccharomyces cerevisiae mutants showing selective resistance to nystatin and amphotericin B. Characterization of two of these mutants (nystatin-resistant mutant X1/16 and amphotericin B-resistant mutant X3/33) is presented. Genetic analysis revealed that resistance in each of these mutants is caused by a mutation in one gene with a different mode of inheritance. Nystatin resistance in mutant X1/16 is caused by changes in sterol spectrum while amphotericin B resistance in mutant X3/33 is probably related to modification of the cell wall. Our results suggest that, in spite of their structural similarity, nystatin and amphotericin B differ significantly in mechanisms of their antifungal activity.

scholarly journals Factors affecting protoplast formation by Rhizoctonia solani

2010 ◽  
Vol 27 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Tung-Hsen Liu ◽  
Mei-Ju Lin ◽  
Wen-Hsiung Ko
Keyword(s):  
Rhizoctonia Solani ◽  
Protoplast Formation ◽  
Factors Affecting

scholarly journals Anti –phytopathogenic Activities of Cladophora glomerata extract against plant fungi

2014 ◽  
Vol 11 (2) ◽  
pp. 883-887 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Antimicrobial Agents ◽  
Methanol Extract ◽  
Cladophora Glomerata ◽  
Damping Off ◽  
Natural Antimicrobial ◽  
Plant Fungi ◽  
Pharmaceutical Industries ◽  
Crude Methanol Extract

The antiphytopathogenic effects of the crude methanol extract of Cladophora glomerata (Lin.) Kützing (Cladophoraceae) which isolated from Al Rashidiya region at north of Baghdad was investigated against two type of plant fungi (Pythium altimum and Rhizoctonia solani) which causes damping off disease where isolated from covered cucumber field in Al-Alyosifia region. Hot methanol extract showed antifungal activity against the two species of fungi in different concentrations(10,25,50 mg/ml) of extract to Cladophora glomerata as percentage inhibition (51.63 ,72.8 ,83.71) and (56.18 ,77.41 ,100)comparing with Pythium altimum and Rhizoctonia solani respectively .primary detection of active compounds showed that macroalgae (Cladophora glomerata) containing flavonoids,alkaloids, phenols and tannins. Our findings suggest the possibility of using the Cladophora glomerata as a novel source of natural antimicrobial agents for pharmaceutical industries.

scholarly journals Effects of some microelements on antifungal activity and biomass of the Actinomyces producing Validamycin-A

2018 ◽  
Vol 9 (3) ◽  
pp. 128-131
Author(s):  
Huu Thanh Luong ◽  
Thuy Nga Vu ◽  
Thi Thuy Ha ◽  
Kieu Bang Tam Nguyen ◽  
Thi Hong Van Dao
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Streptomyces Hygroscopicus ◽  
Validamycin A ◽  
Fungal Activity ◽  
Anti Fungal

Validamycin A (Val-A) is an aminoglycoside's antibiotic with anti-fungal activity. Val-A 
synthesized by Streptomyces hygroscopicus strain depending on the growth and development of this actinomyces. In this study, the effects of Mn and Zn on the antifungal activity and biomass of the Streptomyces hygroscopicus were conducted. The results showed that micronutrients Mn, Zn had significant effects on biomass as well as antifungal activity of strain Streptomyces hygroscopicus-DA15. With the addition of Mn at a concentration of 1μg/l of the nutrient medium, biomass of Streptomyces hygroscopicus was 2.85 ± 0.02 g/ml, the anti-fungal Rhizoctonia solani (R. solani) round diameter reached 3.5 ± 0.2 cm. With the addition of Zn = 3 µg/l of the nutrient medium, biomass of Streptomyces hygroscopicus DA15 was 4.5 ± 0.02 g/ml, the anti-fungal R. solani diameter reached 3.4 ± 0.2 cm. Validamycin A (val-A) là một loại kháng sinh có khả năng kháng nấm, được tổng hợp bởi xạ khuẩn Streptomyces hygroscopicus và phụ thuộc vào quá trình sinh trưởng, phát triển của xạ khuẩn. Bài báo này đánh giá ảnh hưởng của nguyên tố vi lượng Mn, Zn đến hoạt tính kháng nấm Rhizoctonia solani (R. solani) và sinh khối của chủng Streptomyces hygroscopicus DA15. Khi bổ sung Mn vào môi trường nuôi cấy với nồng độ 1μg/l, sinh khối của Streptomyces hygroscopicus-DA15 đạt 2,85±0,02g/ml, đường kính vòng kháng nấm đạt 3,5±0,2cm. Bổ sung Zn vào môi trường nuôi cấy với hàm lượng Zn=3µg/l, sinh khối của Streptomyces hygroscopicus DA15 đạt 4,5±0,02g/ml và đường kính vòng kháng nấm đạt 3,4±0,2cm.

scholarly journals Acaconin, a chitinase-like antifungal protein with cytotoxic and anti-HIV-1 reverse transcriptase activities from Acacia confusa seeds.

2010 ◽  
Vol 57 (3) ◽  
Author(s):  
Sze Kwan Lam ◽  
Tzi Bun Ng
Keyword(s):  
Antifungal Activity ◽  
Reverse Transcriptase ◽  
Rhizoctonia Solani ◽  
Fungal Growth ◽  
Chitinase Activity ◽  
Antifungal Protein ◽  
Hiv Reverse Transcriptase ◽  
Acacia Confusa ◽  
Congo Red Staining ◽  
Hiv 1

From the seeds of Acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward Rhizoctonia solani with an IC₅₀ of 30±4 µM was isolated. Acaconin demonstrated an N-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kDa. It was isolated by chromatography on Q-Sepharose, SP-Sepharose and Superdex 75 and was not bound by either ion exchanger. Acaconin was devoid of chitinase activity. The antifungal activity against Rhizoctonia solani was completely preserved from pH 4 to 10 and from 0°C to 70°C. Congo Red staining at the tips of R. solani hyphae indicated inhibition of fungal growth. However, there was no antifungal activity toward Mycosphaerella arachidicola, Fusarium oxysporum, Helminthosporium maydis, and Valsa mali. Acaconin inhibited proliferation of breast cancer MCF-7 cells with an IC₅₀ of 128±9 µM but did not affect hepatoma HepG2 cells. Its IC₅₀ value toward HIV-1 reverse transcriptase was 10±2.3 µM. The unique features of acaconin include relatively high stability when exposed to changes in ambient pH and temperature, specific antifungal and antitumor actions, potent HIV-reverse transcriptase inhibitory activity, and lack of binding by strongly cationic and anionic exchangers.

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