NMR based metabolomics study of Y2 receptor activation by neuropeptide Y in the SK-N-BE2 human neuroblastoma cell line

Metabolomics ◽  
2015 ◽  
Vol 11 (5) ◽  
pp. 1243-1252 ◽  
Author(s):  
Bo Wang ◽  
Sulaiman Sheriff ◽  
Ambikaipakan Balasubramaniam ◽  
Michael A. Kennedy
Keyword(s):  
Neuropeptide Y ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Receptor Activation ◽  
Neuroblastoma Cell Line ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Human Neuroblastoma Cell Line ◽  
Y2 Receptor ◽  
Metabolomics Study

scholarly journals Neuropeptide Y modulates ATP-induced increases in internal calcium via the adenylate cyclase/protein kinase A system in a human neuroblastoma cell line

1997 ◽  
Vol 321 (2) ◽  
pp. 439-444 ◽  
Author(s):  
Virginia SOARES LEMOS ◽  
Bernard BUCHER ◽  
Kenneth TAKEDA
Keyword(s):  
Protein Kinase ◽  
Neuropeptide Y ◽  
Protein Kinase A ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Human Neuroblastoma Cell Line ◽  
Kinase A

The modulatory effects of neuropeptide Y (NPY) on ATP-induced increases in cytosolic free-calcium concentration ([Ca2+]i) were investigated in the CHP-234 human neuroblastoma cell line. Pretreatment of cells with 100 nM NPY potentiated the increase in [Ca2+]i evoked subsequently by 20 ƁM ATP, compared with initial application of ATP in a control experiment, whereas a similar pretreatment with 1 ƁM NPY attenuated the subsequent response to ATP. Both actions of NPY were completely blocked by H-89 {N-[2-((3-(4-bromo-phenyl)-2-propenyl)-amino)-ethyl]-5 isoquinoline sulphonamide dihydrochloride}, a selective antagonist of protein kinase A. The effects of 100 nM NPY were mimicked by H-89, while forskolin and 8-Br-cAMP mimicked the effects of 1 ƁM NPY. Both basal and forskolin-stimulated cAMP levels were inhibited by 100 nM NPY and by 100 nM NPY(13-36), a selective agonist of the NPY Y2-receptor subtype. In contrast, at 1 ƁM such inhibition was not observed for either NPY or NPY(13-36). It is concluded that NPY has a biphasic modulatory effect on increases in [Ca2+]i produced by ATP, which probably involves the cAMP/protein kinase A cascade.

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