Activity of Alkanediol Alkanoates against Pathogenic Plant Fungi Rhizoctonia solani and Sclerotium rolfsii

Thirty known dialkanoates of ethylene, propylene and diethylene glycols were synthesized by reacting the glycols with acyl chlorides and their structures confirmed by IR, NMR and mass spectral analyses. They exhibited significant antifungal activity against two phytopathogenic fungi Rhizoctonia solani Kuehn and Sclerotium rolfsii Sacc in a dose dependent manner. Propylene glycol dipentanoate was the most active against R. solani. followed by diethylene glycol dibutanoate and ethylene glycol dibutanoate. Against S. rolfsii ethylene glycol diheptanoate was found to be most active followed by diethylene glycol diisobutanoate As compared to the standard reference benomyl (EC50 5.16 μg/mL), the potential alkanediol dialkanoates showed EC50 in the range of 33 – 60 μg/mL.

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Antifungal efficacy of chitosan nanoparticles against phytopathogenic fungi and inhibition of zearalenone production by Fusarium graminearum

Comunicata Scientiae ◽

10.14295/cs.v10i3.1899 ◽

2019 ◽

Vol 10 (3) ◽

pp. 338-345

Author(s):

Hanem A. Abdel-Aliem ◽

Ahmed Y. Gibriel ◽

Nagwa M. H. Rasmy ◽

Ahmed F. Sahab ◽

Aziza A. El-Nekeety ◽

...

Keyword(s):

Antifungal Activity ◽

Fusarium Graminearum ◽

Chitosan Nanoparticles ◽

Sclerotium Rolfsii ◽

Phytopathogenic Fungi ◽

Dependent Manner ◽

Amino Groups ◽

Deacetylation Degree ◽

Alternaria Tenuis ◽

Dose Dependent

Chitosan (COS) is a natural safe biopolymer that received great attention in agriculture, food, biomedical, pharmaceutical and environmental industries because their biocompatible, biodegradable, non-toxic and non-allergenic natures. The aims of the current study were to synthesize and characterize chitosan nanoparticles (COS-NPs), to evaluate their antifungal activity against phytopathogenic fungi and inhibition of zearalenone (ZEN) production by Fusarium graminearum. The results revealed that the deacetylation degree of COS was 86.9 0.44 %, the average of molar mass was 171.41 ± 0.29 g/mol, molecular weight was 244 ± 7 kDa and the concentration of free amino groups was 0.05 ± 0.019 mol L-1. COS-NPs showed the nanorod form with rough nature and particle size was around 180 nm. COS-NPs showed an excellent antifungal activity against Alternaria tenuis, Aspergillus niger, A. flavus, Baeuvaria bassiana, Fusarium graminearum, Fusarium oxysporum, Penicillium sp. and Sclerotium rolfsii in dose dependent manner. At a concentration of 800 ppm, it inhibits ZEN production by Fusarium graminearum. It could be concluded that COS-NPs are promise candidate as safe antifungal capable for the prevention of ZEN production.

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Synthesis, Antifungal Activity and Structure-Activity Relationships of Vanillin Oxime-N-O-Alkanoates

Natural Product Communications ◽

10.1177/1934578x1200701224 ◽

2012 ◽

Vol 7 (12) ◽

pp. 1934578X1200701 ◽

Cited By ~ 1

Author(s):

Vivek Ahluwalia ◽

Nandini Garg ◽

Birendra Kumar ◽

Suresh Walia ◽

Om P. Sati

Keyword(s):

Antifungal Activity ◽

Chain Length ◽

Acyl Chain ◽

Sclerotium Rolfsii ◽

Hydroxylamine Hydrochloride ◽

Macrophomina Phaseolina ◽

Phytopathogenic Fungi ◽

Acyl Chlorides ◽

Acyl Chain Length

Vanillin oxime- N-O-alkanoates were synthesized following reaction of vanillin with hydroxylamine hydrochloride, followed by reaction of the resultant oxime with acyl chlorides. The structures of the compounds were confirmed by IR, 1H, 13C NMR and mass spectral data. The test compounds were evaluated for their in vitro antifungal activity against three phytopathogenic fungi Macrophomina phaseolina, Rhizoctonia solani and Sclerotium rolfsii by the poisoned food technique. The moderate antifungal activity of vanillin was slightly increased following its conversion to vanillin oxime, but significantly increased after conversion of the oxime to oxime- N-O-alkanoates. While vanillin oxime- N-O-dodecanoate with an EC50 value 73.1 μg/mL was most active against M. phaseolina, vanillin oxime- N-O-nonanoate with EC50 of value 66.7 μg/mL was most active against R. solani. The activity increased with increases in the acyl chain length and was maximal with an acyl chain length of nine carbons.

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Antifungal activity of nano emulsions of neem and citronella oils against phytopathogenic fungi, Rhizoctonia solani and Sclerotium rolfsii

Industrial Crops and Products ◽

10.1016/j.indcrop.2017.06.061 ◽

2017 ◽

Vol 108 ◽

pp. 379-387 ◽

Cited By ~ 38

Author(s):

Eisa Osman Mohamed Ali ◽

Najam Akhtar Shakil ◽

Virendra Singh Rana ◽

Dhruba Jyoti Sarkar ◽

Sujan Majumder ◽

...

Keyword(s):

Antifungal Activity ◽

Rhizoctonia Solani ◽

Sclerotium Rolfsii ◽

Phytopathogenic Fungi

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Antifungal activity of dendritic cell lysosomal proteins against Cryptococcus neoformans

Scientific Reports ◽

10.1038/s41598-021-92991-6 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Benjamin N. Nelson ◽

Savannah G. Beakley ◽

Sierra Posey ◽

Brittney Conn ◽

Emma Maritz ◽

...

Keyword(s):

Antifungal Activity ◽

Cryptococcus Neoformans ◽

Mammalian Cells ◽

Cysteine Proteases ◽

Dependent Manner ◽

Life Threatening ◽

Opportunistic Fungal Pathogen ◽

Dose Dependent ◽

Lysosomal Proteins

AbstractCryptococcal meningitis is a life-threatening disease among immune compromised individuals that is caused by the opportunistic fungal pathogen Cryptococcus neoformans. Previous studies have shown that the fungus is phagocytosed by dendritic cells (DCs) and trafficked to the lysosome where it is killed by both oxidative and non-oxidative mechanisms. While certain molecules from the lysosome are known to kill or inhibit the growth of C. neoformans, the lysosome is an organelle containing many different proteins and enzymes that are designed to degrade phagocytosed material. We hypothesized that multiple lysosomal components, including cysteine proteases and antimicrobial peptides, could inhibit the growth of C. neoformans. Our study identified the contents of the DC lysosome and examined the anti-cryptococcal properties of different proteins found within the lysosome. Results showed several DC lysosomal proteins affected the growth of C. neoformans in vitro. The proteins that killed or inhibited the fungus did so in a dose-dependent manner. Furthermore, the concentration of protein needed for cryptococcal inhibition was found to be non-cytotoxic to mammalian cells. These data show that many DC lysosomal proteins have antifungal activity and have potential as immune-based therapeutics.

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In vitro Antifungal Activity of Plant Extracts, Hydrolates and Essential Oils of Some Medicinal Plants and Control of Cucumber Anthracnose

European Journal of Medicinal Plants ◽

10.9734/ejmp/2019/v28i430139 ◽

2019 ◽

pp. 1-9

Author(s):

Amanda P. Mattos ◽

Fabricio P. Povh ◽

Bruna B. Rissato ◽

Vítor V. Schwan ◽

Kátia R. F. Schwan-Estrada

Keyword(s):

Antifungal Activity ◽

Medicinal Plants ◽

Curcuma Longa ◽

Sclerotium Rolfsii ◽

Phytopathogenic Fungi ◽

In Vivo Test ◽

Activity Effect ◽

In Vitro Antifungal Activity

Aims: This study is aimed to evaluate the in vitro antifungal activity effect of the crude aqueous extract (CAE), hydrolate (HY) and essential oil (EO) of Corymbia citriodora, Cymbopogon citratus, Cymbopogon flexuosus and Curcuma longa against the phytopathogenic fungi Alternaria steviae, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Sclerotium rolfsii, and assess, in situ, the effectiveness of CAE of medicinal plants in reducing the severity of the cucumber anthracnose. Methodology: The EOs and HYs were obtained by hydrodistillation. The CAEs were prepared by the turbolysis method. Mycelial growth of the fungi was measured daily, by the diametrically opposite method. In the in vivo test, the CAEs were sprayed on the cotyledon leaves of healthy cucumber plants with three days after were inoculated with C. lagenarium. The severity of assessment of the disease was based on a scale of notes. Results: The medicinal plants studied showed antifungal activity against all or almost all pathogens. In general, treatment with CAE and HY of C. longa revealed the highest inhibition against the fungi tested. With the exception of the EO of C. longa, the other EOs showed total inhibition against all the fungi and in all the concentrations tested. Compared to control, in in vivo assays CAE of C. citratus presents a potential for control of cucumber anthracnose reducing the severity of the disease. Conclusion: The medicinal plants studied produce compounds associated with antimicrobial activity.

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Characterization and Antifungal Activity of Limonoid Constituents Isolated from Meliaceae Plants Melia dubia, Aphanamixis polystachya, and Swietenia macrophylla against Plant Pathogenic Fungi In Vitro

Journal of Chemistry ◽

10.1155/2021/4153790 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Thanh Nguyen Tan ◽

Hieu Tran Trung ◽

Quang Le Dang ◽

Hien Vu Thi ◽

Hoang Dinh Vu ◽

...

Keyword(s):

Antifungal Activity ◽

Biological Activities ◽

Sclerotium Rolfsii ◽

Phytopathogenic Fungi ◽

Phytophthora Species ◽

Plant Diseases ◽

Moderate Activity ◽

Swietenia Macrophylla ◽

Study Results ◽

Melia Dubia

The plants of Meliaceae are native to tropical and subtropical regions as the Americas, west India, Southeast Asia, and Southern China. Many species of the genera Khaya, Swietenia, Aphanamixis, and Melia in this family are known as medicinal plants and have biological activities such as antiviral, antimicrobial, antifeeding, insecticidal, and cytotoxic properties. The objectives of this research are to characterize and evaluate the bioactive limonoids from several plants of Meliaceae against phytopathogenic fungi. During the search of antifungal compounds from the plants of Meliaceae, the three methanol extracts of Melia dubia, Aphanamixis polystachya, and Swietenia macrophylla were found to suppress the mycelial growth of several phytopathogenic fungi. Nine limonoids isolated from M. dubia (1–2), A. polystachya (3–5), and S. macrophylla (6–9) were evaluated, for the first time, their antifungal effectiveness against nine phytopathogenic fungi Fusarium oxysporum, Magnaporthe oryzae, Sclerotium rolfsii, Rhizoctonia solani, Alternaria spp., and Botrytis cinerea, and three oomycetes Phytophthora species. Limonoids 2, 3, 6, and 8 displayed a remarkable broad-spectrum antifungal activity against all the test fungi. Sclerotium rolfsii was highly sensitive to the four limonoids with IC50 values ranging from 79.4 to 128.0 µg/mL. Notably, chisocheton compound G (3) isolated from A. polystachya and khayanolide B (8) isolated from S. macrophylla were the most potent antifungal limonoids and exhibited a dose-dependent activity against Phytophthora species. Compounds 2 and 9 displayed moderate activity against M. oryzae. Our study results demonstrated the discovery of antifungal and lead compounds from the group of limonoids for application in the control of fungal plant diseases.

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Strong Antifungal Activity of SS750, a New Triazole Derivative, Is Based on Its Selective Binding Affinity to Cytochrome P450 of Fungi

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.46.2.308-314.2002 ◽

2002 ◽

Vol 46 (2) ◽

pp. 308-314 ◽

Cited By ~ 10

Author(s):

Masaru Matsumoto ◽

Kazuya Ishida ◽

Akihiro Konagai ◽

Kazunori Maebashi ◽

Takemitsu Asaoka

Keyword(s):

Cytochrome P450 ◽

Antifungal Activity ◽

In Vivo Studies ◽

Dependent Manner ◽

Strong Affinity ◽

Dose Dependent ◽

Potent Inhibitory Activity ◽

In Vitro Antifungal Activity

ABSTRACT SS750 [(R)-(−)-2-(2,4-difluorophenyl)-1-(ethylsulfonyl)-1,1-difluoro-3-(1H-1,2,4-triazol-1-yl)-2-propanol] is a new triazole, and its potential as an antifungal agent was evaluated by in vitro and in vivo studies. In a comparison of the MICs at which 50% of isolates are inhibited (MIC50s) for all strains of Candida species and Cryptococcus neoformans tested, SS750 was four times or more active than fluconazole and had activity comparable to that of itraconazole. The most important advantage of SS750 was that, when the MIC90s were compared, SS750 had 64 and 32 times greater antifungal activities than fluconazole against Candida krusei and Candida glabrata, respectively, which are intrinsically less susceptible to fluconazole. In cyclophosphamide-immunosuppressed mouse models of systemic and pulmonary candidiasis caused by C. albicans, oral SS750 prolonged the number of days of survival of infected animals in a dose-dependent manner and was 4 and ≥64 times more potent than fluconazole and itraconazole, respectively. In a safety profile, SS750, like fluconazole, had less of an affinity for binding to mammalian cytochrome P450 compared with that of ketoconazole, despite its strong affinity for binding to fungal cytochrome P450. The mechanism for the increased in vitro antifungal activity of SS750 against C. krusei is partially due to the potent inhibitory activity (3.7 times versus that of fluconazole) of C. krusei cytochrome P450 sterol 14α-demethylase; SS750 showed a strong affinity for binding to cytochrome P450 of C. krusei, indicating that SS750 acts by inhibiting the cytochrome P450 sterol 14α-demethylase of fungal cells.

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Effective control of neem (Azadirachta indica A. Juss) cake to plant parasitic nematodes and fungi in black pepper diseases in vitro

Journal of Vietnamese Environment ◽

10.13141/jve.vol6.no3.pp233-238 ◽

2014 ◽

Vol 6 (3) ◽

pp. 233-238

Author(s):

Duc Hieu Duong ◽

Xuan Quang Ngo ◽

Dang Giap Do ◽

Thi Anh Hong Le ◽

Vu Thanh Nguyen ◽

...

Keyword(s):

Rhizoctonia Solani ◽

Phytophthora Capsici ◽

Larval Mortality ◽

Sclerotium Rolfsii ◽

Phytopathogenic Fungi ◽

Effective Control ◽

Neem Cake ◽

Second Stage ◽

Meloidogyne Spp ◽

Plant Parasitic

Neem cake is a product of the cold pressing from the neem kernels to obtain neem oil. Bio-active substances from neem cake extracted solutions were evaluated for their potential to control the root knot nematodes and other pests of plants. In this study different concentrations of the solution extracted from neem cake was tested against the second stage juveniles of the plant parasitic nematode Meloidogyne spp. and four phytopathogenic fungi: Rhizoctonia solani, Sclerotium rolfsii, Collectotrichum spp. and Phytopthora capsici. Toxicity of neem cake extractions is represented by the EC50 value for the second-stage juvenile (J2) of Meloidogyne spp. and the four phytopathogenic fungi via Probit analysis. A 5% dilution of the solvent extracting from neem cake already caused 100% larval mortality after 24 hours exposure. Undiluted neem cake extraction effectively inhibited the growth of the four phytopathogenic fungi. The EC50 value of neem cake on J2-larvae of Meloidogyne nematode and on the fungi Rhizoctonia solani, Sclerotium rolfsii, Collectotrichum spp. and Phytophthora capsici was 0.51, 0.74, 0.30, 0.51 and 4.33%, respectively. Bánh dầu neem là sản phẩm của quá trình ép nhân hạt neem để lấy dầu. Các hoạt chất sinh học từ dịch chiết bánh dầu neem đã được đánh giá có tiềm năng lớn trong phòng trừ tuyến trùng nốt sưng và các loài dịch hại khác của nhiều loại cây trồng. Trong nghiên cứu này các nồng độ dịch chiết khác nhau của bánh dầu neem đã được thử nghiệm khả năng diệt tuyến trùng (ấu trùng tuổi 2 thuộc giống Meloidogyne spp.) và ức chế 4 loài nấm bệnh như: Rhizoctonia solani, Sclerotium rolfsii, Collectotrichum spp. và Phytopthora capsici. Độc tính của dịch chiết bánh dầu neem được biểu diễn bởi giá trị EC50 đối với ấu trùng tuổi 2 của tuyến trùng Meloidogyne spp. và các loài nấm bệnh thông qua phân tích Probit. Dịch chiết bánh dầu neem ở nồng độ 5% đã làm chết 100% cá thể IJ2 của Meloidogyne spp sau 24 giờ phơi nhiễm. Dịch nguyên chất bánh dầu neem ức chế cả 4 loài nấm bệnh. Giá trị EC50 của bánh dầu neem lên ấu trùng tuổi 2 của Meloidogyne spp và các loài nấm bệnh Rhizoctonia solani, Sclerotium rolfsii, Collectotrichum spp. and Phytophthora capsici tương ứng là 0.51, 0.74, 0.30, 0.51 và 4.33%.

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In Vitro Antifungal Activity of Citrus aurantifolia Linn Plant Extracts against Phytopathogenic Fungi Macrophomina phaseolina

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.13.70 ◽

2014 ◽

Vol 13 ◽

pp. 70-74 ◽

Cited By ~ 1

Author(s):

S. Balamurugan

Keyword(s):

Antifungal Activity ◽

Root Rot ◽

Field Experiments ◽

Macrophomina Phaseolina ◽

Phytopathogenic Fungi ◽

Citrus Aurantifolia ◽

Dependent Inhibition ◽

Dose Dependent Inhibition ◽

Dose Dependent

The aqueous extracts of leaf of Citrus aurantifolia L were assessed in vitro for inhibitory activity against Macrophominaphaseolina isolated from dry root rot specimens of Gingelly. The antifungal activity was determined by poison food technique. The extracts have shown dose dependent inhibition of mycelial growth of test fungi. The extracts were more effective in inhibiting Macrophominaphaseolina. The extracts of Citrus aurantifolia were found effective against Gingelly dry root rot pathogens. Further field experiments are to be carried out to recommend the extracts against the disease.

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Copper Acyl Salicylate Has Potential as an Anti-CryptococcusAntifungal Agent

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02345-17 ◽

2018 ◽

Vol 62 (8) ◽

Cited By ~ 4

Author(s):

Adepemi O. Ogundeji ◽

Boitumelo F. Porotloane ◽

Carolina H. Pohl ◽

Pravin S. Kendrekar ◽

Olihile M. Sebolai

Keyword(s):

Antifungal Activity ◽

Metal Complex ◽

Growth Inhibition ◽

Metabolic Activity ◽

Immune Cells ◽

Dependent Manner ◽

Content Type ◽

Therapeutic Outcomes ◽

Dose Dependent

ABSTRACTThein vitroantifungal activity of aspirin against cryptococcal cells has been reported. However, the unwanted effects of aspirin may limit its clinical application. Conceivably, a derivative of aspirin could overcome this challenge. Toward this end, this study considered the usage of an aspirinate-metal complex, namely, copper acyl salicylate (CAS), as an anti-Cryptococcusantifungal agent. Additionally, the study examined the effects of this compound on macrophage function. Thein vitrosusceptibility results revealed that cryptococcal cells were vulnerable (in a dose-dependent manner) to CAS, which might have effected growth inhibition by damaging cryptococcal cell membranes. Interestingly, when CAS was used in combination with fluconazole or amphotericin B, synergism was observed. Furthermore, CAS did not negatively affect the growth or metabolic activity of macrophages; rather, it sensitized those immune cells to produce interferon gamma and interleukin 6, which, in turn, might have aided in the phagocytosis of cryptococcal cells. Compared to our aspirin data, CAS was noted to be more effective in killing cryptococcal cells (based on susceptibility results) and less toxic toward macrophages (based on growth inhibition results). Taking these findings together, it is reasonable to conclude that CAS may be a better anti-Cryptococcusdrug that could deliver better therapeutic outcomes, compared to aspirin.

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