Comparative analysis of flavonoids and polar metabolites from hairy roots of Scutellaria baicalensis and Scutellaria lateriflora

ABSTRACT: The hairy root culture of American Skullcap (Scutellaria lateriflora) was studied to investigate the biomass and flavonoids content (baicalin, baicalein and wogonin) in response of various auxin concentrations.The growth rates of the hairy roots varied significantly only at IBA 0.1 mg/L and for all other auxin treatments did not vary significantly. The biomass of hairy roots was 8% higher when treated with IBA 0.1 mg/L and biomass was almost similar and slightly lower levels when treated with various IAA concentration and NAA, respectively. However, the auxins treatments responsed positively to increase flavone production in American Skullcaphairy root culture. The auxin indole-3-butyric acid (IBA) at 1 mg/L performed the best for the accumulation of baicalin and wogonin. The auxin IBA at 1 mg/L accumulated 1.64 and 2.92 times higher baicalin and wogonin, respectively compared to control treatment. Meanwhile, the highest levels of baicalein were observed for hair root cultures in the presence of 1-naphthaleneacetic acid (NAA) at 0.1 mg/L achieving 2.38 times higher than that of accumulated in the control. These findings indicate that hairy root cultures of S. lateriflorausing liquid 1/2MS medium supplemented with auxin could be a valuable alternative approach for flavonoid production.

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Induction of specialized metabolites in hairy rootS of Scutellaria lateriflora treated with cyclodextrin and methyl jasmonate

Planta Medica ◽

10.1055/s-0032-1320340 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

Z Marsh ◽

L Nopo-Olazabal ◽

T Yang ◽

N Joshee ◽

F Medina-Bolivar

Keyword(s):

Methyl Jasmonate ◽

Hairy Roots ◽

Specialized Metabolites ◽

Scutellaria Lateriflora

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Overexpression of Cinnamate 4-Hydroxylase and 4-Coumaroyl CoA Ligase Prompted Flavone Accumulation in Scutellaria baicalensis Hairy Roots

Natural Product Communications ◽

10.1177/1934578x1400900618 ◽

2014 ◽

Vol 9 (6) ◽

pp. 1934578X1400900 ◽

Cited By ~ 1

Author(s):

Young Seon Kim ◽

Yeon Bok Kim ◽

YeJi Kim ◽

Mi Young Lee ◽

Sang Un Park

Keyword(s):

Metabolic Engineering ◽

Hairy Roots ◽

Single Gene ◽

Scutellaria Baicalensis ◽

Biosynthesis Pathway ◽

Upstream Gene ◽

Transcript Levels ◽

Scutellaria Baicalensis Georgi ◽

Coa Ligase ◽

Coenzyme A Ligase

Scutellaria baicalensis Georgi, a species of the Lamiaceae family, is considered as one of the 50 fundamental herbs used in traditional Chinese medicine. In order to enhance flavone (baicalein, baicalin, and wogonin) content in S. baicalensis roots, we overexpressed a single gene of cinnamate 4-hydroxylase (C4H) and 4-coumaroyl coenzyme A ligase (4CL) using an Agrobacterium rhizogenes-mediated system. SbC4H- and Sb4CL-overexpressed hairy root lines enhanced the transcript levels of SbC4H and Sb4CL compared with those in the control and also increased flavones contents by approximately 3- and 2.5-fold, respectively. We successfully engineered the flavone biosynthesis pathway for the production of beneficial flavones in S. baicalensis hairy roots. The importance of upstream gene C4H and 4CL in flavone biosynthesis and the efficiency of metabolic engineering in promoting flavone biosynthesis in S. baicalensis hairy roots have been indicated in this study.

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