Cell growth and pigment production in suspension cultures of a mycobiont isolated from the lichen Cladonia cristaiella

1995 ◽  
Vol 73 (S1) ◽  
pp. 590-594 ◽  
Author(s):  
Y. Yamamoto ◽  
Y. Kinoshita ◽  
T. Kurokawa ◽  
I. Yoshimura ◽  
V. Ahmadjian ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Liquid Culture ◽  
Pigment Production ◽  
Malt Extract ◽  
Nutritional Factors ◽  
Cultural Conditions ◽  
Acetone Extracts ◽  
Affect Cell Growth

This is the first study on the factors that affect cell growth and the production of secondary metabolites of a lichen mycobiont in liquid culture. An ascospore-derived strain of Cladonia cristatella mycobiont accumulated and excreted red pigments into a liquid medium. Growth of the mycobiont was increased by using liquid Lilly–Barnett medium containing 16% (w/v) sucrose as a carbon source, 0.2% (w/v) L-glutamine as a nitrogen source, and 0.2% (w/v) polypeptone, adjusting pH to 5.0 before autoclaving, and incubating cultures at 20 °C. Pigment production by the mycobiont was increased by using liquid Lilly–Barnett medium containing 4% (w/v) sucrose as a carbon source, 0.2% (w/v) L-asparagine as a nitrogen source, and 0.2% (w/v) malt extract, adjusting pH to 5.0 before autoclaving and incubating cultures at 20 °C. All acetone extracts under any cultural conditions yielded similar HPLC chromatograms. We proved no relationship between cell growth and secondary metabolism based on the nutritional factors in the cultured C. cristatella mycobiont. Key words: lichen, suspension culture, Cladonia cristatella mycobiont, red pigment, production, and growth factor.

Research on microbial lipid production from potato starch wastewater as culture medium by Lipomyces starkeyi

2013 ◽  
Vol 67 (8) ◽  
pp. 1802-1808 ◽  
Author(s):  
Jun-Xian Liu ◽  
Qin-Yan Yue ◽  
Bao-Yu Gao ◽  
Yan Wang ◽  
Qian Li ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Culture Medium ◽  
Potato Starch ◽  
Inoculum Size ◽  
Microbial Lipid ◽  
Lipomyces Starkeyi ◽  
Starch Wastewater ◽  
Source Concentration

In this paper, potato starch wastewater as culture medium was treated by the oleaginous yeast Lipomyces starkeyi to biosynthesize microbial lipid. The result indicated that carbon source types, carbon source concentration, nitrogen source types, nitrogen source concentration, inoculum size, and cultivation time all had a significant effect on cell growth and microbial lipid accumulation in batch cultures. A measure of 120 g/L of glucose concentration, 3.0 g/L of (NH4)2SO4 concentration, 10% inoculum size, and incubation time 96 h cultivated in a shaking flask at 30 °C were found to be the optimal conditions not only for cell growth but also for lipid synthesis. Under this condition, the cellular biomass and lipid content could reach 2.59 g/L and 8.88%, respectively. This work provides a new method for effective utilization of potato starch wastewater, which has particular social and economic benefits for yeast treatment technology.

scholarly journals Characterization of protease producing fungi Aspergillus funiculosus and A. tamarii, and their proteases

2013 ◽  
pp. 91-98
Author(s):  
Z Shahina ◽  
MT Hossain ◽  
MA Hakim
Keyword(s):  
Enzyme Activity ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Substrate Concentration ◽  
Protease Activity ◽  
Maximal Activity ◽  
Environmental Condition ◽  
Nutritional Factors ◽  
Aspergillus Funiculosus

Two Aspergillus spp. namely A. funiculosus (Za) and A tamarii (Zc) were isolated, purified, identified and studied for their protease activity under some selected environmental condition and nutritional factors. The strains showed the maximal activity of protease after 5 days (Za) and 6 days (Zc) of incubation at 27ºC in a medium containing peptone as nitrogen source with pH 5.0. Isolate Za showed highest protease activity in the presence of fructose as carbon source, at 35 °C, with pH 6.0 and 2.5% casien as substrate concentration. Where as for Zc it was starch as carbon source, temperature 40 °C, with pH 7.0 and 2% casien ,the isolate showed highest enzyme activity. DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13390 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):91-98, 2009

scholarly journals Study of some effective factors on the production of garamicidin from locally isolated Bacillus brevis

2007 ◽  
Vol 4 (2) ◽  
pp. 215-220
Author(s):  
Baghdad Science Journal
Keyword(s):  
Carbon Source ◽  
Organic Solvent ◽  
Nitrogen Source ◽  
Ammonium Chloride ◽  
Bacillus Brevis ◽  
Effective Factors ◽  
Local Isolate ◽  
Cultural Conditions ◽  
Phosphate Source

The optimum cultural conditions for garamicidin production by local isolate B.brevis were studied.Best result was obtained when the isolate B.brevis was grown on media composed of 1%glucose as carbon source,1% ammonium chloride as a nitrogen source ,0.5% Dipotassium hydrogen orthophosphate as a phosphate source and after 48 hours of incubation at 30C .Garamicidin has been extracted and purified through acid precipition and then extracted by organic solvent (ether& acetone ).Using HPLC the garamicidin antibiotic showed three types A,B and C garamicidin .

scholarly journals ASPERGILLUS TERREUS KMBF1501 A POTENTIAL PIGMENT PRODUCER UNDER SUBMERGED FERMENTATION

2017 ◽  
Vol 9 (4) ◽  
pp. 38 ◽  
Author(s):  
P. Akilandeswari ◽  
B. V. Pradeep
Keyword(s):  
Nitrogen Source ◽  
Aspergillus Terreus ◽  
Inorganic Nitrogen ◽  
Pigment Production ◽  
High Yield ◽  
Dihydrogen Phosphate ◽  
Malt Extract ◽  
Mineral Salts ◽  
Potato Dextrose Broth ◽  
Inoculum Age

Objective: The present study was aimed to identify the fungal isolate from soil and to understand the different optimized parameters better to facilitate the pigment production that has high yield and stability.Methods: Aspergillus sp. was isolated from Western Ghats soil by the conventional serial dilution technique and assessed as a potential pigment producer. Different broth medium such as potato dextrose broth (PDB), czapek-dox broth (CDB), malt extract broth (MEB), rose bengal broth (RBB), sabouraud dextrose broth (SDB), yeast malt extract broth (YEMB), pH (3-9), temperature (24, 27, 30, 33, 37 and 40 °C), carbon (lactose,glucose,sucrose, maltose, galactose and fructose) and nitrogen source (peptone, yeast extract, urea and inorganic nitrogen sources like potassium nitrate, ammonium chloride and sodium nitrate), mineral salts such as sodium dihydrogen phosphate (Na2H2Po4), magnesium sulphate (Mg2So4), calcium chloride (CaCl2), copper sulphate (Cu2So4), potassium dihydrogen phosphate (KH2Po4) and manganese sulphate (Mn2So4) and inoculum age (2-7 d) of the medium related to high pigment production were analysed.Results: Aspergillus terreus KMBF1501 was identified by ribosomal DNA sequencing showing 99% similarity with other Aspergillus terreus and the Accession number (KX113516) was assigned. The optimum culture conditions for pigment production by Aspergillus terreus KMBF1501 was achieved at pH 5 (0.563±0.012 nm), temperature of 27 °C (0.382±0.001 nm) with glucose (0.501±0.002 nm) as carbon source, peptone (2.147±0.004 nm) as nitrogen source, Mg2SO4 (0.401±0.001 nm)as mineral salt and 4 d (0.324±0.001 nm) of inoculum age in PDB (0.761±0.006 nm).Conclusion: Aspergillus terreus KMBF1501 produced maximum pigment when cultured in modified PDB than in common PDB medium. The high concentration of the pigment can be used for various industrial purposes.

scholarly journals Study on culture conditionsfor extracellular alginate lyase production by Bacillus velezensis AlgSm1

2021 ◽  
Vol 63 (3) ◽  
pp. 19-23
Author(s):  
Thi Thuy Hang Cao ◽  
◽  
Mai Nhu Hieu Vo ◽  
Duc Thinh Pham ◽  
Ngoc Linh Nguyen ◽  
...  
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
High Activity ◽  
Marine Bacteria ◽  
Ph Value ◽  
Alginate Lyase ◽  
Bacillus Velezensis ◽  
Cultural Conditions ◽  
Nutritional Components ◽  
Initial Ph

Alginate lyase is an enzyme that degrades alginate to create bioactive oligoalginate for application in medicine, industry, agriculture... In this study, cultural conditions of marine bacteria, Bacillus velezensis AlgSm1 have been investigated for producing alginate lyase with high content and activity. The nutritional components such as carbon source, nitrogen source, initial pH value, and cultural time were investigated. The results showed that the cultural conditions of B. velezensis AlgSm1 to biosynthesize alginate lyase with high activity were 5 mg/ml alginate, 0.8 mg/ml yeast extract; pH 5.5 and 18-hours fermentation at 28-30°C.

Conversion of viridin to viridiol by viridin-producing fungi

1987 ◽  
Vol 33 (11) ◽  
pp. 963-966 ◽  
Author(s):  
Richard W. Jones ◽  
Joseph G. Hancock
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Production System ◽  
Liquid Culture ◽  
Calcium Nitrate ◽  
Peat Moss ◽  
Significant Extent ◽  
Gliocladium Virens ◽  
Culture Conversion ◽  
Simple Production

The fungistatic compound viridin, produced by the fungus Gliocladium virens, was found to be irreversibly reduced to the phytotoxin viridiol in liquid culture. Conversion occurred only in the presence of viridin-producing fungi and was subsequent to viridin production. Radiolabelled viridin was rapidly taken up by the mycelium of G. virens and reduced to radiolabelled viridiol, while labelled viridiol was not taken up to any significant extent by the mycelium. Reduction of viridin to viridiol was independent of culture pH, carbon source, and nitrogen source or quantity. A simple production system consisting of peat moss amended with dextrose and calcium nitrate and inoculated with G. virens supported production of 86 μg viridiol/g peat. This production system, when applied to soil, may have value as a herbicide.

scholarly journals Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla

2002 ◽  
Vol 45 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Aneli M. Barbosa ◽  
Cristina G. M. Souza ◽  
Robert F. H. Dekker ◽  
Rafael C. Fonseca ◽  
Dalva T. Ferreira
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Carbon Source ◽  
Host Plants ◽  
Culture Medium ◽  
Virulent Strain ◽  
Fungal Strain ◽  
Malt Extract ◽  
Mato Grosso

Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone or malt extract) to the culture medium did not influence phytotoxin production. The phytotoxin produced by Strain I was most active at pH 6.0, stable between pH 3-9, and was highly thermostable, remaining fully active when heated at 90 ° C for 1 h.

Studies on the Biological Characteristics of Termitomyces Albuminosus Hypha

2013 ◽  
Vol 709 ◽  
pp. 810-813 ◽  
Author(s):  
Xiong Ya ◽  
Min Jie Li
Keyword(s):  
Growth Factors ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Biological Characteristics ◽  
Edible Fungi ◽  
Growth Environment ◽  
Wild Edible Fungi ◽  
Development And Utilization

Termitomyces albuminosus is a kind of local distinctive wild edible fungi in southwest of China. It is delicious, rich in nutrition and has high development and utilization value, but owing to the restrictions of growth environment, it can not be cultivated artificially. This article mainly studied on the biological characteristics of Termitomyces albuminosus Hypha, and found out the optimal carbon source, nitrogen source, growth factors and the C/N ratio that are suitable for the growth of Hypha of Termitomyces albuminosus .

scholarly journals Monomethylamine as a Nitrogen Source for a Nonmethylotrophic Bacterium, Agrobacterium tumefaciens

2010 ◽  
Vol 76 (12) ◽  
pp. 4102-4104 ◽  
Author(s):  
Yin Chen ◽  
Kathryn L. McAleer ◽  
J. Colin Murrell
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Nitrogen Source ◽  
Key Enzymes ◽  
Genes Encoding

ABSTRACT Monomethylamine can be used by nonmethylotrophs as a sole nitrogen source but not as a carbon source; however, little is known about the genes and enzymes involved. The γ-glutamylmethylamide/N-methylglutamate pathway for monomethylamine utilization by methylotrophs has recently been resolved. We have identified genes encoding key enzymes of this pathway in nonmethylotrophs (e.g., Agrobacterium tumefaciens) and demonstrated that this pathway is also involved in the utilization of monomethylamine as a nitrogen source by nonmethylotrophs.

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