Growth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Natasha Sant´Anna Iwanicki ◽  
Gabriel Moura Mascarin ◽  
Sara Giro Moreno ◽  
Jørgen Eilenberg ◽  
Italo Delalibera Júnior
Keyword(s):  
Nitrogen Source ◽  
Growth Kinetic ◽  
Liquid Culture ◽  
Dalbulus Maidis ◽  
Metarhizium Robertsii ◽  
Corn Leafhopper

Cell growth and pigment production in suspension cultures of a mycobiont isolated from the lichen Cladonia cristaiella

1995 ◽  
Vol 73 (S1) ◽  
pp. 590-594 ◽  
Author(s):  
Y. Yamamoto ◽  
Y. Kinoshita ◽  
T. Kurokawa ◽  
I. Yoshimura ◽  
V. Ahmadjian ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Liquid Culture ◽  
Pigment Production ◽  
Malt Extract ◽  
Nutritional Factors ◽  
Cultural Conditions ◽  
Acetone Extracts ◽  
Affect Cell Growth

This is the first study on the factors that affect cell growth and the production of secondary metabolites of a lichen mycobiont in liquid culture. An ascospore-derived strain of Cladonia cristatella mycobiont accumulated and excreted red pigments into a liquid medium. Growth of the mycobiont was increased by using liquid Lilly–Barnett medium containing 16% (w/v) sucrose as a carbon source, 0.2% (w/v) L-glutamine as a nitrogen source, and 0.2% (w/v) polypeptone, adjusting pH to 5.0 before autoclaving, and incubating cultures at 20 °C. Pigment production by the mycobiont was increased by using liquid Lilly–Barnett medium containing 4% (w/v) sucrose as a carbon source, 0.2% (w/v) L-asparagine as a nitrogen source, and 0.2% (w/v) malt extract, adjusting pH to 5.0 before autoclaving and incubating cultures at 20 °C. All acetone extracts under any cultural conditions yielded similar HPLC chromatograms. We proved no relationship between cell growth and secondary metabolism based on the nutritional factors in the cultured C. cristatella mycobiont. Key words: lichen, suspension culture, Cladonia cristatella mycobiont, red pigment, production, and growth factor.

Native parasitoids of Dalbulus elimatus and Dalbulus maidis (Hemiptera: Cicadellidae) in winter crops of central Mexico

2007 ◽  
Vol 139 (5) ◽  
pp. 722-727 ◽  
Author(s):  
Gustavo Moya-Raygoza
Keyword(s):  
Zea Mays ◽  
Hordeum Vulgare ◽  
Annual Cycle ◽  
Winter Season ◽  
Central Mexico ◽  
Dalbulus Maidis ◽  
First Report ◽  
Native Parasitoids ◽  
Winter Crops ◽  
Corn Leafhopper

AbstractNative parasitoids of the adult Mexican corn leafhopper, Dalbulus elimatus (Ball), and corn leafhopper, Dalbulus maidis (Delong and Wolcott), are described and their rates of parasitism on maize, Zea mays L., common barley, Hordeum vulgare L., and sorghum, Sorghumbicolor (L.) Moench (Poaceae) in central Mexico are determined. The survey was conducted throughout an annual cycle, with sampling of the previous season's crops when they were available. Leafhoppers showing evidence of parasitism were reared individually in the laboratory to obtain adult parasitoids. Adult D. elimatus were parasitized on winter maize, barley, and sorghum by the fly Eudorylas subopacus (Loew) (Diptera: Pipunculidae) and the wasp Gonatopus bartletti Olmi (Hymenoptera: Dryinidae), whereas adult D. maidis were parasitized by these fly and wasp parasitoids on winter maize only. The highest rates of parasitism of D. elimatus by E. subopacus were 57.4% on maize, 39.6% on barley, and 48.8% on sorghum. The highest rate of parasitism of D. maidis by E. subopacus was 20.4% on maize. In contrast, the highest rates of parasitism of D. elimatus by G. bartletti were 9.4% on maize, 2.7% on barley, and 25.8% on sorghum. The highest rate of parasitism of D. maidis by G. bartletti was 7.1% on maize. These results indicate that winter crops are habitats of E. subopacus and G. bartletti during the winter season. This is the first report of D. elimatus and D. maidis being parasitized by E. subopacus and of D. elimatus being parasitized by G. bartletti.

scholarly journals Accumulation of an unusual trichothecene shunt metabolite in liquid culture of Fusarium graminearum with methionine as the sole nitrogen source

2017 ◽  
Vol 67 (1) ◽  
pp. 7-9 ◽  
Author(s):  
Yuichi Nakajima ◽  
Yuya Tanaka ◽  
Kosuke Matsui ◽  
Kazuyuki Maeda ◽  
Yoshiyuki Kitou ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Fusarium Graminearum ◽  
Liquid Culture ◽  
Sole Nitrogen Source

scholarly journals Probing behavior of the corn leafhopper Dalbulus maidis on susceptible and resistant maize hybrids

2021 ◽  
Author(s):  
Pablo Carpane ◽  
María Inés Catalano
Keyword(s):  
Total Duration ◽  
Electrical Penetration Graph ◽  
Corn Hybrids ◽  
Dalbulus Maidis ◽  
Maize Hybrids ◽  
Probing Behavior ◽  
Corn Stunt ◽  
Corn Leafhopper ◽  
Old Females ◽  
Mesophyll Resistance

The corn leafhopper Dalbulus maidis is the main vector of the pathogens that cause corn stunt, a major disease of maize in the Americas. As host resistance is an efficient tool to control diseases, the findings of a previous report showed that some corn hybrids are resistant to D . maidis . In this work, we assessed the probing behavior of D . maidis on susceptible and resistant corn hybrids using EPG (Electrical Penetration Graph) technology. Fifteen-day-old females were monitored for 20 hours, with access to hybrids DK390, DK670, DK79-10, and DK72-10. Hybrids DK390 and DK72-10 showed resistance to D . maidis in phloem, since insects feeding on these hybrids presented more salivation events in phloem without subsequent ingestion, which are seen as failed attempts to ingest. A reduction of the total duration of phloem ingestion was observed, and accordingly of the time spent by insects with access to these hybrids on xylem ingestion. The hybrid DK390 also had mesophyll resistance, seen as less probing time and a higher number of probes of short duration. These findings support and are consistent with previous research, providing useful information to characterize maize hybrids resistant to D . maidis , and so to corn stunt.

Courtship Behavior of the Corn Leafhopper Dalbulus maidis (DeLong & Wolcott) (Hemiptera: Cicadellidae)

2014 ◽  
Vol 27 (6) ◽  
pp. 804-815 ◽  
Author(s):  
Ricardo Ramirez-Romero ◽  
Daniela Perez-Ascencio ◽  
Delia Garibay-Benítez
Keyword(s):  
Courtship Behavior ◽  
Dalbulus Maidis ◽  
Corn Leafhopper
Sign in / Sign up

Export Citation Format

Share Document